MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

-6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1
Score Mass Matches Sequences emPAI
6.1 2::CP1A2_MOUSE 1048 63034 59 (38) 16 (10) 1.16
Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

-59 peptide matches (23 non-duplicate, 36 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
541   430.3399 858.6652 859.5371 -0.8719 0 7 1.1 +2Score > 33 indicates identity
Score > 21 indicates homology		 U R.TQVPK.G
710   436.2864 1305.8373 1305.6952 0.1422 1 14 0.48 +1Score > 34 indicates identity
Score > 23 indicates homology		 U K.DPFVFRPER.F
4590 +4 523.8686 1045.7227 1045.6375 0.0852 0 46 0.0027 +1Score > 33 indicates identity U R.LAQDALK.S
7720 +5 576.9804 1151.9462 1151.6980 0.2482 0 61 8.2e-005 +1Score > 33 indicates identity U K.VMLFGLGK.R
8245   584.9121 1167.8097 1167.6929 0.1168 0 32 0.0062 +1Score > 32 indicates identity
Score > 22 indicates homology		 U K.VMLFGLGK.R + Oxidation (M)
8848   594.0017 1778.9832 1778.8532 0.1300 0 76 2.2e-007 +1Score > 34 indicates identity
Score > 22 indicates homology		 U K.SMTFNPDSGPVWAAR.R
9361 +8 602.4601 1202.9055 1202.7267 0.1789 0 54 0.0001 +1Score > 33 indicates identity
Score > 27 indicates homology		 U R.YLPNPALK.R
12813   653.6696 1305.3247 1305.6952 -0.3705 1 13 1.1 +2Score > 34 indicates identity
Score > 26 indicates homology		 U K.DPFVFRPER.F
14501   680.5705 1359.1264 1358.7285 0.3979 0 0 2.5 +10Score > 33 indicates identity
Score > 17 indicates homology		 U K.DNGGLIPEEK.I
16077 +4 706.2600 1410.5054 1410.7589 -0.2535 0 19 0.15 +1Score > 35 indicates identity
Score > 23 indicates homology		 U K.IHEELDTVVGR.D
19347 +2 763.0747 1524.1348 1523.8187 0.3160 0 63 4.9e-005 +1Score > 32 indicates identity U R.FLTNNNSAIDK.T
20332 +3 782.4965 1562.9785 1562.8538 0.1247 0 72 5e-006 +1Score > 31 indicates identity U R.LSQQYGDVLQIR.I
21770   813.0690 1624.1235 1623.9075 0.2160 0 54 0.00013 +1Score > 32 indicates identity
Score > 27 indicates homology		 U K.NSIQDITSALFK.H
21774   542.3918 1624.1536 1623.9075 0.2461 0 60 3e-005 +1Score > 32 indicates identity
Score > 27 indicates homology		 U K.NSIQDITSALFK.H
22912   561.4618 1681.3637 1681.8869 -0.5233 1 4 3.7 +10Score > 31 indicates identity
Score > 22 indicates homology		 U K.IHEELDTVVGRDR.Q
22945   842.6308 1683.2469 1682.9559 0.2910 1 28 0.08 +1Score > 32 indicates identity
Score > 30 indicates homology		 U R.KIHEELDTVVGR.D
22950   562.1591 1683.4555 1682.9559 0.4996 1 53 0.00012 +1Score > 32 indicates identity
Score > 27 indicates homology		 U R.KIHEELDTVVGR.D
24592 +2 890.5467 1779.0788 1778.8532 0.2256 0 92 8.7e-009 +1Score > 31 indicates identity
Score > 24 indicates homology		 U K.SMTFNPDSGPVWAAR.R
24891   599.3965 1795.1676 1794.8481 0.3195 0 1 2.2 +7Score > 31 indicates identity
Score > 17 indicates homology		 U K.SMTFNPDSGPVWAAR.R + Oxidation (M)
26131 +5 629.5137 1885.5193 1885.1492 0.3701 0 71 1.5e-006 +1Score > 31 indicates identity
Score > 25 indicates homology		 U R.IGSTPVVVLSGLNTIK.Q
26136 +2 943.8500 1885.6854 1885.1492 0.5362 0 47 0.00029 +1Score > 31 indicates identity
Score > 24 indicates homology		 U R.IGSTPVVVLSGLNTIK.Q
26803   646.0558 1935.1456 1934.9543 0.1913 1 1 1.1 +2Score > 30 indicates identity
Score > 14 indicates homology		 U K.SMTFNPDSGPVWAARR.R
32077 +1 893.9524 2678.8355 2678.2447 0.5907 0 68 7.7e-006 +1Score > 29 indicates identity U K.SFSIASDPTSASSCYLEEHVSK.E

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
2::CP1A1_MOUSE 649 63785 6.1
Cytochrome P450 1A1 OS=Mus musculus GN=Cyp1a1 PE=1 SV=2

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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