MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2090 348.7120 695.4094
2091 349.7147 697.4148
2092 699.0186 698.0113
2093 699.0192 698.0119
2094 699.0198 698.0125
2095 701.4974 700.4901
2096 704.3311 703.3238
2097 705.6993 704.6920
2098 707.3451 706.3378
2099 355.1736 708.3326
2101 713.0076 712.0003
2102 713.0076 712.0003
2103 713.0082 712.0009
2104 713.0083 712.0010
2105 713.0085 712.0012
2106 357.6233 713.2320
2108 714.9510 713.9437
2109 714.9512 713.9439
2110 714.9512 713.9439
2111 718.7607 717.7534
2112 362.0097 722.0048
2113 362.0098 722.0050
2114 362.0977 722.1808
2115 362.0977 722.1808
2116 362.0981 722.1816
2117 362.1926 722.3706
2118 724.4123 723.4050
2119 724.4128 723.4055
2120 724.4129 723.4056
2121 724.4130 723.4057
2122 362.7102 723.4058
2123 362.7103 723.4060
2124 362.7103 723.4060
2125 362.7405 723.4664
2127 725.4310 724.4237
2128 363.2195 724.4244
2131 731.1148 730.1075
2132 731.1149 730.1076
2133 366.7213 731.4280
2134 733.3471 732.3398
2135 736.7032 735.6959
2136 369.4514 736.8882
2137 369.6759 737.3372
2141 745.1044 744.0971
2142 373.1194 744.2242
2143 747.1416 746.1343
2144 376.6736 751.3326
2145 377.6809 753.3472
2146 757.4833 756.4760
2147 379.7126 757.4106
2148 758.4181 757.4108
2151 760.4169 759.4096
2152 761.3887 760.3814
2154 763.6083 762.6010
2155 764.3733 763.3660
2156 765.8325 764.8252
2157 766.3724 765.3651
2158 767.1080 766.1007
2160 385.6348 769.2550
2161 770.2625 769.2552
2162 770.2625 769.2552
2163 771.3738 770.3665
2164 771.3774 770.3701
2165 772.3744 771.3671
2166 387.2308 772.4470
2167 773.7072 772.6999
2168 387.7417 773.4688
2169 388.0478 774.0810
2170 388.0479 774.0812
2171 389.9532 777.8918
2172 390.6732 779.3318
2173 781.0951 780.0878
2174 781.0967 780.0894
2175 785.3734 784.3661
2176 393.7285 785.4424
2177 393.7468 785.4790
2178 394.7124 787.4102
2179 789.3771 788.3698
2181 793.3781 792.3708
2182 795.3805 794.3732
2183 796.3688 795.3615
2184 398.7327 795.4508
2185 797.0892 796.0819
2188 399.1944 796.3742
2189 797.3819 796.3746
2190 799.1753 798.1680
2192 401.2107 800.4068
2193 802.0649 801.0576
2194 802.2347 801.2274
2195 802.3884 801.3811
2196 802.4324 801.4251
2198 806.3801 805.3728
2199 404.2230 806.4314
2200 808.4123 807.4050
2201 810.9022 809.8949
2202 811.3768 810.3695
2203 406.2199 810.4252
2204 407.7442 813.4738
2205 816.2016 815.1943
2206 410.1706 818.3266
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