MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

-8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2
Score Mass Matches Sequences emPAI
8.1 2::ENPL_MOUSE 1015 103744 143 (68) 36 (24) 1.39
Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

-143 peptide matches (41 non-duplicate, 102 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
153 +1 411.3190 820.6235 820.4929 0.1306 0 22 0.48 +1Score > 34 indicates identity
Score > 31 indicates homology		 K.EIFLR.E
1329   453.3321 904.6496 904.5473 0.1023 0 14 1.3 +3Score > 34 indicates identity
Score > 28 indicates homology		 R.EELVK.N
1863 +7 467.8993 933.7841 933.6103 0.1738 0 36 0.038 +1Score > 35 indicates identity U K.VIVTSK.H
3178 +3 497.9682 993.9218 993.5851 0.3367 0 40 0.0099 +1Score > 33 indicates identity U R.FQNVAK.E
3435 +9 502.8361 1003.6577 1003.6270 0.0307 0 51 0.00069 +1Score > 32 indicates identity U K.NLGTIAK.S
3558   505.0975 1008.1804 1007.5929 0.5876 0 19 0.24 +1Score > 35 indicates identity
Score > 25 indicates homology		 U K.TLDMIK.K
3886 +7 510.9297 1019.8449 1019.5522 0.2927 0 36 0.007 +1Score > 34 indicates identity
Score > 27 indicates homology		 U K.TFEINPR.H
4000   512.9451 1023.8756 1023.5878 0.2878 0 21 0.73 +6Score > 33 indicates identity U K.TLDMIK.K + Oxidation (M)
4487   521.8532 1041.6918 1041.5222 0.1696 0 33 0.036 +1Score > 31 indicates identity U K.FDESEK.T
5342 +1 536.4084 1070.8021 1070.6368 0.1654 0 20 0.17 +1Score > 32 indicates identity
Score > 25 indicates homology		 U K.YLNFVK.G
6019 +5 548.9763 1095.9380 1095.6168 0.3213 0 35 0.032 +1Score > 33 indicates identity
Score > 33 indicates homology		 U K.EFGTNIK.L
8488 +3 588.3910 1174.7674 1174.5927 0.1748 0 46 0.0026 +1Score > 32 indicates identity U K.IYFMAGSSR.K
10058 +1 613.3266 1224.6387 1224.6373 0.0014 0 46 0.0015 +1Score > 30 indicates identity U K.FAFQAEVNR.M
10221 +4 615.4818 1843.4237 1842.9719 0.4517 1 20 0.74 +1Score > 35 indicates identity
Score > 32 indicates homology		 U R.SGYLLPDTKAYGDR.I
10351 +3 617.4633 1232.9120 1232.7948 0.1172 0 64 4.6e-005 +1Score > 33 indicates identity U R.GTTITLVLK.E
10947 +7 626.6405 1251.2664 1250.7842 0.4822 0 60 0.0001 +1Score > 33 indicates identity U K.LIINSLYK.N
11265   631.5172 1261.0197 1260.6383 0.3815 0 29 0.061 +1Score > 33 indicates identity
Score > 29 indicates homology		 U K.YNDTFWK.E
11565 +11 635.9375 1269.8604 1269.6808 0.1796 0 64 1e-005 +1Score > 32 indicates identity
Score > 26 indicates homology		 U K.SGTSEFLNK.M
11910 +6 641.4313 1280.8481 1280.7220 0.1261 0 45 0.00051 +1Score > 32 indicates identity
Score > 25 indicates homology		 U R.SGYLLPDTK.A
11984 +3 642.4470 1282.8794 1282.6751 0.2043 0 44 0.00063 +1Score > 32 indicates identity
Score > 24 indicates homology		 U K.LGVIEDHSNR.T
12012 +6 428.6930 1283.0571 1282.6751 0.3819 0 38 0.004 +1Score > 32 indicates identity
Score > 27 indicates homology		 U K.LGVIEDHSNR.T
12389 +1 647.8558 1293.6970 1293.6323 0.0647 0 42 0.0038 +1Score > 30 indicates identity U K.EAESSPFVER.L
12710 +2 652.3993 1302.7840 1302.6700 0.1140 0 57 1.7e-005 +1Score > 31 indicates identity
Score > 21 indicates homology		 U R.GLFDEYGSK.K
12962   655.9793 1309.9440 1309.7410 0.2030 1 0 6.7 +5Score > 32 indicates identity
Score > 21 indicates homology		 U R.HPLIRDMLR.R + Oxidation (M)
13661 +11 666.4785 1330.9424 1330.7731 0.1693 0 55 0.0003 +1Score > 32 indicates identity U K.SILFVPTSAPR.G
20699 +2 789.4650 1576.9154 1576.7977 0.1177 0 75 8.8e-007 +1Score > 30 indicates identity
Score > 27 indicates homology		 U K.DISTNYYASQK.K
21090 +1 798.0656 1594.1167 1593.8468 0.2698 0 52 7.6e-005 +1Score > 32 indicates identity
Score > 23 indicates homology		 U K.EFEPLLNWMK.D
22423 +1 553.0485 1656.1237 1656.8739 -0.7502 0 21 0.05 +1Score > 32 indicates identity
Score > 20 indicates homology		 U K.NLLHVTDTGVGMTR.E
22759 +2 558.6788 1673.0145 1672.8689 0.1456 0 14 1.2 +2Score > 31 indicates identity
Score > 27 indicates homology		 U K.NLLHVTDTGVGMTR.E + Oxidation (M)
25256   606.9443 1817.8111 1817.0520 0.7591 1 5 1.5 +8Score > 31 indicates identity
Score > 19 indicates homology		 U R.MMKLIINSLYK.N + 2 Oxidation (M)
25715   618.4357 1852.2852 1852.9273 -0.6421 0 17 1.4 +2Score > 31 indicates identity
Score > 31 indicates homology		 U K.TVWDWELMNDIK.P + Oxidation (M)
26103   628.7004 1883.0794 1882.9201 0.1593 0 54 0.00013 +1Score > 30 indicates identity
Score > 28 indicates homology		 U R.VFITDDFHDMMPK.Y
26299   634.0050 1898.9931 1898.9151 0.0781 0 42 0.0026 +1Score > 29 indicates identity U R.VFITDDFHDMMPK.Y + Oxidation (M)
26312   634.2522 1899.7348 1898.9151 0.8198 0 34 0.019 +1Score > 31 indicates identity
Score > 29 indicates homology		 U R.VFITDDFHDMMPK.Y + Oxidation (M)
28284   1037.8995 2073.7845 2074.1085 -0.3240 1 9 0.85 +1Score > 31 indicates identity
Score > 21 indicates homology		 U K.EKNLLHVTDTGVGMTR.E + Oxidation (M)
30281 +1 776.8783 2327.6130 2327.2202 0.3927 1 9 1.7 +3Score > 30 indicates identity
Score > 24 indicates homology		 U R.EEEAIQLDGLNASQIRELR.E
30458   785.5147 2353.5222 2353.2353 0.2869 1 13 1 +1Score > 30 indicates identity
Score > 25 indicates homology		 U K.VEKTVWDWELMNDIK.P + Oxidation (M)
31219 +1 824.6633 2470.9680 2471.3304 -0.3624 1 5 16 +3Score > 29 indicates identity U K.SILFVPTSAPRGLFDEYGSK.K
31304   830.4635 2488.3686 2488.1307 0.2379 0 74 1.4e-006 +1Score > 28 indicates identity U R.LTESPCALVASQYGWSGNMER.I
31419   836.8608 2507.5606 2507.2491 0.3114 1 29 0.061 +1Score > 29 indicates identity U K.TETVEEPLEEDEAAKEEK.E
32559 +3 958.2816 2871.8228 2871.4332 0.3897 1 46 0.0011 +1Score > 28 indicates identity U R.TDDEVVQREEEAIQLDGLNASQIR.E

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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