Mascot Search Results

User            : 
Email           : 
Search title    : Example for Mascot Top Down search from Bruker ECD instrument
MS data file    : MYOGLOBIN_ECD.mgf
Database        : SwissProt 51.6 (257964 sequences; 93947433 residues)
Timestamp       : 15 Jul 2008 at 14:53:18 GMT



Protein hits    : MYG_EQUBU Myoglobin - Equus burchelli (Plains zebra) (Equus quagga)

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 25 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    MYG_EQUBU    Mass: 17072    Score: 406    Matches: 1(1)  Sequences: 1(1)
 Myoglobin - Equus burchelli (Plains zebra) (Equus quagga)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   %   Miss Score Expect Rank Unique  Peptide
1   16947.8540   16946.8467   16940.9649   0.0347 0  406  5.3e-040 1  U    M.GLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG.-

 
      Proteins matching the same set of peptides:
      MYG_HORSE    Mass: 17072    Score: 406    Matches: 1(1)  Sequences: 1(1)
 Myoglobin - Equus caballus (Horse)

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : NoCleave
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.1 %
Fragment Mass Tolerance: ± 0.1 Da
Max Missed Cleavages   : 1
Instrument type        : FTMS-ECD
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
1: Scan 1 (rt=0) [Y:\bruker_top_down\MYOGLOBIN_ECD.d]
Score greater than 25 indicates identity
Score    Expect       %    Hit  Protein    Peptide
405.7  5.3e-040    0.0347    1   MYG_EQUBU M.GLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG.-
 19.9       0.2    0.0824                  M.GLSDGEWQLVLNAWGKVETDIGGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGTTVLTALGNILKKKGQHEAELAPLAQSHATKHKIPVKYLEFISEAIIQVLESKHPGDFGADAQGAMSKALELFRNDIAAKYKELGFQG.-
 15.3      0.58   -0.0597                  M.GLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKTEDEMKASEDLKKHGNTVLSALGGILKKKGQHEAEIKPLAQSHATKHKIPVKYLEFISEAIIQVLQSKHPGDFGADAQAAMSKALELFRNDIAAKYKELGFQG.-
  3.9       8.1    0.0877                  M.GLSDGEWQLVLNVWGKVEADVGGHGQEVLIRLFTGHPETLEKFDKFKHLKTADEMKASEDLKKHGTTVLTALGGILKKKGQHEAELKPLAQSHATKHKIPIKYLEFISDAIVHVLHSKHPAEFGADAQAAMKKALELFRNDIAAKYKELGFQG.-
  1.1        15    0.0580                  M.GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGQHEAQLKPLAQSHATKHKIPVKYLEFISEVIIQVLQSKHPGDFGADAQGAMGKALELFRNDIAAKYKELGFQG.-