MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2808 783.8770 1565.7394
2809 523.5972 1567.7698
2810 523.6068 1567.7986
2811 785.3886 1568.7626
2812 523.9282 1568.7628
2813 525.5808 1573.7206
2814 525.9145 1574.7217
2816 788.3693 1574.7240
2817 788.9183 1575.8220
2818 395.2163 1576.8361
2820 790.4073 1578.8000
2822 791.1914 1580.3682
2823 528.6047 1582.7923
2825 529.2417 1584.7033
2826 793.3868 1584.7590
2827 793.4462 1584.8778
2829 794.8768 1587.7390
2830 530.2995 1587.8767
2831 398.4687 1589.8457
2834 531.5836 1591.7290
2836 531.5841 1591.7305
2837 796.8726 1591.7306
2838 531.5843 1591.7311
2839 531.5845 1591.7317
2840 531.5847 1591.7323
2841 796.8736 1591.7326
2842 796.8967 1591.7788
2843 797.3715 1592.7284
2844 531.9182 1592.7328
2845 532.2507 1593.7303
2846 399.4417 1593.7377
2847 399.4418 1593.7381
2848 532.2745 1593.8017
2849 532.2798 1593.8176
2850 532.3029 1593.8869
2851 400.4263 1597.6761
2853 536.2366 1605.6880
2854 804.2354 1606.4562
2855 402.9593 1607.8081
2856 537.5941 1609.7605
2857 805.8917 1609.7688
2858 537.5989 1609.7749
2859 537.6039 1609.7899
2860 806.3931 1610.7716
2861 537.9388 1610.7946
2862 323.1835 1610.8811
2863 404.6833 1614.7041
2864 808.3635 1614.7124
2865 539.5701 1615.6885
2866 539.9047 1616.6923
2867 810.2482 1618.4818
2868 540.6039 1618.7899
2869 540.9312 1619.7718
2870 540.9315 1619.7727
2871 405.9507 1619.7737
2873 324.9621 1619.7741
2874 324.9622 1619.7746
2875 405.9510 1619.7749
2876 405.9514 1619.7765
2877 812.8549 1623.6952
2878 542.2405 1623.6997
2879 542.2412 1623.7018
2880 542.9321 1625.7745
2881 542.9377 1625.7913
2882 815.8441 1629.6736
2883 326.9424 1629.6756
2884 408.4265 1629.6769
2886 544.2330 1629.6772
2888 544.2333 1629.6781
2889 544.2616 1629.7630
2890 816.1436 1630.2726
2891 544.6066 1630.7980
2894 545.9484 1634.8234
2895 546.2371 1635.6895
2896 818.8525 1635.6904
2897 409.9305 1635.6929
2898 409.9498 1635.7701
2899 546.3436 1636.0090
2900 410.0097 1636.0097
2901 546.3445 1636.0117
2902 819.2355 1636.4564
2903 410.2415 1636.9369
2904 820.3401 1638.6656
2905 548.2557 1641.7453
2906 548.2595 1641.7567
2907 548.6335 1642.8787
2908 412.1911 1644.7353
2909 549.5565 1645.6477
2910 412.4193 1645.6481
2912 550.2268 1647.6586
2913 550.5905 1648.7497
2915 550.5974 1648.7704
2916 825.3945 1648.7744
2917 413.2079 1648.8025
2919 825.8972 1649.7798
2920 413.4579 1649.8025
2921 826.5466 1651.0786
2922 413.9436 1651.7453
2923 413.9460 1651.7549
2924 551.9341 1652.7805
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