MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)

Taxonomy :

1: (none)

2: Mus. (16,273 sequences)

Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 21–30 (out of 482)


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+21

Accession Score Description
Family member distances as a dendrogram 1 2::CES3_MOUSE 619 Carboxylesterase 3 OS=Mus musculus GN=Ces3 PE=1 SV=1
3 2::ESTN_MOUSE 217 Liver carboxylesterase N OS=Mus musculus GN=Es1 PE=1 SV=3
2 2::EST1_MOUSE 321 Liver carboxylesterase 1 OS=Mus musculus GN=Ces1 PE=2 SV=1
4 2::EST22_MOUSE 175 Liver carboxylesterase 22 OS=Mus musculus GN=Es22 PE=1 SV=1

+22

Accession Score Description
1 2::MTP_MOUSE 571 Microsomal triglyceride transfer protein large subunit OS=Mus musculus GN=Mttp PE=2 SV=2

+23

Accession Score Description
Family member distances as a dendrogram 1 2::CP2DA_MOUSE 571 Cytochrome P450 2D10 OS=Mus musculus GN=Cyp2d10 PE=2 SV=2
2 2::CP2D9_MOUSE 309 Cytochrome P450 2D9 OS=Mus musculus GN=Cyp2d9 PE=1 SV=1
3 2::CP2DQ_MOUSE 258 Cytochrome P450 2D26 OS=Mus musculus GN=Cyp2d26 PE=1 SV=1

+24

Accession Score Description
1 2::RLA2_MOUSE 538 60S acidic ribosomal protein P2 OS=Mus musculus GN=Rplp2 PE=1 SV=3

+25

Accession Score Description
1 2::PDIA4_MOUSE 499 Protein disulfide-isomerase A4 OS=Mus musculus GN=Pdia4 PE=1 SV=2

+26

Accession Score Description
1 2::RL7_MOUSE 497 60S ribosomal protein L7 OS=Mus musculus GN=Rpl7 PE=2 SV=2

+27

Accession Score Description
1 2::NUCL_MOUSE 476 Nucleolin OS=Mus musculus GN=Ncl PE=1 SV=2

+28

Accession Score Description
1 2::RL6_MOUSE 476 60S ribosomal protein L6 OS=Mus musculus GN=Rpl6 PE=1 SV=3

+29

Accession Score Description
1 2::CP2F2_MOUSE 448 Cytochrome P450 2F2 OS=Mus musculus GN=Cyp2f2 PE=2 SV=1

+30

Accession Score Description
1 2::RRBP1_MOUSE 445 Ribosome-binding protein 1 OS=Mus musculus GN=Rrbp1 PE=2 SV=2
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