MASCOT Search Results

Search metadata
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)

Taxonomy :

1: (none)

2: Mus. (16,273 sequences)

Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 221–230 (out of 482)


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+221

Accession Score Description
1 2::P20D1_MOUSE 49 Probable carboxypeptidase PM20D1 OS=Mus musculus GN=Pm20d1 PE=2 SV=1

+222

Accession Score Description
1 2::CP4V3_MOUSE 49 Cytochrome P450 4V3 OS=Mus musculus GN=Cyp4v3 PE=1 SV=1

+223

Accession Score Description
1 2::ADT2_MOUSE 49 ADP/ATP translocase 2 OS=Mus musculus GN=Slc25a5 PE=1 SV=3

+224

Accession Score Description
1 2::H2AX_MOUSE 49 Histone H2A.x OS=Mus musculus GN=H2afx PE=1 SV=2

+225

Accession Score Description
1 2::SCAM3_MOUSE 48 Secretory carrier-associated membrane protein 3 OS=Mus musculus GN=Scamp3 PE=1 SV=2

+226

Accession Score Description
1 2::AT2A2_MOUSE 48 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 OS=Mus musculus GN=Atp2a2 PE=1 SV=2

+227

Accession Score Description
1 2::3BHS3_MOUSE 48 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3 OS=Mus musculus GN=Hsd3b3 PE=2 SV=3

+228

Accession Score Description
1 2::NSUN6_MOUSE 48 Putative methyltransferase NSUN6 OS=Mus musculus GN=Nsun6 PE=2 SV=2

+229

Accession Score Description
1 2::ERP29_MOUSE 47 Endoplasmic reticulum resident protein 29 OS=Mus musculus GN=Erp29 PE=1 SV=2

+230

Accession Score Description
1 2::RL32_MOUSE 47 60S ribosomal protein L32 OS=Mus musculus GN=Rpl32 PE=2 SV=2
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