MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

-9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3
Score Mass Matches Sequences emPAI
9.1 2::MGST1_MOUSE 833 18595 38 (25) 6 (3) 1.96
Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

-38 peptide matches (12 non-duplicate, 26 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
29   403.8216 805.6286 805.4932 0.1354 0 7 1.9 +7Score > 35 indicates identity
Score > 23 indicates homology		 U R.IFVGAR.I
8736   591.5090 1181.0033 1181.6648 -0.6615 1 8 1.2 +3Score > 33 indicates identity
Score > 21 indicates homology		 U K.FVRTDEK.V
17151   723.3000 2166.8782 2167.0832 -0.2050 1 4 1.4 +4Score > 34 indicates identity
Score > 18 indicates homology		 U K.MMFMSSATAFQRITNK.V + Oxidation (M)
19996 +5 776.4102 1550.8058 1550.7166 0.0893 0 75 3.5e-007 +1Score > 30 indicates identity
Score > 23 indicates homology		 U K.MMFMSSATAFQR.I
20427 +11 784.4878 1566.9611 1566.7115 0.2497 0 70 1.2e-006 +1Score > 31 indicates identity
Score > 23 indicates homology		 U K.MMFMSSATAFQR.I + Oxidation (M)
20435 +3 784.5320 1567.0493 1566.7115 0.3379 0 63 2.5e-006 +1Score > 32 indicates identity
Score > 19 indicates homology		 U K.MMFMSSATAFQR.I + Oxidation (M)
20855 +3 792.4776 1582.9406 1582.7064 0.2342 0 77 5.8e-008 +1Score > 30 indicates identity
Score > 18 indicates homology		 U K.MMFMSSATAFQR.I + 2 Oxidation (M)
20875   792.6052 1583.1958 1582.7064 0.4894 0 29 0.0046 +1Score > 32 indicates identity
Score > 19 indicates homology		 U K.MMFMSSATAFQR.I + 2 Oxidation (M)
21218   800.5427 1599.0708 1598.7013 0.3695 0 10 0.38 +2Score > 32 indicates identity
Score > 18 indicates homology		 U K.MMFMSSATAFQR.I + 3 Oxidation (M)
23044 +1 563.6700 1687.9882 1687.7942 0.1940 0 49 0.00011 +1Score > 30 indicates identity
Score > 22 indicates homology		 U K.VFANPEDCAGFGK.G
23054 +3 845.1180 1688.2215 1687.7942 0.4273 0 92 4.3e-009 +1Score > 32 indicates identity
Score > 21 indicates homology		 U K.VFANPEDCAGFGK.G
30300   778.2910 2331.8512 2331.1353 0.7159 1 31 0.0055 +1Score > 30 indicates identity
Score > 21 indicates homology		 U K.VFANPEDCAGFGKGENAK.K

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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