MASCOT Search Results

Search metadata
User :  
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Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

-4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2
Score Mass Matches Sequences emPAI
4.1 2::CYB5_MOUSE 1228 16817 63 (48) 8 (6) 5.00
Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

-63 peptide matches (13 non-duplicate, 50 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U Peptide
4049 +8 513.8603 1025.7060 1025.6001 0.1059 0 36 0.018 +1Score > 31 indicates identity U K.VYDLTK.F
4438   520.9165 1039.8184 1039.5212 0.2972 0 3 1.4 +4Score > 32 indicates identity
Score > 16 indicates homology		 U -.MAGQSDK.D + Oxidation (M)
15955 +1 704.4691 1406.9236 1407.8230 -0.8994 0 11 1.5 +5Score > 32 indicates identity
Score > 25 indicates homology		 U K.STWVILHHK.V
15992   470.4068 1408.1987 1407.8230 0.3756 0 39 0.0073 +1Score > 32 indicates identity
Score > 30 indicates homology		 U K.STWVILHHK.V
18020 +7 738.0084 1474.0022 1473.7959 0.2064 0 74 3.5e-006 +1Score > 32 indicates identity U K.YYTLEEIQK.H
18026   492.3889 1474.1449 1473.7959 0.3491 0 6 2 +9Score > 32 indicates identity
Score > 21 indicates homology		 U K.YYTLEEIQK.H
18934   755.5320 1509.0494 1509.8186 -0.7692 1 3 1 +9Score > 32 indicates identity
Score > 15 indicates homology		 U -.MAGQSDKDVK.Y
20552   787.0462 1572.0778 1571.8065 0.2712 0 76 5.9e-007 +1Score > 32 indicates identity
Score > 26 indicates homology		 U K.TYIIGELHPDDR.S
20563 +7 525.0861 1572.2366 1571.8065 0.4301 0 57 4.6e-006 +1Score > 32 indicates identity
Score > 16 indicates homology		 U K.TYIIGELHPDDR.S
22381 +11 552.6631 1654.9676 1654.8437 0.1240 0 46 0.00035 +1Score > 30 indicates identity
Score > 24 indicates homology		 U K.FLEEHPGGEEVLR.E
22386 +5 828.5314 1655.0483 1654.8437 0.2047 0 62 4.6e-005 +1Score > 31 indicates identity U K.FLEEHPGGEEVLR.E
30413 +1 1175.6897 2349.3647 2349.0227 0.3420 0 100 5e-009 +1Score > 29 indicates identity U R.EQAGGDATENFEDVGHSTDAR.E
30415 +10 784.1341 2349.3805 2349.0227 0.3578 0 104 1.7e-009 +1Score > 29 indicates identity U R.EQAGGDATENFEDVGHSTDAR.E

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
1::CYB5_HUMAN 1017 16486 4.1
CYB5_HUMAN

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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