MASCOT Search Results

Search metadata
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)

Taxonomy :

1: (none)

2: Mus. (16,273 sequences)

Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 241–250 (out of 482)


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+241

Accession Score Description
1 2::COX6C_MOUSE 44 Cytochrome c oxidase subunit 6C OS=Mus musculus GN=Cox6c PE=1 SV=3

+242

Accession Score Description
1 2::RL27_MOUSE 44 60S ribosomal protein L27 OS=Mus musculus GN=Rpl27 PE=2 SV=2

+243

Accession Score Description
1 2::SAR1B_MOUSE 44 GTP-binding protein SAR1b OS=Mus musculus GN=Sar1b PE=1 SV=1

+244

Accession Score Description
1 2::HNRPU_MOUSE 44 Heterogeneous nuclear ribonucleoprotein U OS=Mus musculus GN=Hnrnpu PE=1 SV=1

+245

Accession Score Description
1 2::UD3A2_MOUSE 44 UDP-glucuronosyltransferase 3A2 OS=Mus musculus GN=Ugt3a2 PE=2 SV=2

+246

Accession Score Description
1 2::RENT1_MOUSE 44 Regulator of nonsense transcripts 1 OS=Mus musculus GN=Upf1 PE=1 SV=2

+247

Accession Score Description
1 2::KAD2_MOUSE 44 Adenylate kinase 2, mitochondrial OS=Mus musculus GN=Ak2 PE=1 SV=5

+248

Accession Score Description
1 2::ATLA3_MOUSE 44 Atlastin-3 OS=Mus musculus GN=Atl3 PE=2 SV=1

+249

Accession Score Description
1 2::ECHA_MOUSE 44 Trifunctional enzyme subunit alpha, mitochondrial OS=Mus musculus GN=Hadha PE=1 SV=1

+250

Accession Score Description
1 2::SC11A_MOUSE 43 Signal peptidase complex catalytic subunit SEC11A OS=Mus musculus GN=Sec11a PE=2 SV=1
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