Match to: SYNJ1_BOVIN Score: 31 Expect: 1.9e+002 Synaptojanin-1 (EC 3.1.3.36) (Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1) (p150) (Fragme Nominal mass (Mr): 146492; Calculated pI value: 7.37 NCBI BLAST search of SYNJ1_BOVIN against nr Unformatted sequence string for pasting into other applications Taxonomy: Bos taurus Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 18 Number of mass values matched: 5 Sequence Coverage: 5% Matched peptides shown in Bold Red 1 MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK 51 GTYSKVLDAY GLLGVLRLNL GDIMLHYLVL VTGCMSVGKI QESEVFRVTS 101 TEFISLRVDS SDEDRISEVR KVLNSGNFYF AWSASGVSLD LSLNAHRSLQ 151 EHTTDNRFSW NQSLHLHLKH YGVNCADWLL RLMCGGVEIR TIYAAHKQAK 201 ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVVYLD DSVSSFIQIR 251 GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK NLYGKQIIVN 301 LLGSKEGEHM LSKAFQSHLK ASEHAADIQM VNFDYHQMVK GGKAEKLHSV 351 LKPQVQKFLD YGIFHFDGSE VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE 401 MLTKQLEALG LAEKPQLVTR FQEVFRSMWS VNGDSISKIY AGTGALEGKA 451 KLKDGARSVS RTIQNNFFDS SKQEAIDVLL LGNTLNSDLA DKARALLTTG 501 SLRVSEQTLQ SASSKVLKSM CENFYKYSKP KKIRVCVGTW NVNGGKQFRS 551 IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PMDIFPIGFE EMVELNAGNI 601 VNASTTNQKL WAAELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI 651 RDVAVDTVKT GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER 701 NDDFLEIARK LSFPMGRLLF SHDYVFWCGD FNYRIDLPNE EVKELIRQQN 751 WDSLIAGDQL INQKNAGQIF RGFLEGKVTF APTYKYDLFS DDYDTSEKCR 801 TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT WTPGTLLHYG 851 RAELKTSDHR PVVALIDIDI FEVEAEERQN IYKEVIAVQG PPDGTVLVSI 901 KSSLPENNFF NDALIDELLQ QFTNFGEVIL IRFVEDKMWV TFLEGSSALN 951 VLNLNGKELL GRTITITLKS PDWIKTLEEE MSLEKINVPL PSSTSSTLLG 1001 EDAEVTADFD MEGDVDDYSA EVEEILPQHL QPSSSSALAR PPVLHPGPVP 1051 ASHLPYRRGP VPSLPVRPSR APSRTPGPPA SQSSPVDTLP ATQLQQKDSS 1101 QTLEPKRPPP PRPVAPPARP APPQRPPPPS GARSPAPARE RVWSTRKAQE 1151 RPRRDNLGGS QLPPQGGLPG PGLAGHSAAR PIIPPRAGVI SAPESHGRVS 1201 AGRLTPESQR KTXEVLKGPA LLPEPLKPQA ALPVPPSLAP PSQEMQEPLI 1251 AVAAPLAQSA LQPSLETPPQ PPPRSRSSHS LPSDAPAAAA GATIRVTGEK 1301 QTGVSAVRLD CPLKSDPFED LSLN
Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 26 - 46 2265.1100 2264.1027 2264.0817 0.0211 1 R.HKEECLMFESGAVAVLSSAEK.E 462 - 472 1300.4700 1299.4627 1299.6095 -0.1468 0 R.TIQNNFFDSSK.Q 519 - 531 1624.7400 1623.7327 1623.7425 -0.0098 1 K.SMCENFYKYSKPK.K 856 - 878 2653.3900 2652.3827 2652.3395 0.0432 0 K.TSDHRPVVALIDIDIFEVEAEER.Q 1204 - 1211 958.3500 957.3427 957.5243 -0.1816 1 R.LTPESQRK.T No match to: 814.4300, 1000.3300, 1165.3900, 1182.4400, 1191.5000, 1320.4000, 1348.4100, 1355.5300, 1423.5200, 1426.5700, 2544.4100, 2550.3000, 2882.5000
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 26 - 46 2265.1100 2264.1027 2264.0817 0.0211 1 R.HKEECLMFESGAVAVLSSAEK.E 462 - 472 1300.4700 1299.4627 1299.6095 -0.1468 0 R.TIQNNFFDSSK.Q 519 - 531 1624.7400 1623.7327 1623.7425 -0.0098 1 K.SMCENFYKYSKPK.K 856 - 878 2653.3900 2652.3827 2652.3395 0.0432 0 K.TSDHRPVVALIDIDIFEVEAEER.Q 1204 - 1211 958.3500 957.3427 957.5243 -0.1816 1 R.LTPESQRK.T No match to: 814.4300, 1000.3300, 1165.3900, 1182.4400, 1191.5000, 1320.4000, 1348.4100, 1355.5300, 1423.5200, 1426.5700, 2544.4100, 2550.3000, 2882.5000
Mascot Search Results Mascot Search Results