MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2569 324.6601 1294.6113
2571 325.1849 1296.7105
2572 325.1851 1296.7113
2573 433.2445 1296.7117
2574 433.2920 1296.8542
2575 650.8197 1299.6248
2577 434.2587 1299.7543
2578 650.9866 1299.9586
2579 651.3193 1300.6240
2580 434.5502 1300.6288
2581 652.3985 1302.7824
2582 655.3240 1308.6334
2583 438.9314 1313.7724
2584 658.2954 1314.5762
2585 329.6882 1314.7237
2586 439.5828 1315.7266
2588 443.1758 1326.5056
2589 664.2870 1326.5594
2590 333.1784 1328.6845
2593 671.3365 1340.6584
2594 448.8958 1343.6656
2596 449.2459 1344.7159
2597 449.8988 1346.6746
2598 449.9048 1346.6926
2599 337.6808 1346.6941
2600 674.3546 1346.6946
2601 449.9061 1346.6965
2602 451.2680 1350.7822
2603 679.8654 1357.7162
2604 680.1693 1358.3240
2605 681.3044 1360.5942
2606 454.5712 1360.6918
2608 455.2485 1362.7237
2609 683.0470 1364.0794
2610 343.4349 1369.7105
2614 343.9390 1371.7269
2615 459.2505 1374.7297
2616 459.9035 1376.6887
2617 345.4666 1377.8373
2618 345.4668 1377.8381
2619 345.4671 1377.8393
2620 345.4672 1377.8397
2621 345.4672 1377.8397
2623 691.4001 1380.7856
2624 347.1679 1384.6425
2625 462.5548 1384.6426
2626 347.9419 1387.7385
2627 463.5875 1387.7407
2628 694.9069 1387.7992
2629 697.3494 1392.6842
2630 697.8230 1393.6314
2631 465.5642 1393.6708
2633 698.3566 1394.6986
2634 465.9073 1394.7001
2635 465.9073 1394.7001
2636 468.5834 1402.7284
2639 351.9321 1403.6993
2640 468.9075 1403.7007
2641 351.9325 1403.7009
2643 703.3386 1404.6626
2644 469.2467 1404.7183
2645 470.5730 1408.6972
2646 471.0854 1410.2344
2647 706.3537 1410.6928
2648 471.2385 1410.6937
2649 706.3548 1410.6950
2650 709.9774 1417.9402
2652 473.9154 1418.7244
2653 473.9186 1418.7340
2654 473.9501 1418.8285
2657 474.2560 1419.7462
2658 476.2237 1425.6493
2659 476.5521 1426.6345
2660 714.3430 1426.6714
2661 714.3507 1426.6868
2662 476.8782 1427.6128
2663 476.9608 1427.8606
2664 716.9102 1431.8058
2665 478.5547 1432.6423
2666 359.1687 1432.6457
2667 478.9100 1433.7082
2668 479.0494 1434.1264
2669 479.2552 1434.7438
2670 718.8204 1435.6262
2671 718.8211 1435.6276
2672 479.5641 1435.6705
2673 479.5645 1435.6717
2674 359.9252 1435.6717
2675 359.9256 1435.6733
2676 479.8843 1436.6311 1436.6585 -19.1 1     +0Score > 21 indicates identity
Score > 13 indicates homology		 DHDGYYWIRGR
2677 719.9320 1437.8494
2680 481.5416 1441.6030
2681 361.4086 1441.6053
2682 722.3434 1442.6722
2683 723.8439 1445.6732
2685 484.9085 1451.7037
2686 726.8653 1451.7160
2687 484.9138 1451.7196
2688 485.0744 1452.2014
2689 485.0756 1452.2050
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