Mascot Search Results

User            : 
Email           : 
Search title    : Example of MassLynx processing
MS data file    : C:\MASSLYNX\Proteinlynx.pro\PKL_files\matilda_test_mass.txt
Database        : NCBInr 20050424 (2451907 sequences; 830525235 residues)
Taxonomy        : Firmicutes (gram-positive bacteria) (197715 sequences)
Timestamp       : 27 Apr 2005 at 18:57:45 GMT
Top Score       : 227 for Mixture 1, gi|16077150 + gi|16077703

Mascot Score Histogram

Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 65 are significant (p<0.05).

Score Distribution

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits

  

1.    Mixture 1    Total score: 227    Expect: 3.9e-018  Matches: 34
 Components (only one family member shown for each component):
      gi|16077150    Mass: 57503    Score: 149    Expect: 2.5e-010  Matches: 21
 lysyl-tRNA synthetase [Bacillus subtilis subsp. subtilis str. 168]
      gi|16077703    Mass: 57915    Score: 97     Expect: 3.6e-005  Matches: 16
 GMP synthetase [Bacillus subtilis subsp. subtilis str. 168]

2.    gi|16077150    Mass: 57503    Score: 149    Expect: 2.5e-010  Matches: 21
 lysyl-tRNA synthetase [Bacillus subtilis subsp. subtilis str. 168]
3.    Mixture 2    Total score: 145    Expect: 6.3e-010  Matches: 27
 Components (only one family member shown for each component):
      gi|143012    Mass: 57971    Score: 75     Expect: 0.006  Matches: 13
 GMP synthetase
      gi|7707810    Mass: 73020    Score: 68     Expect: 0.035  Matches: 14
 penicillin binding protein 3 [Bacillus cereus]
4.    gi|16077703    Mass: 57915    Score: 97     Expect: 3.6e-005  Matches: 16
 GMP synthetase [Bacillus subtilis subsp. subtilis str. 168]
      gi|143012    Mass: 57971    Score: 75     Expect: 0.006  Matches: 13
 GMP synthetase
5.    gi|52002332    Mass: 57768    Score: 74     Expect: 0.0086  Matches: 14
 GMP synthetase [Bacillus licheniformis ATCC 14580]
6.    gi|7707810    Mass: 73020    Score: 68     Expect: 0.035  Matches: 14
 penicillin binding protein 3 [Bacillus cereus]
      gi|25528879    Mass: 72966    Score: 67     Expect: 0.037  Matches: 14
 penicillin-binding protein 3 - Bacillus cereus
7.    gi|30020327    Mass: 72828    Score: 66     Expect: 0.05  Matches: 13
 MecA protein [Bacillus cereus ATCC 14579]
      gi|47570167    Mass: 57495    Score: 41     Expect: 16  Matches: 9
 mecA protein [Bacillus cereus G9241]
8.    gi|18145572    Mass: 20100    Score: 52     Expect: 1.4  Matches: 9
 probable cob(I)alamin adenosyltransferase [Clostridium perfringens str. 13]

Search Parameters

Type of search         : Peptide Mass Fingerprint
Enzyme                 : Trypsin
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.1 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 1
Number of queries      : 66

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