MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1776 485.0739 484.0666
1777 485.0745 484.0672
1778 485.0746 484.0673
1779 485.0748 484.0675
1780 485.0750 484.0677
1781 485.0751 484.0678
1782 486.3051 485.2978
1783 487.8033 486.7960
1784 489.9446 488.9373
1785 489.9449 488.9376
1786 489.9449 488.9376
1787 489.9454 488.9381
1788 490.9685 489.9612
1789 490.9685 489.9612
1790 490.9692 489.9619
1791 491.0908 490.0835
1792 491.7108 490.7035
1793 493.0197 492.0124
1794 493.0198 492.0125
1795 493.0200 492.0127
1796 493.0200 492.0127
1797 493.0201 492.0128
1798 493.0203 492.0130
1799 493.0203 492.0130
1800 493.0205 492.0132
1801 493.0205 492.0132
1802 493.0207 492.0134
1803 493.0207 492.0134
1804 493.0207 492.0134
1805 493.2382 492.2309
1806 495.9616 494.9543
1807 495.9624 494.9551
1808 495.9625 494.9552
1809 501.0486 500.0413
1810 501.0487 500.0414
1811 501.0491 500.0418
1812 501.0504 500.0431
1813 504.9088 503.9015
1814 504.9092 503.9019
1815 504.9093 503.9020
1816 504.9094 503.9021
1817 507.2459 506.2386
1818 508.2783 507.2710
1819 508.9845 507.9772
1820 510.8277 509.8204
1821 511.1117 510.1044
1822 511.1120 510.1047
1823 515.0389 514.0316
1824 515.0395 514.0322
1825 515.0397 514.0324
1826 515.3315 514.3242
1829 518.5887 517.5814
1830 522.2614 521.2541
1831 524.2484 523.2411
1832 525.1276 524.1203
1833 525.2902 524.2829
1834 526.1928 525.1855
1835 526.1930 525.1857
1836 529.3111 528.3038
1837 531.0125 530.0052
1838 531.9180 530.9107
1839 533.0328 532.0255
1840 533.1194 532.1121
1841 533.1194 532.1121
1842 533.1197 532.1124
1843 533.1201 532.1128
1844 533.1205 532.1132
1845 542.9407 541.9334
1846 542.9409 541.9336
1847 544.9302 543.9229
1848 545.0637 544.0564
1849 554.3063 553.2990
1850 555.1005 554.0932
1851 555.1006 554.0933
1852 555.1009 554.0936
1853 555.1012 554.0939
1854 555.1032 554.0959
1855 555.2494 554.2421
1856 555.2538 554.2465
1857 557.0772 556.0699
1858 557.0772 556.0699
1859 557.0778 556.0705
1860 557.0780 556.0707
1861 557.0780 556.0707
1862 557.0781 556.0708
1863 557.0782 556.0709
1864 557.0782 556.0709
1865 557.0783 556.0710
1866 557.5106 556.5033
1867 558.6628 557.6555
1868 561.3255 560.3182
1869 569.1167 568.1094
1870 569.1172 568.1099
1871 569.1176 568.1103
1872 569.3167 568.3094
1873 570.2610 569.2537
1874 571.0660 570.0587
1875 571.0662 570.0589
1876 571.0664 570.0591
1877 571.0668 570.0595
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