Mascot Search Results

User            : 
Email           : 
Search title    : Sequence Query Example (error tolerant tag)
Database        : SwissProt 51.6 (257964 sequences; 93947433 residues)
Timestamp       : 19 Feb 2007 at 14:11:21 GMT



Protein hits    : CASB_BOVIN Beta-casein precursor - Bos taurus (Bovine)


  IF3X_DICDI Putative eukaryotic translation initiation factor 3 subunit (eIF-3) - Dictyostelium discoideum (Sli

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 33 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    CASB_BOVIN    Mass: 25091    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein precursor - Bos taurus (Bovine)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
1   1031.4000   2060.7854   1980.8548   79.9306 0  78  1.7e-006 1  U    K.FQSEEQQQTEDELQDK.I

 
      Proteins matching the same set of peptides:
      CASB_CAPHI    Mass: 24849    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein precursor - Capra hircus (Goat)
      CASB_SHEEP    Mass: 24859    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein precursor - Ovis aries (Sheep)

2.    IF3X_DICDI    Mass: 148825   Score: 78     Matches: 1(1)  Sequences: 1(1)
 Putative eukaryotic translation initiation factor 3 subunit (eIF-3) - Dictyostelium discoideum (Sli
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1031.4000   2060.7854   1798.8949   261.8906 1  78  1.7e-006 1  U    K.LGGTPEEQQKDIEDLK.A

Search Parameters

Type of search         : Sequence Query
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.2 Da
Fragment Mass Tolerance: ± 0.2 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
etag(977.4,[Q|K][Q|K][Q|K]EE,1619.7)
Score greater than 33 indicates identity
Score    Expect     Delta  Hit  Protein     Peptide
 78.1  1.7e-006   79.9306    1   CASB_BOVIN K.FQSEEQQQTEDELQDK.I
 78.1  1.7e-006  261.8906    2   IF3X_DICDI K.LGGTPEEQQKDIEDLK.A