MASCOT Search Results

Search metadata
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)

Taxonomy :

1: (none)

2: Mus. (16,273 sequences)

Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 131–140 (out of 482)


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+131

Accession Score Description
1 2::DAD1_MOUSE 108 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=Mus musculus GN=Dad1 PE=2 SV=3

+132

Accession Score Description
1 2::CALU_MOUSE 108 Calumenin OS=Mus musculus GN=Calu PE=1 SV=1

+133

Accession Score Description
1 2::ERP44_MOUSE 105 Endoplasmic reticulum resident protein ERp44 OS=Mus musculus GN=Erp44 PE=1 SV=1

+134

Accession Score Description
1 2::RL18A_MOUSE 104 60S ribosomal protein L18a OS=Mus musculus GN=Rpl18a PE=1 SV=1

+135

Accession Score Description
1 2::HNRPM_MOUSE 104 Heterogeneous nuclear ribonucleoprotein M OS=Mus musculus GN=Hnrnpm PE=1 SV=3

+136

Accession Score Description
1 2::RS20_MOUSE 100 40S ribosomal protein S20 OS=Mus musculus GN=Rps20 PE=1 SV=1

+137

Accession Score Description
1 2::FRIL1_MOUSE 98 Ferritin light chain 1 OS=Mus musculus GN=Ftl1 PE=1 SV=2

+138

Accession Score Description
1 2::APOA2_MOUSE 94 Apolipoprotein A-II OS=Mus musculus GN=Apoa2 PE=1 SV=1

+139

Accession Score Description
1 2::H2B1B_MOUSE 94 Histone H2B type 1-B OS=Mus musculus GN=Hist1h2bb PE=1 SV=3

+140

Accession Score Description
1 2::RB11A_MOUSE 93 Ras-related protein Rab-11A OS=Mus musculus GN=Rab11a PE=1 SV=3
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