Mascot Search Results

User            : 
Email           : 
Search title    : Peptide Mass Fingerprint Example
Database        : SwissProt 51.6 (257964 sequences; 93947433 residues)
Timestamp       : 19 Feb 2007 at 14:08:10 GMT
Top Score       : 194 for PML_HUMAN, Probable transcription factor PML (Tripartite motif-containing protein 19) (RING finger protein 71)

Mascot Score Histogram

Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 67 are significant (p<0.05).

Score Distribution

Protein Summary Report

Help
  Significance threshold p< Max. number of hits

  

Index

  Accession  Mass  Score  Description
1. PML_HUMAN  97455194  Probable transcription factor PML (Tripartite motif-containing protein 19) (RING finger protein 71)
2. MURC_IDILO  5299451  UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase) - I

Results List

1.    PML_HUMAN    Mass: 97455    Score: 194    Expect: 1e-014  Matches: 15
 Probable transcription factor PML (Tripartite motif-containing protein 19) (RING finger protein 71)
      Observed  Mr(expt)  Mr(calc)   Delta Start  End Miss  Peptide
  814.4300   813.4227   813.4708   -0.0481  319 -  325 0   R.LDAVLQR.I
  958.3500   957.3427   957.4080   -0.0653  373 -  380 0   R.TDGFDEFK.V
  1000.3300   999.3227   999.3967   -0.0740  308 -  315 0   R.DYEEMASR.L
  1165.3900   1164.3827   1164.5081   -0.1253  491 -  500 1   K.MESEEGKEAR.L
  1182.4400   1181.4327   1181.5677   -0.1349  34 -  44 0   R.QPSPSPSPTER.A
  1191.5000   1190.4927   1190.6520   -0.1592  161 -  170 0   K.HEARPLAELR.N
  1300.4700   1299.4627   1299.6419   -0.1792  504 -  515 0   R.SSPEQPRPSTSK.A
  1355.5300   1354.5227   1354.6841   -0.1613  361 -  372 0   R.QEEPQSLQAAVR.T
  1423.5200   1422.5127   1422.6779   -0.1652  45 -  56 0   R.APASEEEFQFLR.C
  1426.5700   1425.5627   1425.7365   -0.1737  516 -  529 0   K.AVSPPHLDGPPSPR.S
  1624.7400   1623.7327   1623.8692   -0.1365  359 -  372 1   R.LRQEEPQSLQAAVR.T
  2265.1100   2264.1027   2264.0292   0.0735  574 -  594 0   R.ELDDSSSESSDLQLEGPSTLR.V
  2544.4100   2543.4027   2543.2908   0.1120  595 -  616 0   R.VLDENLADPQAEDRPLVFFDLK.I
  2653.3900   2652.3827   2652.2780   0.1048  530 -  555 0   R.SPVIGSEVFLPNSNHVASGAGEAEER.V
  2882.5000   2881.4927   2881.3777   0.1150  8 -  33 0   R.SPRPQQDPARPQEPTMPPPETPSEGR.Q
      No match to: 1320.4000, 1348.4100, 2550.3000

2.    MURC_IDILO    Mass: 52994    Score: 51     Expect: 2  Matches: 5
 UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase) - I
      Observed  Mr(expt)  Mr(calc)   Delta Start  End Miss  Peptide
  1191.5000   1190.4927   1190.6407   -0.1480  320 -  330 1   K.SALNKFEGIGR.R
  1355.5300   1354.5227   1354.6551   -0.1324  269 -  280 0   K.QTASESCFTILR.K
  1426.5700   1425.5627   1425.6677   -0.1050  332 -  343 0   R.FQHYGNFAISDK.D
  1624.7400   1623.7327   1623.8468   -0.1141  135 -  150 0   K.TTTTSLVASIFAEAGR.D
  2653.3900   2652.3827   2652.3984   -0.0157  124 -  150 1   R.HGIAVAGTHGKTTTTSLVASIFAEAGR.D
      No match to: 814.4300, 958.3500, 1000.3300, 1165.3900, 1182.4400, 1300.4700, 1320.4000, 1348.4100, 1423.5200, 2265.1100, 2544.4100, 2550.3000, 2882.5000

Search Parameters

Type of search         : Peptide Mass Fingerprint
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.2 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 1
Number of queries      : 18

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