MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3526 799.7676 2396.2810
3527 600.2880 2397.1229
3528 801.3695 2401.0867
3529 801.4336 2401.2790
3530 602.0111 2404.0153
3531 482.0107 2405.0171
3532 483.2227 2411.0771
3533 805.0328 2412.0766
3534 604.0283 2412.0841
3535 604.0283 2412.0841
3536 483.4241 2412.0841
3537 483.4243 2412.0851
3538 604.0286 2412.0853
3539 604.0287 2412.0857
3540 604.0287 2412.0857
3541 484.6167 2418.0471
3542 484.6178 2418.0526
3543 605.5209 2418.0545
3544 484.6184 2418.0556
3545 484.6184 2418.0556
3546 605.5212 2418.0557
3547 605.5215 2418.0569
3548 486.0170 2425.0486
3549 486.2160 2426.0436
3551 812.8731 2435.5975
3552 611.7708 2443.0541
3554 612.0206 2444.0533
3555 489.8184 2444.0556
3556 816.2414 2445.7024
3557 817.4480 2449.3222
3558 491.8162 2454.0446
3559 491.8166 2454.0466
3560 491.8169 2454.0481
3561 491.8169 2454.0481
3562 495.2184 2471.0556
3563 495.2185 2471.0561
3564 618.7714 2471.0565
3565 825.0257 2472.0553
3566 619.0212 2472.0557
3567 495.4186 2472.0566
3568 619.0218 2472.0581
3569 495.4190 2472.0586
3570 619.0222 2472.0597
3571 825.0275 2472.0607
3572 619.2729 2473.0625
3573 827.1091 2478.3055
3574 838.8871 2513.6395
3575 844.0766 2529.2080
3577 633.8446 2531.3493
3578 635.0074 2536.0005
3579 635.5739 2538.2665
3580 635.5739 2538.2665
3581 847.0985 2538.2737
3582 848.4762 2542.4068
3583 510.0833 2545.3801
3584 637.3530 2545.3829
3585 510.2824 2546.3756
3586 637.6022 2546.3797
3587 856.4595 2566.3567
3588 860.7583 2579.2531
3589 860.7987 2579.3743
3590 867.6676 2599.9810
3591 522.8359 2609.1431
3592 522.8376 2609.1516
3593 523.0364 2610.1456
3594 436.0320 2610.1483
3596 436.0323 2610.1501
3597 436.0323 2610.1501
3600 523.2371 2611.1491
3601 436.1992 2611.1515
3602 872.4426 2614.3060
3603 872.4459 2614.3159
3604 872.4915 2614.4527
3605 438.8670 2627.1583
3606 526.4390 2627.1586
3607 877.0580 2628.1522
3608 658.0461 2628.1553
3609 526.6385 2628.1561
3610 658.0468 2628.1581
3611 526.6389 2628.1581
3612 526.6391 2628.1591
3613 526.6391 2628.1591
3614 439.0338 2628.1591
3615 526.6392 2628.1596
3616 658.0472 2628.1597
3617 439.0339 2628.1597
3618 526.6393 2628.1601
3619 526.6393 2628.1601
3620 526.6394 2628.1606
3621 658.0479 2628.1625
3622 658.2970 2629.1589
3623 439.2005 2629.1593
3624 658.2977 2629.1617
3625 877.9917 2630.9533
3626 881.9192 2642.7358
3628 666.5729 2662.2625
3629 666.8238 2663.2661
3630 533.6606 2663.2666
3631 533.6607 2663.2671
3632 533.6607 2663.2671
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