MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
776 347.1849 346.1776
777 347.1850 346.1777
778 347.1850 346.1777
779 347.1850 346.1777
780 347.1850 346.1777
781 347.1850 346.1777
782 347.1852 346.1779
783 349.0723 348.0650
784 349.0727 348.0654
785 349.0728 348.0655
786 349.0729 348.0656
787 349.1417 348.1344
788 349.1420 348.1347
789 349.1420 348.1347
790 349.1799 348.1726
791 349.1801 348.1728
792 349.1805 348.1732
793 349.1806 348.1733
794 349.1810 348.1737
795 349.1815 348.1742
796 349.1841 348.1768
797 349.1844 348.1771
798 349.1997 348.1924
799 349.1999 348.1926
800 349.1999 348.1926
801 349.2000 348.1927
802 349.2000 348.1927
803 349.2000 348.1927
804 349.2002 348.1929
805 349.2002 348.1929
806 349.2003 348.1930
807 349.2003 348.1930
808 349.2003 348.1930
809 349.2003 348.1930
810 349.2004 348.1931
811 349.2004 348.1931
812 349.2004 348.1931
813 349.2005 348.1932
814 349.2006 348.1933
815 349.2006 348.1933
816 349.2007 348.1934
817 349.2007 348.1934
818 349.2007 348.1934
819 349.2010 348.1937
820 349.2203 348.2130
821 349.2206 348.2133
822 349.2208 348.2135
823 349.2210 348.2137
824 349.2211 348.2138
825 349.2213 348.2140
826 349.2214 348.2141
827 349.2215 348.2142
828 349.2216 348.2143
829 349.2217 348.2144
830 350.0587 349.0514
831 350.0587 349.0514
832 351.0425 350.0352
833 351.0426 350.0353
834 351.0429 350.0356
835 351.0432 350.0359
836 351.1614 350.1541
837 351.1621 350.1548
838 351.1974 350.1901
839 351.1974 350.1901
840 351.1974 350.1901
841 351.1979 350.1906
842 351.1980 350.1907
843 351.1982 350.1909
844 351.1982 350.1909
845 351.1984 350.1911
846 351.2160 350.2087
847 351.2160 350.2087
848 351.2160 350.2087
849 351.2161 350.2088
850 351.2161 350.2088
851 351.2163 350.2090
852 351.2163 350.2090
853 351.2164 350.2091
854 351.2164 350.2091
855 351.2165 350.2092
856 351.2166 350.2093
857 351.2361 350.2288
858 351.2364 350.2291
859 351.2364 350.2291
860 351.2365 350.2292
861 351.2365 350.2292
862 351.2365 350.2292
863 351.2367 350.2294
864 351.2367 350.2294
865 351.2367 350.2294
866 351.2367 350.2294
867 351.2368 350.2295
868 352.2194 351.2121
869 353.1770 352.1697
870 353.1771 352.1698
871 353.1772 352.1699
872 353.1773 352.1700
873 353.1773 352.1700
874 353.1774 352.1701
875 353.2196 352.2123
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