MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
476 323.1844 322.1771
477 323.1844 322.1771
478 323.1844 322.1771
479 323.1844 322.1771
480 323.1846 322.1773
481 323.1846 322.1773
482 323.1847 322.1774
483 323.1847 322.1774
484 323.1848 322.1775
485 323.1848 322.1775
486 323.1849 322.1776
487 323.1849 322.1776
488 323.1850 322.1777
489 323.1850 322.1777
490 323.2032 322.1959
491 323.2046 322.1973
492 323.2776 322.2703
493 324.0844 323.0771
494 325.0435 324.0362
495 325.1136 324.1063
496 325.1139 324.1066
497 325.1140 324.1067
498 325.1448 324.1375
499 325.1450 324.1377
500 325.1455 324.1382
501 325.1633 324.1560
502 325.1635 324.1562
503 325.1636 324.1563
504 325.1636 324.1563
505 325.1818 324.1745
506 325.1820 324.1747
507 325.1821 324.1748
508 325.1822 324.1749
509 325.1822 324.1749
510 325.1823 324.1750
511 325.1824 324.1751
512 325.1825 324.1752
513 325.1825 324.1752
514 325.1826 324.1753
515 325.1828 324.1755
516 325.2005 324.1932
517 325.2008 324.1935
518 325.2014 324.1941
519 325.2206 324.2133
520 325.2210 324.2137
521 325.2865 324.2792
522 326.1479 325.1406
523 326.1483 325.1410
524 327.1614 326.1541
525 327.1617 326.1544
526 327.1620 326.1547
527 327.1621 326.1548
528 327.1793 326.1720
529 327.1798 326.1725
530 327.1979 326.1906
531 327.1986 326.1913
532 327.1999 326.1926
533 327.2023 326.1950
534 327.2165 326.2092
535 327.2165 326.2092
536 328.2342 327.2269
537 328.9934 327.9861
538 329.0385 328.0312
539 329.0386 328.0313
540 330.0597 329.0524
541 330.0598 329.0525
542 330.0600 329.0527
543 330.0602 329.0529
544 330.0602 329.0529
545 330.0602 329.0529
546 330.0604 329.0531
547 330.0604 329.0531
548 330.0605 329.0532
549 330.0605 329.0532
550 330.0606 329.0533
551 330.0608 329.0535
552 330.8640 329.8567
553 330.8643 329.8570
554 330.8644 329.8571
555 330.8645 329.8572
556 331.0340 330.0267
557 331.0342 330.0269
558 331.0925 330.0852
559 331.1895 330.1822
560 331.1898 330.1825
561 331.1898 330.1825
562 331.1898 330.1825
563 331.1899 330.1826
564 331.1900 330.1827
565 331.1900 330.1827
566 331.2103 330.2030
567 331.2107 330.2034
568 333.0097 332.0024
569 333.0099 332.0026
570 333.0101 332.0028
571 333.1533 332.1460
572 333.1694 332.1621
573 333.2045 332.1972
574 333.2050 332.1977
575 333.2051 332.1978
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