MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2446 541.4242 1080.8338
2447 541.8469 1081.6792
2448 541.9999 1081.9852
2449 361.8430 1082.5072
2450 542.2610 1082.5074
2452 362.2302 1083.6688
2454 362.8100 1085.4082
2456 544.2917 1086.5688
2457 544.2930 1086.5714
2458 363.8491 1088.5255
2459 1090.0920 1089.0847
2460 546.3287 1090.6428
2461 1099.4601 1098.4528
2462 367.1670 1098.4792
2464 368.1943 1101.5611
2465 551.7880 1101.5614
2466 553.2850 1104.5554
2467 555.2628 1108.5110
2468 370.5118 1108.5136
2469 370.8839 1109.6299
2470 1114.9641 1113.9568
2471 1117.2465 1116.2392
2472 373.4673 1117.3801
2473 559.6983 1117.3820
2474 374.2164 1119.6274
2475 374.8469 1121.5189
2476 375.1745 1122.5017
2477 562.2585 1122.5024
2478 1123.5299 1122.5226
2479 375.5276 1123.5610
2481 563.7979 1125.5812
2483 378.5661 1132.6765
2484 380.4985 1138.4737
2485 570.7465 1139.4784
2486 571.0677 1140.1208
2487 571.0679 1140.1212
2488 381.1710 1140.4912
2489 381.1895 1140.5467
2490 381.2622 1140.7648
2491 573.2471 1144.4796
2493 385.1848 1152.5326
2494 577.2794 1152.5442
2497 387.2451 1158.7135
2498 583.0485 1164.0824
2499 583.7869 1165.5592
2500 588.2805 1174.5464
2501 392.8277 1175.4613
2502 589.8077 1177.6008
2503 590.0267 1178.0388
2504 393.9093 1178.7061
2505 393.9097 1178.7073
2506 591.0336 1180.0526
2508 594.8171 1187.6196
2509 596.0372 1190.0598
2510 598.3033 1194.5920
2515 302.8696 1207.4493
2516 604.8223 1207.6300
2518 607.3319 1212.6492
2519 405.2242 1212.6508
2521 607.4673 1212.9200
2522 405.5768 1213.7086
2523 608.3627 1214.7108
2524 406.2564 1215.7474
2525 609.1271 1216.2396
2526 612.2692 1222.5238
2527 409.2726 1224.7960
2528 409.2736 1224.7990
2529 615.8066 1229.5986
2530 410.8891 1229.6455
2531 411.5366 1231.5880
2532 411.8881 1232.6425
2533 412.1871 1233.5395
2534 619.8976 1237.7806
2535 413.6009 1237.7809
2536 619.8979 1237.7812
2537 620.7989 1239.5832
2538 622.2950 1242.5754
2539 622.3501 1242.6856
2540 312.4012 1245.5757
2542 624.2951 1246.5756
2544 625.1374 1248.2602
2545 627.3522 1252.6898
2547 421.9125 1262.7157
2548 316.9393 1263.7281
2549 637.1027 1272.1908
2550 425.1868 1272.5386
2551 637.3506 1272.6866
2552 637.4100 1272.8054
2553 425.8936 1274.6590
2555 639.8311 1277.6476
2557 427.9175 1280.7307
2558 321.1998 1280.7701
2559 321.2002 1280.7717
2560 427.9312 1280.7718
2561 427.9313 1280.7721
2562 321.2003 1280.7721
2563 644.8336 1287.6526
2564 645.2852 1288.5558
2565 646.3243 1290.6340
2568 432.5437 1294.6093
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