MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2690 485.2431 1452.7075
2692 727.8759 1453.7372
2693 727.9367 1453.8588
2694 364.7026 1454.7813
2696 485.9357 1454.7853
2697 728.5705 1455.1264
2698 487.5363 1459.5871
2699 730.8822 1459.7498
2700 732.3516 1462.6886
2701 732.3519 1462.6892
2702 488.5705 1462.6897
2703 732.3805 1462.7464
2704 488.9046 1463.6920
2705 489.2167 1464.6283
2706 367.4176 1465.6413
2707 734.8058 1467.5970
2708 490.2396 1467.6970
2710 734.8777 1467.7408
2711 491.8716 1472.5930
2712 491.9375 1472.7907
2713 492.1978 1473.5716
2715 492.2690 1473.7852
2716 738.3270 1474.6394
2717 493.2579 1476.7519
2718 493.2655 1476.7747
2719 370.2010 1476.7749
2720 493.5930 1477.7572
2722 495.5656 1483.6750
2724 496.6044 1486.7914
2725 496.8900 1487.6482
2726 373.2372 1488.9197
2727 373.2373 1488.9201
2728 373.2374 1488.9205
2729 373.2376 1488.9213
2730 373.2379 1488.9225
2731 497.9166 1490.7280
2732 373.6903 1490.7321
2733 746.5660 1491.1174
2734 498.5525 1492.6357
2735 747.3261 1492.6376
2736 498.5582 1492.6528
2737 750.8187 1499.6228
2738 501.9351 1502.7835
2739 501.9356 1502.7850
2740 376.7068 1502.7981
2741 376.9571 1503.7993
2742 377.1973 1504.7601
2743 502.5940 1504.7602
2745 503.9142 1508.7208
2746 503.9167 1508.7283
2748 756.3955 1510.7764
2749 506.2239 1515.6499
2750 506.5390 1516.5952
2751 507.5951 1519.7635
2752 507.8715 1520.5927
2753 507.9119 1520.7139
2755 509.2677 1524.7813
2756 382.9529 1527.7825
2757 382.9640 1527.8269
2758 382.9648 1527.8301
2759 382.9652 1527.8317
2760 383.4561 1529.7953
2761 510.9395 1529.7967
2762 383.4565 1529.7969
2763 510.9399 1529.7979
2764 510.9541 1529.8405
2765 511.2008 1530.5806
2766 383.6529 1530.5825
2767 767.8989 1533.7832
2771 385.6912 1538.7357
2772 513.9219 1538.7439
2773 770.5970 1539.1794
2774 771.0317 1540.0488
2775 514.5730 1540.6972
2776 386.6854 1542.7125
2777 773.3804 1544.7462
2778 773.3873 1544.7600
2779 515.9285 1544.7637
2780 775.8854 1549.7562
2782 518.2750 1551.8032
2783 518.5920 1552.7542
2785 518.5962 1552.7668
2787 519.2524 1554.7354
2788 519.2944 1554.8614
2789 389.9533 1555.7841
2790 520.2554 1557.7444
2791 520.2600 1557.7582
2792 780.1666 1558.3186
2793 780.8850 1559.7554
2794 520.9269 1559.7589
2795 520.9269 1559.7589
2796 390.9569 1559.7985
2797 390.9576 1559.8013
2798 390.9576 1559.8013
2799 391.4495 1561.7689
2800 521.5972 1561.7698
2801 521.5993 1561.7761
2802 391.4531 1561.7833
2803 521.6208 1561.8406
2806 522.2936 1563.8590
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