Mascot Search Results

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Search title    : 
MS data file    : Interleukin_ECD_2.mgf
Database        : SwissProt 51.6 (257964 sequences; 93947433 residues)
Timestamp       : 15 Jul 2008 at 15:47:40 GMT



Protein hits    : IL11_HUMAN Interleukin-11 precursor (IL-11) (Adipogenesis inhibitory factor) (AGIF) (Oprelvekin) - Homo sapiens (Human)

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 39 indicate peptides with significant homology.
Individual ions scores > 63 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    IL11_HUMAN    Mass: 21416    Score: 257    Matches: 1(1)  Sequences: 1(1)
 Interleukin-11 precursor (IL-11) (Adipogenesis inhibitory factor) (AGIF) (Oprelvekin) - Homo sapiens (Human)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
1   19036.0750   19035.0677   19035.5678   -0.5000 0  263  5.3e-022 1  U    P.GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL.-

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : None
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2.2 Da
Fragment Mass Tolerance: ± 0.9 Da
Max Missed Cleavages   : 1
Instrument type        : FTMS-ECD
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
+MS2(19036.075)
Score greater than 39 indicates homology
Score greater than 63 indicates identity
Score    Expect     Delta  Hit  Protein     Peptide
263.1  5.3e-022   -0.5000    1   IL11_HUMAN P.GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL.-
 10.5  9.7e+003    1.7936                   N.VFIKCDYNHAINEIWYYHHVKGSLLQHNFLPIDSVAHTNCPSTGIKYPPKGKVAHPTLTTKTTTGTSTTIHNPTGIPARSYVHLSGKSGCLISNGKWYTSGTCATYHFKKTIDDSNIEIRSSKGICGINGNEEFTCSRAVSGGTDFQIKDGKIGYQDKFDWCFGSTTGSGSTAQT.S
  9.1  1.4e+004    1.0960                   R.FIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEE.D
  8.0  1.7e+004    0.4110                   L.AISLARLGKKVGLIDADIYGFSVPDMMGITVRPTIEGEKLLPVERFGVKVMSMGFFVEENAPVVWRGPMLGKMLNNFFHEVEWGEVDYIVLDLPPGTGDVALDVHTMLPSCKEIIVSTPHPTAAFVAARAGSMAIKTDHEVVGVIENMAYYESAKTGEREYVFGKGGGDKLAEEL.N
  7.4    2e+004    1.1705                   Q.AVVKDVKKAPNGQHLHTAEVTIGEISTGQQASARIEVRERLDIVKNHTATHLLHQALKDVLGEHVNQAGSLVSSERLRFDFSHFGQVTPNELQQIEEIVNEKVWQALPVDISIQRLEEAKAAGAMALFGEKYGSEVRVVRVGDYSLELCGGCHVRNTAEIGLFKITSESGIGAGV.R
  7.3    2e+004    1.7157                   L.KHLQDIKVKFSDPGQPMSFVLEFHFEPNDYFTNPVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFSPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEE.G
  7.3    2e+004    0.7258                   R.QKKRISEYSDHCTKWYNSGLSRLNGSTYEQWINYNRFRREMILMALDLVAVFPFHDPRRYSMETSTQLTREVYTDPVSLSISNPDIGPSFSQMENTAIRTPHLVDYLDELYIYTSKYKAFSHEIQPDLFYWSAHKVSFKKSEQSNLYTTGIYGKTSGYISSGA.Y
  6.8  2.3e+004    0.7167                   H.PECPEGFYKSDFGCQKCHHYCKTCNDAGPLACTSCPPHSMLDGGLCMECLSSQYYDTTSATCKTCHDSCRSCFGPGQFSCKGCVPPLHLDQLNSQCVSCCQNQTLAEKTSSAACCNCDGETGECKATSTGGKRRTVVGSGSAYKSSESKHGSFENDGNAREFVLRLDSPLTAITAIAVAI.C
  6.7  2.3e+004    0.8077                   P.SSTLCPICKTSDLTSTSSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALSPAHSPAKQPLGKPEQERSRSPGATQSGPRQAEAARATSVPGPTQATAPPEVGRVSPQPPLSTKPSTAEPRPPAGEAQGKSATTVPSGLGAAEQTQGGLTG.K
  6.4  2.5e+004    1.3212                   V.PFPALPAALPAANGPVPGPYGLPPQAGSSDSLSTSKSPPGKSSLGLDNSLSTSSEDPHSGCPSRSSSSLSFHSTPPPLPLLQQSPATLPLALPGAPAPLPPQPQNGLGRAPGAAGLGAMPMAEGLLGGLAGSGGLPLNGLLGGLNGAAAPNPASLSQAGGAPTLQLPGCLNSLTEQQRHLLQQQEQQLQQLQQL.L