MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Unassigned peptides, 1–100 (out of 26931)

Page: 1 2 3 4 5 6 … 270 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) Delta M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) Delta M Score Expect Rank Peptide
17701 731.5877 1461.1608 1460.7932 0.3677 0 34 0.064 +1Score > 34 indicates identity
Score > 34 indicates homology		 VLPAPMLQYGGR + Oxidation (M)
6333 555.0061 1107.9977 1107.6168 0.3809 0 33 0.053 +1Score > 33 indicates identity
Score > 33 indicates homology		 VSAETWK
9461 603.8887 1205.7629 1205.7086 0.0544 0 32 0.092 +1Score > 34 indicates identity VMIWELK
1256 451.3809 900.7472 900.5514 0.1958 0 32 0.063 +1Score > 33 indicates identity ELQIVR
6550 558.5968 1115.1791 1115.7158 -0.5367 0 32 0.064 +1Score > 33 indicates identity LQLLDVK
966 443.8478 885.6810 885.5518 0.1293 0 32 0.058 +1Score > 32 indicates identity LLGGNIR
6602 559.3689 1116.7232 1116.6634 0.0598 0 32 0.056 +1Score > 32 indicates identity EPLETLK
2360 480.4273 958.8400 958.5569 0.2831 0 32 0.079 +1Score > 33 indicates identity EGELLVR
2613 486.8810 971.7474 971.6623 0.0852 0 32 0.077 +1Score > 33 indicates identity ILITPK
2600 486.3981 970.7817 970.5933 0.1884 0 31 0.079 +1Score > 33 indicates identity IIEPSLR
20756 790.5842 1579.1538 1579.8705 -0.7167 0 31 0.069 +1Score > 32 indicates identity
Score > 32 indicates homology		 SFIQSAHLIQHR
15301 693.4837 1384.9528 1384.9009 0.0519 1 31 0.069 +1Score > 32 indicates identity ALVAVLREVK
7019 566.3740 1130.7334 1130.7154 0.0180 0 31 0.069 +1Score > 32 indicates identity LLVEEIK
3743 508.9142 1015.8138 1016.5373 -0.7234 0 31 0.096 +1Score > 33 indicates identity AVEGEGGVR
3423 502.4663 1002.9181 1002.6317 0.2864 0 31 0.11 +1Score > 34 indicates identity LIDVQK
4346 519.3492 1036.6838 1037.5046 -0.8207 0 31 0.15 +1Score > 35 indicates identity SQMQTSGR
2353 480.3125 958.6104 958.5205 0.0899 0 30 0.06 +1Score > 31 indicates identity ELPTEAR
988 444.3452 886.6759 887.5311 -0.8552 0 30 0.088 +1Score > 32 indicates identity LTQGIGR
18289 742.6085 1483.2024 1482.7445 0.4580 0 30 0.079 +1Score > 32 indicates identity SGIQQIIECFR
1326 453.2982 1356.8729 1356.7422 0.1306 0 30 0.065 +1Score > 35 indicates identity
Score > 31 indicates homology		 SICSMLLIK + Oxidation (M)
8833 593.7386 1185.4626 1185.6661 -0.2036 0 30 0.071 +1Score > 35 indicates identity
Score > 31 indicates homology		 LILMNPEGR
5823 544.9181 1087.8216 1087.6009 0.2208 0 30 0.13 +1Score > 35 indicates identity
Score > 33 indicates homology		 HHPDILGR
2996 494.3508 986.6870 986.5267 0.1603 0 30 0.095 +1Score > 32 indicates identity SQGLPEGR
6967 565.4284 1128.8423 1128.6849 0.1574 1 30 0.1 +1Score > 32 indicates identity SLVQLRNR
8514 588.4987 1762.4742 1762.9570 -0.4828 0 30 0.12 +1Score > 36 indicates identity
Score > 33 indicates homology		 SGAGSHATHELPAIK
15965 704.5205 1407.0265 1407.8217 -0.7952 0 29 0.11 +1Score > 32 indicates identity SLIFDGVLEK
23429 570.4275 1708.2607 1707.8848 0.3758 1 29 0.091 +1Score > 32 indicates identity MDERLLGPPPPGGGR + Oxidation (M)
8814 593.4394 1184.8642 1185.6588 -0.7945 0 29 0.1 +1Score > 32 indicates identity ASQGSLLPGGR
26864 971.6546 1941.2947 1940.7517 0.5430 0 29 0.073 +1Score > 31 indicates identity
Score > 30 indicates homology		 QTFLQDCEDDGETAAGGR + Gln->pyro-Glu (N-term Q)
26543 639.9431 1916.8076 1916.9938 -0.1862 0 29 0.075 +1Score > 31 indicates identity SLEAHLSAGPQLSNGHR
9479 604.0742 1809.2008 1808.8047 0.3961 0 29 0.22 +1Score > 35 indicates identity SPYQVGEGNSNEDNR
964 443.8425 885.6705 885.5630 0.1075 1 29 0.11 +1Score > 32 indicates identity AAVRGLR
2747 488.4920 974.9694 974.6368 0.3326 0 29 0.16 +1Score > 34 indicates identity ITGLGVK
6293 554.3471 1106.6796 1106.7307 -0.0511 0 29 0.092 +1Score > 31 indicates identity VSILLFK
12698 651.9766 1301.9386 1301.7798 0.1587 0 29 0.078 +1Score > 33 indicates identity
Score > 31 indicates homology		 GVSLLPSDVK
4914 529.3385 1056.6625 1057.5063 -0.8438 0 29 0.072 +1Score > 30 indicates identity
Score > 30 indicates homology		 GYYQGGGGR
6039 549.4021 1096.7896 1097.6315 -0.8419 0 29 0.12 +1Score > 35 indicates identity
Score > 32 indicates homology		 PIEVQVGGR
581 431.8358 861.6570 861.5527 0.1043 0 29 0.3 +1Score > 36 indicates identity INLSK
2439 482.3169 962.6192 962.4903 0.1289 0 29 0.11 +1Score > 35 indicates identity
Score > 32 indicates homology		 SGSSTPQR
2556 485.2796 968.5447 968.5027 0.0420 0 29 0.058 +1Score > 33 indicates identity
Score > 29 indicates homology		 MAMNAK + Oxidation (M)
3009 494.4141 986.8137 986.6246 0.1891 0 29 0.14 +1Score > 33 indicates identity ILIDLTR
2702 488.3238 974.6330 974.5518 0.0812 0 29 0.11 +1Score > 32 indicates identity LTIEAER
4150 515.4444 1028.8742 1029.5689 -0.6947 0 29 0.13 +1Score > 33 indicates identity IAGTPGTGGR
4147 515.4328 1028.8511 1029.5689 -0.7178 0 29 0.069 +1Score > 33 indicates identity
Score > 30 indicates homology		 IAGTPGTGGR
3919 511.4145 1020.8145 1021.5097 -0.6952 0 29 0.18 +1Score > 34 indicates identity TNMNLGGR + Oxidation (M)
6844 563.4695 1124.9244 1125.5029 -0.5784 0 29 0.12 +1Score > 32 indicates identity
Score > 32 indicates homology		 SSNCVVDGR
9438 603.4384 1204.8622 1204.5806 0.2816 0 29 0.11 +1Score > 33 indicates identity
Score > 32 indicates homology		 SGQEQIEDR
9919 610.8803 1219.7460 1219.5456 0.2004 0 29 0.082 +1Score > 32 indicates identity
Score > 30 indicates homology		 PVCVPCPGGR
15989 705.0654 1408.1162 1407.6332 0.4831 1 29 0.14 +1Score > 32 indicates identity QTFCFMNRR + Oxidation (M)
7038 566.5409 1131.0673 1130.7154 0.3519 0 28 0.07 +1Score > 33 indicates identity
Score > 29 indicates homology		 LLVEEIK
15738 700.9941 1399.9735 1399.7945 0.1790 0 28 0.072 +1Score > 32 indicates identity
Score > 29 indicates homology		 IYVGNLPPDIR
4195 516.9151 1031.8156 1031.4866 0.3290 0 28 0.089 +1Score > 34 indicates identity
Score > 30 indicates homology		 SGGGSHGSSR
30531 789.5559 2365.6460 2365.2702 0.3758 1 28 0.079 +1Score > 30 indicates identity
Score > 30 indicates homology		 SLSSSSIGSNSTYLTSKSK
900 441.3072 880.5998 880.4932 0.1066 0 28 0.14 +1Score > 32 indicates identity MLEGK + Oxidation (M)
987 444.3397 886.6648 886.4024 0.2625 0 28 0.11 +1Score > 32 indicates identity
Score > 31 indicates homology		 PCAHGGR
14022 672.4632 1342.9118 1343.6704 -0.7586 0 28 0.069 +1Score > 32 indicates identity
Score > 29 indicates homology		 AAATAGPAWDGGR
5142 533.3504 1064.6863 1064.6110 0.0753 0 28 0.056 +1Score > 32 indicates identity
Score > 28 indicates homology		 LAEPYGK
8700 590.9593 1179.9041 1179.6556 0.2486 0 28 0.17 +1Score > 33 indicates identity
Score > 33 indicates homology		 AHGPGALMLK + Acetyl (Protein N-term)
8124 582.9875 1163.9605 1164.6416 -0.6811 0 28 0.19 +1Score > 33 indicates identity MINENLK + Oxidation (M)
10938 626.5226 1251.0305 1250.7469 0.2837 0 28 0.11 +1Score > 33 indicates identity
Score > 31 indicates homology		 STVPLPLAPGR
6570 558.9419 1115.8693 1116.7223 -0.8530 1 28 0.12 +1Score > 33 indicates identity
Score > 31 indicates homology		 SGLLVKGR
13003 656.5087 1311.0029 1310.6444 0.3585 0 28 0.18 +1Score > 35 indicates identity
Score > 33 indicates homology		 IAMQTLDMGR + 2 Oxidation (M)
16437 711.5578 1421.1010 1420.6301 0.4709 0 28 0.084 +1Score > 33 indicates identity
Score > 29 indicates homology		 SGGSGSESDHTTR
889 440.3420 878.6694 878.5503 0.1191 0 28 0.2 +1Score > 33 indicates identity MALIK + Oxidation (M)
8177 583.8920 1165.7694 1165.6739 0.0955 0 28 0.096 +1Score > 32 indicates identity
Score > 30 indicates homology		 AVFWDIK
4939 529.4804 1056.9461 1056.5910 0.3551 1 28 0.11 +1Score > 35 indicates identity
Score > 30 indicates homology		 SGAARAGPAR
1570 459.9295 917.8444 917.5789 0.2655 0 28 0.29 +1Score > 35 indicates identity IQEIK
5547 540.3377 1078.6607 1078.5502 0.1105 1 28 0.12 +1Score > 31 indicates identity
Score > 31 indicates homology		 SGRHGEHR
6892 564.4016 1126.7886 1127.6365 -0.8478 1 28 0.13 +1Score > 35 indicates identity
Score > 31 indicates homology		 SGVRMFK + Oxidation (M)
13772 668.4114 1334.8082 1335.5535 -0.7452 0 28 0.11 +1Score > 31 indicates identity
Score > 30 indicates homology		 MEMCSGSNK + Oxidation (M)
111 408.2971 814.5796 815.4735 -0.8939 0 28 0.12 +1Score > 31 indicates identity LVGNGGR
7234 569.4246 1136.8346 1136.6498 0.1848 0 28 0.2 +1Score > 33 indicates identity SGIILFMGR
17356 725.9996 1449.9847 1450.6745 -0.6898 0 27 0.073 +1Score > 32 indicates identity
Score > 29 indicates homology		 MFHDSLAGGSGGR + Oxidation (M)
4360 519.4195 1036.8244 1036.5831 0.2414 0 27 0.13 +1Score > 33 indicates identity
Score > 31 indicates homology		 SGTPMIK + Oxidation (M)
18363 744.0470 1486.0794 1485.9235 0.1560 1 27 0.13 +1Score > 32 indicates identity
Score > 31 indicates homology		 KGTNIILNIGR
7029 566.4291 1130.8436 1130.7154 0.1281 0 27 0.21 +1Score > 33 indicates identity
Score > 33 indicates homology		 LLVEEIK
5395 537.3973 1072.7801 1072.4664 0.3137 1 27 0.18 +1Score > 33 indicates identity
Score > 32 indicates homology		 GDRYCGGR
243 416.3668 830.7190 831.4321 -0.7130 0 27 0.11 +1Score > 34 indicates identity
Score > 30 indicates homology		 SGGSGPAR
8370 586.4501 1756.3286 1755.8813 0.4473 0 27 0.12 +1Score > 35 indicates identity
Score > 31 indicates homology		 SIVTAEVSSMPACK
7585 574.9766 1147.9386 1147.6002 0.3384 1 27 0.079 +1Score > 33 indicates identity
Score > 29 indicates homology		 GAMGRGGIGGR + Oxidation (M)
8031 581.4041 1160.7937 1161.5084 -0.7147 0 27 0.13 +1Score > 33 indicates identity
Score > 31 indicates homology		 MATASPAADGGR + Acetyl (Protein N-term); Oxidation (M)
10851 625.2110 1248.4074 1247.7441 0.6633 1 27 0.13 +1Score > 35 indicates identity
Score > 31 indicates homology		 IQSERLSK
21843 543.4066 1627.1979 1627.9267 -0.7288 0 27 0.11 +1Score > 31 indicates identity
Score > 30 indicates homology		 SGVLDEPTIATILR
11352 632.5203 1263.0260 1263.6437 -0.6176 0 27 0.22 +1Score > 33 indicates identity SIGSLPCLER
16783 717.5297 1433.0448 1433.8026 -0.7578 1 27 0.2 +1Score > 32 indicates identity SQLDMKHK + Oxidation (M)
4927 529.3983 1056.7821 1056.5910 0.1911 1 27 0.14 +1Score > 32 indicates identity
Score > 31 indicates homology		 SGAARAGPAR
5907 546.5026 1090.9907 1091.6430 -0.6523 0 27 0.25 +1Score > 33 indicates identity SGIESALK
11452 634.3694 1900.0865 1900.9210 -0.8345 0 27 0.22 +1Score > 33 indicates identity MSETAPAETAAPAPVEK + Acetyl (Protein N-term); Oxidation (M)
3746 508.9258 1015.8370 1016.5009 -0.6638 0 27 0.24 +1Score > 33 indicates identity QDSSPSPR
21516 806.5768 1611.1391 1610.8663 0.2728 1 27 0.1 +1Score > 31 indicates identity
Score > 29 indicates homology		 SGMSPEQSKTK
22227 824.6227 1647.2308 1647.8307 -0.5999 1 27 0.28 +1Score > 34 indicates identity NRTLTSSLPCPGGR
26288 633.5032 1897.4878 1898.0859 -0.5981 0 27 0.11 +1Score > 31 indicates identity
Score > 30 indicates homology		 ILSFHNNTSLLLINR
5236 534.3943 1066.7741 1066.6266 0.1475 0 27 0.12 +1Score > 32 indicates identity
Score > 30 indicates homology		 ASVFDLK
15464 696.4803 1390.9460 1390.8210 0.1250 1 27 0.11 +1Score > 32 indicates identity
Score > 30 indicates homology		 MAVARAVLEK + Oxidation (M)
1330 453.3435 904.6724 904.5990 0.0734 0 27 0.097 +1Score > 34 indicates identity
Score > 29 indicates homology		 FIPLK
6352 555.3901 1108.7657 1109.5994 -0.8338 0 27 0.12 +1Score > 32 indicates identity
Score > 30 indicates homology		 SGAIDMTK
11339 632.4744 1262.9342 1262.6710 0.2632 0 27 0.055 +1Score > 33 indicates identity
Score > 27 indicates homology		 DSQEANLAK
12424 648.0882 1294.1619 1294.7771 -0.6152 0 27 0.36 +1Score > 35 indicates identity LDIVFYLLR
9581 605.5713 1209.1280 1208.5941 0.5339 0 27 0.22 +1Score > 33 indicates identity SEAQLMSGAR + Oxidation (M)
128 409.3768 816.7390 816.4939 0.2451 0 27 0.09 +1Score > 34 indicates identity
Score > 29 indicates homology		 LIGTGGR
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