MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

-5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2
Cut threshold

Score Mass Matches Sequences emPAI
2::PDIA1_MOUSE 1116 64779 127 (57) 36 (19) 1.91
Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2::TXND5_MOUSE 46 50829 39 (2) 11 (2) 0.15
Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

-165 peptide matches (53 non-duplicate, 112 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 Peptide
204 +1 414.4235 1240.2485 1239.7552 0.4933 1 18 0.18 +1Score > 35 indicates identity
Score > 23 indicates homology		 U X R.TLDGFKK.F
644   433.8945 865.7744 866.5469 -0.7726 0 14 0.35 +1Score > 33 indicates identity
Score > 22 indicates homology		 U X K.VYVAK.V
666   434.4350 866.8553 867.4385 -0.5831 1 7 1.4 +5Score > 32 indicates identity
Score > 21 indicates homology		 U X -.MPPRPGR.L + Acetyl (Protein N-term); Oxidation (M)
686 +1 435.3122 868.6099 868.5262 0.0837 0 24 0.23 +1Score > 30 indicates identity U X K.LSSFK.R
916 +12 441.4806 880.9467 880.5262 0.4205 0 26 0.076 +1Score > 33 indicates identity
Score > 27 indicates homology		 X R.FLEGK.I
1987   470.9050 939.7955 939.5269 0.2686 0 6 1.5 +5Score > 33 indicates identity
Score > 21 indicates homology		 U X K.VDQYK.G
2272 +9 477.3560 952.6974 953.5351 -0.8377 1 12 1.6 +4Score > 32 indicates identity
Score > 27 indicates homology		 U X -.MPPRPGR.L
2287 +1 477.4043 952.7941 953.5426 -0.7484 0 26 0.24 +1Score > 33 indicates identity
Score > 32 indicates homology		 U X K.YQLDK.D
2409 +9 481.8064 961.5983 961.5688 0.0295 1 17 0.32 +1Score > 32 indicates identity
Score > 24 indicates homology		 U X K.AVKDEL.-
2545 +5 484.8194 967.6243 967.5582 0.0661 0 30 0.069 +1Score > 31 indicates identity X R.TLDGFK.K
2564 +2 485.3358 968.6571 968.4910 0.1661 0 9 1.2 +2Score > 32 indicates identity
Score > 22 indicates homology		 U X K.VGEHNGGR.D
2585 +1 485.9625 969.9104 969.5300 0.3804 1 5 1.2 +3Score > 32 indicates identity
Score > 19 indicates homology		 U X -.MPPRPGR.L + Oxidation (M)
3153 +1 497.2694 992.5243 992.4541 0.0702 0 25 0.097 +1Score > 31 indicates identity
Score > 28 indicates homology		 U X K.EECPAVR.L
4320 +3 518.9112 1035.8078 1035.6572 0.1506 0 37 0.024 +1Score > 33 indicates identity U X K.LLDFIK.H
4747 +7 526.3769 1050.7393 1050.6317 0.1076 0 37 0.022 +1Score > 33 indicates identity U X K.IFGGEIK.T
4844 +8 527.9448 1053.8750 1053.5365 0.3384 0 41 0.0019 +1Score > 32 indicates identity
Score > 27 indicates homology		 U X K.FFPASADR.T
5131 +1 533.3116 1064.6087 1064.6474 -0.0387 0 41 0.0021 +1Score > 31 indicates identity
Score > 26 indicates homology		 U X K.DGVVLFK.K
5997   548.4647 1094.9147 1094.5123 0.4024 0 13 0.68 +1Score > 33 indicates identity
Score > 24 indicates homology		 U X K.ITEFCHR.F
7649 +6 575.9399 1149.8653 1149.6637 0.2016 0 52 0.00078 +1Score > 33 indicates identity U X K.ALAPEYAK.R
7952 +2 579.9505 1157.8865 1157.5808 0.3057 0 71 7.5e-006 +1Score > 33 indicates identity
Score > 32 indicates homology		 U X K.SVSDYDGK.L
9598   606.0381 1210.0615 1209.6111 0.4504 0 44 0.0036 +1Score > 33 indicates identity U X R.TVIDYNGER.T
9796   609.0250 1216.0354 1215.7219 0.3134 0 44 0.00026 +1Score > 33 indicates identity
Score > 21 indicates homology		 U X K.VHSFPTLK.F
9882   610.4019 1218.7893 1218.7427 0.0466 0 31 0.081 +1Score > 32 indicates identity U X K.GTVLALTEK.S
10570   620.8200 1239.6255 1239.7552 -0.1298 1 0 3.9 +9Score > 30 indicates identity
Score > 19 indicates homology		 U X R.TLDGFKK.F
10721   623.4330 1244.8514 1244.5950 0.2564 0 10 1.7 +4Score > 32 indicates identity
Score > 24 indicates homology		 U X K.YNSMEDAK.V
11010 +1 628.0115 1254.0085 1253.7627 0.2458 0 53 0.00015 +1Score > 32 indicates identity
Score > 27 indicates homology		 U X R.ILEFFGLK.K
11133 +5 629.9551 1257.8957 1257.7325 0.1632 0 54 0.00011 +1Score > 32 indicates identity
Score > 27 indicates homology		 U X K.QLAPIWDK.L
11265 +1 631.5172 1261.0197 1260.5900 0.4298 0 15 1.5 +2Score > 33 indicates identity
Score > 29 indicates homology		 U X K.YNSMEDAK.V + Oxidation (M)
11432   422.7268 1265.1586 1264.6511 0.5075 1 33 0.055 +1Score > 33 indicates identity U X K.KEECPAVR.L
12262   431.0058 1289.9957 1289.7547 0.2410 1 28 0.086 +1Score > 33 indicates identity
Score > 30 indicates homology		 U X K.LKAEGSEIR.L
13826   669.0460 1336.0774 1336.8444 -0.7670 1 2 3.1 +5Score > 33 indicates identity
Score > 20 indicates homology		 U X K.DGVVLFKK.F
13927 +4 670.4967 1338.9788 1338.7023 0.2765 0 55 0.0003 +1Score > 32 indicates identity U X R.NNFEGEITK.E
14534   681.0875 1360.1604 1360.7918 -0.6314 1 12 2.1 +2Score > 33 indicates identity
Score > 27 indicates homology		 U X K.AEGSEIRLAK.V
14821   685.6485 1369.2825 1368.8737 0.4088 0 33 0.042 +1Score > 32 indicates identity U X K.THILLFLPK.S
17180   482.6954 1445.0645 1444.7796 0.2849 0 42 0.00036 +1Score > 32 indicates identity
Score > 21 indicates homology		 U X R.DLDSLHSFVLR.Q
18255 +2 742.4763 1482.9381 1483.7735 -0.8354 1 13 0.92 +1Score > 31 indicates identity
Score > 25 indicates homology		 U X R.GGEKVGEHNGGR.D
18271 +3 495.3531 1483.0373 1483.7735 -0.7362 1 12 1.2 +3Score > 32 indicates identity
Score > 25 indicates homology		 U X R.GGEKVGEHNGGR.D
18442 +7 745.8950 1489.7754 1489.8020 -0.0266 0 67 9.7e-006 +1Score > 33 indicates identity
Score > 29 indicates homology		 U X R.EADDIVNWLK.K
18550 +4 748.0871 1494.1596 1493.8254 0.3342 0 83 1.4e-007 +1Score > 32 indicates identity
Score > 26 indicates homology		 U X R.LITLEEEMTK.Y
18790 +4 502.3231 1503.9474 1503.7925 0.1549 0 55 2.7e-005 +1Score > 31 indicates identity
Score > 22 indicates homology		 U X K.SNFEEALAAHK.Y
18970 +2 756.0795 1510.1444 1509.8204 0.3241 0 56 5.6e-005 +1Score > 32 indicates identity
Score > 26 indicates homology		 U X R.LITLEEEMTK.Y + Oxidation (M)
20606   525.4257 1573.2551 1573.8344 -0.5793 0 3 1.8 +9Score > 32 indicates identity
Score > 18 indicates homology		 U X R.LQPTWNDLGDK.Y
21184   533.4285 1597.2636 1596.7909 0.4727 0 4 1.1 +2Score > 32 indicates identity
Score > 17 indicates homology		 U X K.MDSTANEVEAVK.V + Oxidation (M)
25578 +3 614.7594 1841.2563 1840.9783 0.2779 1 60 8.3e-006 +1Score > 31 indicates identity
Score > 22 indicates homology		 U X K.YKPESDELTAEK.I
25758   619.8037 1856.3892 1856.0773 0.3119 1 21 0.15 +1Score > 31 indicates identity
Score > 25 indicates homology		 U X K.YQLDKDGVVLFK.K
27020   652.4027 1954.1864 1954.0291 0.1573 0 74 1.7e-007 +1Score > 30 indicates identity
Score > 18 indicates homology		 U X K.VLVGANFEEVAFDEK.K
27147   655.4717 1963.3932 1964.0643 -0.6712 1 4 1.1 +3Score > 31 indicates identity
Score > 17 indicates homology		 U X -.MLSRALLCLALAWAAR.V + Oxidation (M)
27175 +3 983.7341 1965.4537 1965.0243 0.4295 1 4 4.1 +4Score > 31 indicates identity
Score > 23 indicates homology		 U X K.YNSMEDAKVYVAK.V + Oxidation (M)
27181   656.2494 1965.7263 1965.0243 0.7020 1 3 9.5 +4Score > 31 indicates identity
Score > 25 indicates homology		 U X K.YNSMEDAKVYVAK.V + Oxidation (M)
29190   723.4642 2167.3709 2167.1153 0.2556 1 13 1.2 +3Score > 30 indicates identity
Score > 26 indicates homology		 U X K.EYTAGREADDIVNWLK.K
29827   751.5771 2251.7094 2252.2408 -0.5315 0 3 1.1 +2Score > 30 indicates identity
Score > 16 indicates homology		 U X K.HNQLPLVIEFTEQTAPK.I
32666   976.6544 2926.9413 2927.5524 -0.6112 0 8 5.9 +3Score > 28 indicates identity U X K.QFLLAAEAIDDIPFGITSNSGVFSK.Y

2 subsets and intersections (4 subset proteins in total)

Score Mass Subset of
2::ANKR5_MOUSE 40 97678 5.1
Ankyrin repeat domain-containing protein 5 OS=Mus musculus GN=Ankrd5 PE=2 SV=1
2 samesets of 2::ANKR5_MOUSE
2::CS2LA_MOUSE 40 23675
Alpha-S2-casein-like A OS=Mus musculus GN=Csn1s2a PE=1 SV=1
2::CD123_MOUSE 40 42278
Cell division cycle protein 123 homolog OS=Mus musculus GN=Cdc123 PE=2 SV=2
2::TMX3_MOUSE 27 56414 5.1
Protein disulfide-isomerase TMX3 OS=Mus musculus GN=Tmx3 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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