MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3166 633.4824 1897.4254
3167 633.4859 1897.4359
3168 633.6227 1897.8463
3169 633.9510 1898.8312
3170 475.7724 1899.0605
3171 950.9472 1899.8798
3173 956.9611 1911.9076
3174 479.2055 1912.7929
3175 479.4560 1913.7949
3176 958.5844 1915.1542
3177 959.3361 1916.6576
3178 641.6477 1921.9213
3179 642.0046 1922.9920
3180 482.4980 1925.9629
3182 643.2937 1926.8593
3183 644.1495 1929.4267
3185 644.9417 1931.8033
3186 483.9590 1931.8069
3187 484.4934 1933.9445
3188 387.7963 1933.9451
3189 387.7964 1933.9456
3190 387.7964 1933.9456
3192 484.4942 1933.9477
3193 484.7455 1934.9529
3194 484.9608 1935.8141
3195 388.1972 1935.9496
3196 485.2480 1936.9629
3197 485.4849 1937.9105
3198 485.7144 1938.8285
3199 647.6512 1939.9318
3200 971.0286 1940.0426
3201 647.6891 1940.0455
3202 647.9762 1940.9068
3204 973.2145 1944.4144
3206 651.1525 1950.4357
3209 489.4622 1953.8197
3210 652.2806 1953.8200
3211 977.9661 1953.9176
3212 652.9518 1955.8336
3213 489.9664 1955.8365
3214 653.2740 1956.8002
3215 653.6041 1957.7905
3216 979.9582 1957.9018
3218 655.6000 1963.7782
3219 656.6874 1967.0404
3220 656.9642 1967.8708
3221 493.1969 1968.7585
3223 657.9335 1970.7787
3224 658.2844 1971.8314
3226 494.2197 1972.8497
3227 330.0608 1974.3211
3228 495.2587 1977.0057
3229 496.2926 1981.1413
3231 397.5979 1982.9531
3232 496.7458 1982.9541
3233 662.0339 1983.0799
3234 992.5480 1983.0814
3235 992.5490 1983.0834
3236 993.4657 1984.9168
3237 662.8284 1985.4634
3238 663.6100 1987.8082
3240 664.7064 1991.0974
3241 499.4539 1993.7865
3242 499.7384 1994.9245
3243 665.9845 1994.9317
3244 499.9904 1995.9325
3245 501.2632 2001.0237
3246 668.3200 2001.9382
3247 668.3485 2002.0237
3248 1006.9952 2011.9758
3249 672.8240 2015.4502
3250 505.9525 2019.7809
3251 674.6032 2020.7878
3253 1015.7187 2029.4228
3254 680.1721 2037.4945
3255 1021.4918 2040.9690
3256 409.8177 2044.0521
3257 512.9526 2047.7813
3259 683.6962 2048.0668
3260 412.8537 2059.2321
3261 687.7731 2060.2975
3262 413.2190 2061.0586
3263 413.2191 2061.0591
3264 413.2191 2061.0591
3265 413.2191 2061.0591
3266 344.5172 2061.0595
3267 413.2192 2061.0596
3268 413.2197 2061.0621
3269 344.6838 2062.0591
3270 413.4192 2062.0596
3271 413.4196 2062.0616
3272 413.4196 2062.0616
3273 413.4197 2062.0621
3274 344.6844 2062.0627
3275 413.4204 2062.0656
3276 689.9788 2066.9146
3278 691.0247 2070.0523
3279 416.4565 2077.2461
3280 416.4565 2077.2461
3281 520.3189 2077.2465
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