Mascot Search Results

User            : 
Email           : 
Search title    : Negative ion example
MS data file    : W:\mascot_test_data\bruker_negative_ions\Bombesin_LIFTnegativ\0_G11\0\1617.8067.LIFT_1.LIFT\fast\pdata\1\peaklist.xml.txt
Database        : NCBInr 20040418 (2768002 sequences; 778013805 residues)
Taxonomy        : lobe-finned fish and tetrapod clade (308940 sequences)
Timestamp       : 16 Mar 2005 at 17:54:37 GMT



Protein hits    : gi|229340 bombesin


  gi|29752274 hepatic nuclear factor 4 alpha [Homo sapiens]


  gi|110254 Ig heavy chain V region - mouse


  gi|4762004 immunoglobulin lambda light chain variable region [Homo sapiens]


  gi|18033113 

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 27 indicate peptides with significant homology.
Individual ions scores > 40 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    gi|229340    Mass: 1638     Score: 44     Matches: 1(1)  Sequences: 1(1)
 bombesin
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
1   1617.8066   1618.8139   1618.8150   -0.0011 1  44  0.025 1  U    -.EQRLGNQWAVGHLM.- + Amide (C-term); Pyro-glu (N-term E)

2.    gi|29752274    Mass: 1620     Score: 12     Matches: 1(0)  Sequences: 1(0)
 hepatic nuclear factor 4 alpha [Homo sapiens]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1617.8066   1618.8139   1618.8024   0.0115 0  12  41 2  U    -.MVSVNAPLGAPVESSY.- + Amide (C-term)

3.    gi|110254    Mass: 13538    Score: 12     Matches: 1(0)  Sequences: 1(0)
 Ig heavy chain V region - mouse
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1617.8066   1618.8139   1618.8790   -0.0652 0  12  42 3  U    K.LVESGGGLVQPHASLR.L

4.    gi|4762004    Mass: 4349     Score: 10     Matches: 1(0)  Sequences: 1(0)
 immunoglobulin lambda light chain variable region [Homo sapiens]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1617.8066   1618.8139   1618.6510   0.1629 0  10  62 4  U    R.SNAXGAVPDLXIYYD.-

5.    gi|18033113    Score: 7      Matches: 1(0)  Sequences: 1(0)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1617.8066   1618.8139   1618.8327   -0.0189 1  7  1.1e+002 5  U    M.EGALAERVGAEPFHR.V + Amide (C-term); Pyro-glu (N-term E)

 
      Proteins matching the same set of peptides:
      gi|20589949    Score: 7      Matches: 1(0)  Sequences: 1(0)

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Variable modifications : Amide (C-term),Pyro-glu (N-term E)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.3 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : MALDI-TOF-TOF
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 27 indicates homology
Score greater than 40 indicates identity
Score    Expect     Delta  Hit  Protein      Peptide
 43.8     0.025   -0.0011    1   gi|229340   -.EQRLGNQWAVGHLM.-
 11.5        41    0.0115    2   gi|29752274 -.MVSVNAPLGAPVESSY.-
 11.5        42   -0.0652    3   gi|110254   K.LVESGGGLVQPXASLR.L
  9.8        62    0.1629    4   gi|4762004  R.SNAXGAVPDLXIYYD.-
  7.3  1.1e+002   -0.0189    5   gi|18033113 M.EGALAERVGAEPFHR.V
  5.6  1.6e+002   -0.0764                    -.EVQLVESGGGVVRPGR.S
  5.6  1.6e+002   -0.1743                    R.LLDQLPRANVVLLR.Y
  5.5  1.7e+002   -0.1015                    R.LQVEGVQSVPPRSPK.Q
  4.4  2.1e+002   -0.0652                    M.EAALAVTRLPPNDPR.T
  4.2  2.2e+002   -0.0944                    -.XKLLVIPIDGSHWL.-