MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3403 753.2584 2256.7534
3404 566.2441 2260.9473
3405 755.7670 2264.2792
3406 454.4330 2267.1286
3407 378.8621 2267.1289
3408 454.4337 2267.1321
3409 567.7905 2267.1329
3410 568.0402 2268.1317
3411 379.0293 2268.1321
3412 379.0295 2268.1333
3413 454.6341 2268.1341
3416 568.0410 2268.1349
3418 568.0411 2268.1353
3419 379.0299 2268.1357
3420 568.0416 2268.1373
3421 454.6348 2268.1376
3422 568.2898 2269.1301
3423 454.8349 2269.1381
3424 759.0261 2274.0565
3425 759.7604 2276.2594
3426 760.7532 2279.2378
3427 761.2327 2280.6763
3429 573.0677 2288.2417
3430 763.9915 2288.9527
3431 575.5135 2298.0249
3432 767.4023 2299.1851
3433 461.4816 2302.3716
3434 461.6194 2303.0606
3435 461.6199 2303.0631
3436 576.8510 2303.3749
3437 461.8207 2304.0671
3438 769.7770 2306.3092
3439 770.3091 2307.9055
3440 770.3889 2308.1449
3441 771.6757 2312.0053
3442 579.0094 2312.0085
3443 579.9949 2315.9505
3444 581.0242 2320.0677
3445 465.0216 2320.0716
3447 581.2778 2321.0821
3448 465.2245 2321.0861
3452 581.5311 2322.0953
3455 777.0549 2328.1429
3456 1165.5758 2329.1370
3457 777.3865 2329.1377
3459 1170.0366 2338.0586
3461 585.8073 2339.2001
3462 585.8087 2339.2057
3463 781.0745 2340.2017
3464 782.3521 2344.0345
3465 587.0159 2344.0345
3467 782.4277 2344.2613
3468 785.6944 2354.0614
3470 589.7772 2355.0797
3471 589.7775 2355.0809
3473 786.9579 2357.8519
3475 787.6709 2359.9909
3476 787.6790 2360.0152
3477 591.0112 2360.0157
3478 473.0873 2360.4001
3479 591.1076 2360.4013
3480 473.0881 2360.4041
3481 473.0883 2360.4051
3482 591.1088 2360.4061
3483 473.4268 2362.0976
3484 473.4273 2362.1001
3486 473.4274 2362.1006
3487 473.4276 2362.1016
3488 473.4276 2362.1016
3489 473.6282 2363.1046
3490 592.2994 2365.1685
3491 791.0528 2370.1366
3492 593.7753 2371.0721
3493 1186.5647 2371.1148
3494 792.0768 2373.2086
3496 476.6264 2378.0956
3498 794.0377 2379.0913
3499 595.7834 2379.1045
3500 476.8290 2379.1086
3501 397.5255 2379.1093
3502 595.7847 2379.1097
3505 596.0347 2380.1097
3506 477.0293 2380.1101
3507 477.0293 2380.1101
3509 596.0350 2380.1109
3510 794.3778 2380.1116
3511 477.0296 2380.1116
3512 596.0352 2380.1117
3513 596.0352 2380.1117
3514 477.0297 2380.1121
3515 596.0354 2380.1125
3516 477.0311 2380.1191
3517 596.2834 2381.1045
3518 794.7107 2381.1103
3519 477.2301 2381.1141
3521 479.4951 2392.4391
3522 479.8215 2394.0711
3523 479.8216 2394.0716
3524 479.8216 2394.0716
3525 479.8221 2394.0741
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