MASCOT Search Results

Search metadata
User :  
E-mail :  
Search title : iPRG2008 SwissProt Mouse
MS data file : D:\iPRG2008\mgf\merged.mgf
Databases :

1: cRAP 20090731 (111 sequences; 37,180 residues)

2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues)
Taxonomy :

1: (none)

2: Mus. (16,273 sequences)
Timestamp : 5 Mar 2010 at 14:04:48 GMT
Warning : No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin/P
Fixed modifications : iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C)
Variable modifications : Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 0.9 Da
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 33,191

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Decoy search summary
Peptide matches in target in Decoy FDR
  – above identity threshold 2343 96 4.10%
  – above identity or homology threshold 2851 220 7.72%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 482)

Page: 1 2 3 4 5 6 … 49 Next 

+1

Accession Score Description
1 1::TRY1_BOVIN 1597 TRY1_BOVIN

+2

Accession Score Description
Family member distances as a dendrogram 1 2::CP2CT_MOUSE 1307 Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1
4 2::CP239_MOUSE 293 Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1
5 2::CP238_MOUSE 202 Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1
2 2::CP254_MOUSE 535 Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1
6 2::CP270_MOUSE 69 Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2
3 2::CY250_MOUSE 382 Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 2::GRP78_MOUSE 1292 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2 2::HSP7C_MOUSE 353 Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
3 2::HS71L_MOUSE 165 Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4
Cut threshold

Score Mass Matches Sequences emPAI
2::GRP78_MOUSE 1292 81404 92 (55) 38 (22) 2.17
78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3
2::HSP7C_MOUSE 353 78937 55 (23) 25 (9) 0.55
Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1
2::HS71L_MOUSE 165 78552 32 (12) 14 (4) 0.25
Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4

-147 peptide matches (76 non-duplicate, 71 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) Delta M Score Expect Rank U 1 2 3 Peptide
637   433.6601 1297.9584 1297.7243 0.2341 1 21 0.4 +1Score > 36 indicates identity
Score > 30 indicates homology		 U X K.FAEEDKK.L
706   436.2225 1305.6456 1304.7656 0.8801 1 3 6.8 +7Score > 34 indicates identity
Score > 24 indicates homology		 U X X K.ITITNDKGR.L
1967 +3 470.3631 938.7116 938.5833 0.1283 0 27 0.069 +1Score > 32 indicates identity
Score > 28 indicates homology		 U X K.FLPFK.V
2401   481.4061 960.7977 961.5436 -0.7459 0 2 1.2 +4Score > 34 indicates identity
Score > 16 indicates homology		 U X K.NQIGDK.E
2720 +9 488.3756 974.7367 974.6004 0.1363 0 54 0.00056 +1Score > 34 indicates identity U X X X R.LIGDAAK.N
3268   500.0147 1497.0222 1497.7820 -0.7597 1 12 0.35 +1Score > 35 indicates identity
Score > 20 indicates homology		 U X K.EFFNGKEPSR.G
3741 +4 508.9092 1015.8039 1015.6633 0.1406 0 34 0.035 +1Score > 33 indicates identity
Score > 32 indicates homology		 U X K.IQQLVK.E
4163   515.9778 1029.9410 1029.5772 0.3638 0 5 4.4 +8Score > 33 indicates identity
Score > 24 indicates homology		 U X K.VMVSYK.G + Oxidation (M)
4601 +3 523.9779 1045.9413 1045.6375 0.3038 0 33 0.059 +1Score > 34 indicates identity U X R.NTVVPTK.K
4827 +2 527.4637 1052.9129 1052.6110 0.3020 0 35 0.0094 +1Score > 33 indicates identity
Score > 27 indicates homology		 U X K.VQVEYK.G
5031   531.3780 1060.7415 1061.6324 -0.8909 0 9 1.2 +5Score > 33 indicates identity
Score > 22 indicates homology		 X R.NTTIPTK.Q
5657 +1 542.4027 1624.1863 1624.9019 -0.7156 0 10 0.96 +2Score > 35 indicates identity
Score > 22 indicates homology		 U X K.SQIHDIVLVGGSTR.I
5924 +3 546.9979 1091.9813 1091.6430 0.3383 0 41 0.0084 +1Score > 33 indicates identity U X X K.ITITNDK.G
6372   555.4712 1108.9278 1109.5630 -0.6353 0 5 2.1 +8Score > 33 indicates identity
Score > 21 indicates homology		 U X R.MVNDAEK.F + Oxidation (M)
6994 +2 565.8689 1129.7232 1129.6101 0.1131 0 32 0.027 +1Score > 31 indicates identity
Score > 29 indicates homology		 U X R.LTPEEIER.M
7202 +1 568.9193 1135.8241 1136.6879 -0.8638 1 9 6 +5Score > 33 indicates identity
Score > 30 indicates homology		 U X K.STGKANK.I
7254 +1 569.6539 1137.2932 1137.5943 -0.3012 0 11 2.1 +5Score > 33 indicates identity
Score > 27 indicates homology		 U X R.MVQEAEK.Y + Oxidation (M)
7346   571.3183 1140.6220 1140.6121 0.0099 0 2 3.6 +4Score > 32 indicates identity
Score > 21 indicates homology		 U X R.ALSSQHQAR.I
7519 +3 573.9761 1145.9377 1145.6536 0.2841 0 38 0.006 +1Score > 33 indicates identity
Score > 29 indicates homology		 U X R.GTLDPVEK.A
8089 +1 582.4200 1162.8254 1162.6437 0.1817 0 13 7.1 +4Score > 35 indicates identity
Score > 34 indicates homology		 U X K.SAVGDEGLK.D
9021 +1 596.5726 1191.1306 1190.6725 0.4581 0 45 0.0028 +1Score > 33 indicates identity
Score > 32 indicates homology		 U X R.VMEHFIK.L
9122 +1 598.4508 1194.8870 1194.6934 0.1936 1 11 9.1 +8Score > 33 indicates identity U X K.STGKENK.I
9459 +3 603.8705 1205.7264 1205.6747 0.0517 0 61 5.5e-005 +1Score > 31 indicates identity U X K.VLEDSDLK.K
9857 +3 609.9429 1217.8713 1217.6486 0.2227 0 45 0.0038 +1Score > 33 indicates identity U X K.ITITNDQNR.L
9960 +5 611.4441 1220.8737 1220.6865 0.1872 0 60 0.0001 +1Score > 33 indicates identity U X K.VCNPIITK.L
10037 +2 612.8115 1835.4126 1834.8204 0.5923 0 35 0.0074 +1Score > 34 indicates identity
Score > 26 indicates homology		 U X K.STAGDTHLGGEDFDNR.M
10741 +2 623.5183 1245.0220 1245.6210 -0.5990 0 12 1.2 +3Score > 33 indicates identity
Score > 25 indicates homology		 U X R.AATGPTIEEVD.-
11042   628.5200 1882.5381 1882.1042 0.4339 1 4 4.7 +7Score > 34 indicates identity
Score > 23 indicates homology		 U X K.IQKLLQDFFNGK.E
11545   635.4900 1268.9654 1268.6856 0.2799 0 55 0.00056 +1Score > 35 indicates identity U X K.ETAEAYLGK.K
11946 +1 641.5476 1281.0806 1280.7220 0.3586 0 55 0.00015 +1Score > 33 indicates identity
Score > 29 indicates homology		 U X K.EIAEAYLGK.T
12202   645.3600 1933.0581 1933.0659 -0.0077 1 0 4 +10Score > 33 indicates identity
Score > 19 indicates homology		 U X R.QATKDAGTIAGLNVMR.I
14676   683.5034 1364.9923 1365.8015 -0.8093 1 11 2.7 +6Score > 35 indicates identity
Score > 28 indicates homology		 U X R.STMKPVQK.V + Oxidation (M)
14847   686.4382 1370.8619 1371.7228 -0.8609 0 10 0.96 +1Score > 31 indicates identity
Score > 22 indicates homology		 U X X X VEIIANDQGNR  
14954   688.0539 2061.1400 2060.2787 0.8612 1 10 2.5 +2Score > 34 indicates identity
Score > 26 indicates homology		 U X M.MKFTVVAAALLLLGAVR.A
15066   689.4552 1376.8958 1376.7204 0.1755 0 8 2.9 +9Score > 34 indicates identity
Score > 25 indicates homology		 U X K.DAGTIAGLNVMR.I + Oxidation (M)
16341   710.8050 1419.5954 1419.7374 -0.1420 1 3 1 +2Score > 34 indicates identity
Score > 16 indicates homology		 U X R.AMTRDNNLLGR.F + Oxidation (M)
16376   474.3878 1420.1416 1419.7374 0.4042 1 5 2 +10Score > 33 indicates identity
Score > 20 indicates homology		 U X R.AMTRDNNLLGR.F + Oxidation (M)
16424   711.5000 1420.9854 1420.7887 0.1967 1 6 3.8 +4Score > 33 indicates identity
Score > 25 indicates homology		 U X K.INDEDKQK.I
18194   740.5968 1479.1791 1478.8336 0.3455 1 42 0.001 +1Score > 32 indicates identity
Score > 25 indicates homology		 U X K.VYEGERPLTK.D
18197   494.1957 1479.5652 1478.8336 0.7316 1 26 0.014 +1Score > 32 indicates identity
Score > 20 indicates homology		 U X K.VYEGERPLTK.D
19656   769.0064 2303.9974 2303.2630 0.7344 1 39 0.0053 +1Score > 34 indicates identity
Score > 29 indicates homology		 U X K.KVTHAVVTVPAYFNDAQR.Q
20122   778.0962 1554.1778 1553.7825 0.3953 0 5 4.5 +7Score > 33 indicates identity
Score > 24 indicates homology		 U X K.CNEIISWLDK.N
20253   781.0129 2340.0170 2339.2118 0.8051 1 9 1.2 +2Score > 34 indicates identity
Score > 22 indicates homology		 U X K.SFYPEEVSSMVLTKMK.E + 2 Oxidation (M)
20607   525.4567 1573.3482 1572.8547 0.4936 1 8 3 +4Score > 32 indicates identity
Score > 26 indicates homology		 U X R.MVQEAEKYK.A + Oxidation (M)
21317   535.3661 1603.0764 1603.8539 -0.7775 0 6 1.4 +6Score > 32 indicates identity
Score > 20 indicates homology		 U X K.SDIDEIVLVGGSTR.I
21354 +2 803.1397 1604.2648 1603.8337 0.4311 0 63 3.2e-005 +1Score > 32 indicates identity
Score > 31 indicates homology		 U X R.NELESYAYSLK.N
22588   832.5840 1663.1535 1662.9185 0.2350 0 12 1.4 +3Score > 32 indicates identity
Score > 26 indicates homology		 U X K.PYIQVDIGGGQTK.T
22754   558.5383 1672.5930 1671.9231 0.6699 1 28 0.018 +1Score > 32 indicates identity
Score > 23 indicates homology		 U X K.MKETAEAYLGK.K
23250   567.3679 1699.0818 1699.9544 -0.8726 1 6 1.8 +6Score > 31 indicates identity
Score > 21 indicates homology		 U X K.MKEIAEAYLGK.T + Oxidation (M)
23465 +3 855.9392 1709.8639 1709.8746 -0.0108 0 66 2.1e-006 +1Score > 32 indicates identity
Score > 22 indicates homology		 U X R.ITPSYVAFTPEGER.L
23473   571.0578 1710.1517 1709.8746 0.2770 0 57 1.7e-005 +1Score > 31 indicates identity
Score > 22 indicates homology		 U X R.ITPSYVAFTPEGER.L
23594 +2 859.6885 1717.3625 1717.8879 -0.5253 0 77 9.4e-007 +1Score > 31 indicates identity
Score > 29 indicates homology		 U X R.TWNDPSVQQDIK.F
24187 +1 585.8629 1754.5669 1753.9808 0.5861 1 13 3.8 +4Score > 31 indicates identity U X R.QATKDAGTIAGLNVLR.I + Gln->pyro-Glu (N-term Q)
25277   607.4422 1819.3048 1818.8255 0.4793 0 55 3.2e-005 +1Score > 31 indicates identity
Score > 23 indicates homology		 U X K.ATAGDTHLGGEDFDNR.L
25347   609.0556 1824.1449 1823.9946 0.1503 0 56 3.5e-005 +1Score > 31 indicates identity
Score > 24 indicates homology		 U X K.TFAPEEISAMVLTK.M
25351   913.2021 1824.3897 1823.9946 0.3950 0 39 0.0015 +1Score > 31 indicates identity
Score > 24 indicates homology		 U X K.TFAPEEISAMVLTK.M
25994   626.3655 1876.0746 1876.0509 0.0237 1 5 1.2 +4Score > 30 indicates identity
Score > 18 indicates homology		 U X K.KSDIDEIVLVGGSTR.I
26001   939.1771 1876.3396 1876.0509 0.2887 1 27 0.0042 +1Score > 31 indicates identity
Score > 16 indicates homology		 U X K.KSDIDEIVLVGGSTR.I
26376   953.0936 1904.1726 1903.9845 0.1881 0 84 1.3e-007 +1Score > 31 indicates identity
Score > 28 indicates homology		 U X K.SFYPEEVSSMVLTK.M
26946   650.1325 1947.3756 1947.0920 0.2836 0 37 0.013 +1Score > 31 indicates identity
Score > 31 indicates homology		 U X X X R.IINEPTAAAIAYGLDK  
26947   974.7142 1947.4139 1947.0920 0.3218 0 43 0.00059 +1Score > 31 indicates identity
Score > 23 indicates homology		 U X X X R.IINEPTAAAIAYGLDK  
27170 +4 983.6427 1965.2709 1965.0047 0.2662 0 96 2e-009 +1Score > 31 indicates identity
Score > 22 indicates homology		 U X K.NQLTSNPENTVFDAK.R
27181   656.2494 1965.7263 1965.0047 0.7216 0 46 0.00041 +1Score > 31 indicates identity
Score > 25 indicates homology		 U X K.NQLTSNPENTVFDAK.R
27237   658.3820 1972.1242 1972.9777 -0.8535 1 2 2.6 +10Score > 30 indicates identity
Score > 19 indicates homology		 U X R.MVNDAEKFAEEDK.K + Oxidation (M)
27932   1019.2338 2036.4530 2036.1632 0.2899 1 48 0.0011 +1Score > 30 indicates identity U X K.TKPYIQVDIGGGQTK.T
28331 +2 693.4307 2077.2703 2077.1078 0.1625 0 82 1.7e-007 +1Score > 30 indicates identity
Score > 26 indicates homology		 U X K.DNHLLGTFDLTGIPPAPR.G
28422   696.5014 2086.4824 2086.1878 0.2947 1 3 3.5 +9Score > 30 indicates identity
Score > 21 indicates homology		 U X K.SDIDEIVLVGGSTRIPK.I
28599   703.5512 2107.6317 2107.2357 0.3960 1 9 0.76 +1Score > 31 indicates identity
Score > 20 indicates homology		 U X K.SQIHDIVLVGGSTRIPK.I
28606   703.9361 2108.7866 2109.1453 -0.3587 1 1 3.7 +8Score > 30 indicates identity
Score > 19 indicates homology		 U X R.DNNLLGRFDLTGIPPAPR.G
28627   704.2735 2109.7987 2109.0881 0.7107 1 3 1.2 +2Score > 30 indicates identity
Score > 16 indicates homology		 U X K.NQVAMNPTNTVFDAKR.L + Oxidation (M)
28780 +1 708.9330 2123.7773 2124.1306 -0.3533 0 10 3 +6Score > 30 indicates identity
Score > 27 indicates homology		 U X K.SQIFSTASDNQPTVTIK.V
28790 +3 1063.1951 2124.3757 2124.1306 0.2450 0 80 5.5e-007 +1Score > 30 indicates identity
Score > 30 indicates homology		 U X K.SQIFSTASDNQPTVTIK.V
29435   732.5444 2194.6112 2193.9438 0.6675 0 2 1.7 +7Score > 30 indicates identity
Score > 17 indicates homology		 U X K.LYQSGCTGPTCTPGYTPGR.A
29643   741.9807 2222.9202 2223.3091 -0.3889 1 2 0.89 +1Score > 30 indicates identity
Score > 14 indicates homology		 U X -.MMKFTVVAAALLLLGAVR.A + 2 Oxidation (M)
29891 +2 755.2793 2262.8160 2262.1048 0.7112 0 69 3.3e-006 +1Score > 30 indicates identity
Score > 27 indicates homology		 U X K.IEWLESHQDADIEDFK.A
31055   815.4667 2443.3782 2443.2838 0.0944 1 2 30 +8Score > 29 indicates identity U X X R.TTPSYVAFTDTERLIGDAAK.N

2 subsets and intersections (3 subset proteins in total)

Score Mass Subset of
2::HSP72_MOUSE 163 77565 3.1, 3.2
Heat shock-related 70 kDa protein 2 OS=Mus musculus GN=Hspa2 PE=1 SV=1
2::HS71A_MOUSE 140 77039 3.1, 3.2, 3.3
Heat shock 70 kDa protein 1A OS=Mus musculus GN=Hspa1a PE=1 SV=2
1 sameset of 2::HS71A_MOUSE
2::HS71B_MOUSE 140 77136
Heat shock 70 kDa protein 1B OS=Mus musculus GN=Hspa1b PE=1 SV=3

+4

Accession Score Description
1 2::CYB5_MOUSE 1228 Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2

+5

Accession Score Description
Family member distances as a dendrogram 1 2::PDIA1_MOUSE 1116 Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1
2 2::TXND5_MOUSE 46 Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2

+6

Accession Score Description
1 2::CP1A2_MOUSE 1048 Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1

+7

Accession Score Description
Family member distances as a dendrogram 1 2::RDH7_MOUSE 1023 Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1
2 2::H17B6_MOUSE 612 Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1

+8

Accession Score Description
1 2::ENPL_MOUSE 1015 Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2

+9

Accession Score Description
1 2::MGST1_MOUSE 833 Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3

+10

Accession Score Description
1 2::RL7A_MOUSE 771 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2
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