MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

-7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
2::RDH7_MOUSE 1023 38455 60 (45) 15 (12) 2.50
Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2::H17B6_MOUSE 612 38949 32 (23) 11 (7) 1.03
Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

-67 peptide matches (23 non-duplicate, 44 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 Peptide
6856 +1 563.8530 1125.6915 1125.5536 0.1378 0 9 1.4 +3Score > 30 indicates identity
Score > 23 indicates homology		 U X R.TNVSNYER.L
7986 +1 580.4612 1158.9079 1158.6843 0.2237 0 30 0.032 +1Score > 33 indicates identity
Score > 28 indicates homology		 U X R.IVNVSSALGR.V
8704 +2 591.0157 1180.0169 1179.6370 0.3799 0 45 0.0032 +1Score > 33 indicates identity U X X R.QVVSHLQDK.Y + Gln->pyro-Glu (N-term Q)
8793 +5 593.3202 1184.6259 1184.6069 0.0190 0 60 1.4e-005 +1Score > 30 indicates identity
Score > 24 indicates homology		 U X X R.YSAGWDAK  
9053 +2 597.3600 1192.7054 1192.6356 0.0698 0 42 0.00096 +1Score > 31 indicates identity
Score > 24 indicates homology		 U X R.VVNISSSMGR.V
9312 +4 601.9466 1201.8786 1201.6941 0.1845 0 43 0.0024 +1Score > 33 indicates identity
Score > 29 indicates homology		 U X K.VAIIEPGGFR.T
9569 +4 605.4728 1208.9310 1208.6305 0.3005 0 46 0.00029 +1Score > 33 indicates identity
Score > 23 indicates homology		 U X R.VVNISSSMGR.V + Oxidation (M)
10234 +3 615.8366 1229.6586 1229.6900 -0.0314 0 44 0.0015 +1Score > 30 indicates identity
Score > 28 indicates homology		 U X R.EISYFGVK.V
10951   626.8283 1877.4630 1877.0713 0.3917 1 11 0.44 +1Score > 34 indicates identity
Score > 20 indicates homology		 U X X K.TSDRLETVILDVTK.T
11940 +5 641.5011 1280.9876 1280.7076 0.2800 0 68 7.3e-006 +1Score > 33 indicates identity
Score > 29 indicates homology		 U X X R.VLAACLTEK.G
12120 +5 644.4271 1286.8396 1286.7114 0.1282 0 54 0.00024 +1Score > 32 indicates identity
Score > 30 indicates homology		 U X K.NFLDSYIK.A
12223   645.4607 1933.3604 1934.0818 -0.7215 0 7 2.6 +4Score > 35 indicates identity
Score > 23 indicates homology		 U X M.WFYLVTLVGLYHLLR.W + Acetyl (Protein N-term)
14901   687.2714 1372.5283 1371.8068 0.7215 1 1 3.9 +9Score > 32 indicates identity
Score > 20 indicates homology		 U X R.GRIVNVSSALGR.V
15346 +2 694.4422 1386.8698 1386.6901 0.1797 0 67 1.9e-006 +1Score > 31 indicates identity
Score > 23 indicates homology		 U X K.YGVEAFSDSLR.R
15708 +1 700.4366 1398.8587 1398.7265 0.1322 0 24 0.019 +1Score > 31 indicates identity
Score > 19 indicates homology		 U X K.YGVEAFSDVLR.H
15881 +1 703.5043 1404.9940 1405.7582 -0.7642 1 12 0.46 +1Score > 35 indicates identity
Score > 22 indicates homology		 U X R.GRVVNISSSMGR.V
16298   710.0087 1418.0029 1417.8636 0.1393 0 65 3.1e-005 +1Score > 32 indicates identity U X X R.LETVILDVTK.T
16431   711.5272 1421.0398 1421.7531 -0.7133 1 10 0.49 +1Score > 35 indicates identity
Score > 19 indicates homology		 U X R.GRVVNISSSMGR.V + Oxidation (M)
18205 +2 741.0224 1480.0302 1479.7813 0.2489 0 68 1.3e-005 +1Score > 32 indicates identity U X K.LWDQTSSEVK.E
19828   515.4047 1543.1924 1542.7912 0.4011 1 29 0.014 +1Score > 32 indicates identity
Score > 23 indicates homology		 U X K.YGVEAFSDSLRR.E
21683 +1 541.1233 1620.3481 1619.9126 0.4354 0 45 0.00063 +1Score > 32 indicates identity
Score > 26 indicates homology		 U X X K.TESIVAATQWVK.E
21684 +5 811.2202 1620.4259 1619.9126 0.5133 0 75 7.7e-007 +1Score > 32 indicates identity
Score > 26 indicates homology		 U X X K.TESIVAATQWVK.E
30717   798.5387 2392.5944 2392.0708 0.5236 1 0 1 +2Score > 30 indicates identity
Score > 13 indicates homology		 U X K.ESYGQQFFDDFCSTTKR.E

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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