MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4447)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2328 304.5320 910.5742
2329 304.5323 910.5751
2330 912.0883 911.0810
2331 912.5181 911.5108
2332 456.7810 911.5474
2333 913.1822 912.1749
2334 913.1828 912.1755
2335 305.1582 912.4528
2336 914.2810 913.2737
2338 306.8563 917.5471
2339 920.4360 919.4287
2340 921.8491 920.8418
2341 308.0907 921.2503
2343 308.8128 923.4166
2344 463.2036 924.3926
2345 312.8223 935.4451
2346 469.0744 936.1342
2347 469.0747 936.1348
2348 469.0750 936.1354
2349 469.0750 936.1354
2350 938.4424 937.4351
2351 313.4968 937.4686
2353 471.0859 940.1572
2354 314.6815 941.0227
2355 943.4442 942.4369
2356 945.0707 944.0634
2358 946.1492 945.1419
2359 473.6694 945.3242
2360 476.0683 950.1220
2361 476.0687 950.1228
2362 317.8203 950.4391
2363 951.4496 950.4423
2365 477.7997 953.5848
2366 955.2301 954.2228
2367 478.2708 954.5270
2368 956.4513 955.4440
2369 478.7629 955.5112
2370 956.5186 955.5113
2372 957.9578 956.9505
2374 959.0572 958.0499
2375 959.0575 958.0502
2377 961.4753 960.4680
2378 324.1481 969.4225
2379 971.5317 970.5244
2380 975.0285 974.0212
2381 325.8643 974.5711
2382 976.9655 975.9582
2383 978.3438 977.3365
2384 978.4659 977.4586
2385 326.8346 977.4820
2386 489.7538 977.4930
2387 326.8759 977.6059
2388 490.7421 979.4696
2389 982.4147 981.4074
2390 328.4933 982.4581
2391 492.7741 983.5336
2392 492.8149 983.6152
2393 984.6227 983.6154
2394 492.8151 983.6156
2395 328.8792 983.6158
2396 328.8795 983.6167
2397 328.8800 983.6182
2398 985.9193 984.9120
2399 495.1255 988.2364
2400 495.1260 988.2374
2401 992.4609 991.4536
2402 331.8525 992.5357
2403 497.7359 993.4572
2404 332.1604 993.4594
2405 998.4754 997.4681
2406 333.8829 998.6269
2407 500.7452 999.4758
2409 335.0387 1002.0943
2411 505.7225 1009.4304
2412 337.4845 1009.4317
2413 340.8795 1019.6167
2414 340.8796 1019.6170
2415 341.8589 1022.5549
2416 342.5045 1024.4917
2417 342.8600 1025.5582
2418 343.1906 1026.5500
2419 343.9060 1028.6962
2420 516.2732 1030.5318
2422 518.7648 1035.5150
2423 519.3051 1036.5956
2424 520.2195 1038.4244
2425 1039.4341 1038.4268
2426 520.2629 1038.5112
2429 1045.5668 1044.5595
2430 523.2871 1044.5596
2433 525.7758 1049.5370
2434 352.1523 1053.4351
2436 352.1525 1053.4357
2437 352.5174 1054.5304
2438 528.5781 1055.1416
2439 353.8827 1058.6263
2440 356.5169 1066.5289
2441 534.2780 1066.5414
2444 357.1797 1068.5173
2445 358.2304 1071.6694
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