MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)

Taxonomy :

1: (none)

2: Mus. (16,273 sequences)

Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 11–20 (out of 482)


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+11

Accession Score Description
1 2::RLA0_MOUSE 758 60S acidic ribosomal protein P0 OS=Mus musculus GN=Rplp0 PE=1 SV=3

+12

Accession Score Description
Family member distances as a dendrogram 1 2::ACSL1_MOUSE 751 Long-chain-fatty-acid--CoA ligase 1 OS=Mus musculus GN=Acsl1 PE=1 SV=1
2 2::ACSL5_MOUSE 297 Long-chain-fatty-acid--CoA ligase 5 OS=Mus musculus GN=Acsl5 PE=2 SV=1

+13

Accession Score Description
1 2::RL13_MOUSE 748 60S ribosomal protein L13 OS=Mus musculus GN=Rpl13 PE=2 SV=3

+14

Accession Score Description
Family member distances as a dendrogram 1 2::CP2AC_MOUSE 735 Cytochrome P450 2A12 OS=Mus musculus GN=Cyp2a12 PE=1 SV=1
4 2::CP2A5_MOUSE 47 Cytochrome P450 2A5 OS=Mus musculus GN=Cyp2a5 PE=2 SV=1
2 2::CP2BA_MOUSE 364 Cytochrome P450 2B10 OS=Mus musculus GN=Cyp2b10 PE=1 SV=1
3 2::CP2B9_MOUSE 189 Cytochrome P450 2B9 OS=Mus musculus GN=Cyp2b9 PE=2 SV=1

+15

Accession Score Description
1 2::CP3AB_MOUSE 710 Cytochrome P450 3A11 OS=Mus musculus GN=Cyp3a11 PE=1 SV=1

+16

Accession Score Description
Family member distances as a dendrogram 1 2::EST31_MOUSE 690 Liver carboxylesterase 31 OS=Mus musculus GN=Es31 PE=1 SV=2
3 2::PCYOX_MOUSE 74 Prenylcysteine oxidase OS=Mus musculus GN=Pcyox1 PE=1 SV=1
2 2::CLH_MOUSE 443 Clathrin heavy chain 1 OS=Mus musculus GN=Cltc PE=1 SV=3

+17

Accession Score Description
1 2::RL4_MOUSE 682 60S ribosomal protein L4 OS=Mus musculus GN=Rpl4 PE=1 SV=3

+18

Accession Score Description
Family member distances as a dendrogram 1 2::UD2B5_MOUSE 679 UDP-glucuronosyltransferase 2B5 OS=Mus musculus GN=Ugt2b5 PE=2 SV=1
2 2::UD11_MOUSE 446 UDP-glucuronosyltransferase 1-1 OS=Mus musculus GN=Ugt1a1 PE=2 SV=1
3 2::UD16_MOUSE 268 UDP-glucuronosyltransferase 1-6 OS=Mus musculus GN=Ugt1a6 PE=1 SV=1

+19

Accession Score Description
1 2::PDIA3_MOUSE 675 Protein disulfide-isomerase A3 OS=Mus musculus GN=Pdia3 PE=1 SV=2

+20

Accession Score Description
1 2::NCPR_MOUSE 635 NADPH--cytochrome P450 reductase OS=Mus musculus GN=Por PE=1 SV=2
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