MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4447)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
576 333.2051 332.1978
577 333.2051 332.1978
578 333.2052 332.1979
579 333.2052 332.1979
580 333.2052 332.1979
581 333.2052 332.1979
582 333.2052 332.1979
583 333.2052 332.1979
584 333.2052 332.1979
585 333.2055 332.1982
586 333.2055 332.1982
587 333.2055 332.1982
588 333.2055 332.1982
589 333.2056 332.1983
590 333.2057 332.1984
591 333.2057 332.1984
592 333.2057 332.1984
593 333.2057 332.1984
594 333.2260 332.2187
595 333.2263 332.2190
596 333.2264 332.2191
597 333.2265 332.2192
598 335.0381 334.0308
599 335.0384 334.0311
600 335.0387 334.0314
601 335.0388 334.0315
602 335.0391 334.0318
603 335.2205 334.2132
604 335.2206 334.2133
605 335.2206 334.2133
606 335.2206 334.2133
607 335.2207 334.2134
608 335.2207 334.2134
609 335.2208 334.2135
610 335.2209 334.2136
611 335.2209 334.2136
612 335.2209 334.2136
613 335.2209 334.2136
614 335.2210 334.2137
615 335.2210 334.2137
616 335.2212 334.2139
617 335.2213 334.2140
618 335.2213 334.2140
619 335.2213 334.2140
620 335.2214 334.2141
621 335.2214 334.2141
622 335.2214 334.2141
623 335.2215 334.2142
624 335.2399 334.2326
625 335.2425 334.2352
626 335.9286 334.9213
627 335.9899 334.9826
628 336.2248 335.2175
629 337.0266 336.0193
630 337.0269 336.0196
631 337.0270 336.0197
632 337.0271 336.0198
633 337.0272 336.0199
634 337.0274 336.0201
635 337.0274 336.0201
636 337.0275 336.0202
637 337.0277 336.0204
638 337.1452 336.1379
639 337.1454 336.1381
640 337.1818 336.1745
641 337.1823 336.1750
642 337.1823 336.1750
643 337.1824 336.1751
644 337.1824 336.1751
645 337.1824 336.1751
646 337.1825 336.1752
647 337.1825 336.1752
648 337.1825 336.1752
649 337.1826 336.1753
650 337.1826 336.1753
651 337.1826 336.1753
652 337.1827 336.1754
653 337.1827 336.1754
654 337.1827 336.1754
655 337.2003 336.1930
656 337.2568 336.2495
657 337.2570 336.2497
658 337.9854 336.9781
659 337.9857 336.9784
660 337.9858 336.9785
661 337.9858 336.9785
662 337.9860 336.9787
663 337.9860 336.9787
664 337.9860 336.9787
665 337.9861 336.9788
666 337.9861 336.9788
667 337.9861 336.9788
668 337.9862 336.9789
669 337.9863 336.9790
670 337.9866 336.9793
671 338.1643 337.1570
672 338.1649 337.1576
673 338.1650 337.1577
674 338.1651 337.1578
675 338.1652 337.1579
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