MASCOT Search ResultsMASCOT Search Results| User | : | |
|---|---|---|
| : | ||
| Search title | : | iPRG2008 SwissProt Mouse |
| MS data file | : | D:\iPRG2008\mgf\merged.mgf |
| Databases | : | 1: cRAP 20090731 (111 sequences; 37,180 residues) 2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues) |
| Taxonomy | : | 1: (none) 2: Mus. (16,273 sequences) |
| Timestamp | : | 5 Mar 2010 at 14:04:48 GMT |
| Warning | : | No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin/P |
| Fixed modifications | : | iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C) |
| Variable modifications | : | Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 0.9 Da |
| Fragment mass tolerance | : | ± 0.6 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-TRAP |
| Number of queries | : | 33,191 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
| Peptide matches | in target | in Decoy | FDR |
|---|---|---|---|
| – above identity threshold | 2343 | 96 | 4.10% |
| – above identity or homology threshold | 2851 | 220 | 7.72% |
Decoy results are available in |
|||
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 1::TRY1_BOVIN | 1597 | TRY1_BOVIN |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CP2CT_MOUSE | 1307 | Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1 | |
| 4 | 2::CP239_MOUSE | 293 | Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1 | |
| 5 | 2::CP238_MOUSE | 202 | Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1 | |
| 2 | 2::CP254_MOUSE | 535 | Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1 | |
| 6 | 2::CP270_MOUSE | 69 | Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2 | |
| 3 | 2::CY250_MOUSE | 382 | Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::GRP78_MOUSE | 1292 | 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3 | |
| 2 | 2::HSP7C_MOUSE | 353 | Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1 | |
| 3 | 2::HS71L_MOUSE | 165 | Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CYB5_MOUSE | 1228 | Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::PDIA1_MOUSE | 1116 | Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1 | |
| 2 | 2::TXND5_MOUSE | 46 | Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CP1A2_MOUSE | 1048 | Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::RDH7_MOUSE | 1023 | Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1 | |
| 2 | 2::H17B6_MOUSE | 612 | Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1 | |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::ENPL_MOUSE | 1015 | Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::MGST1_MOUSE | 833 | Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::RL7A_MOUSE | 771 | 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 10.1 | 2::RL7A_MOUSE |
771 | 35860 | 52 (28) | 13 (8) | 1.37 | |
| 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2 | |||||||
52 peptide matches (15 non-duplicate, 37 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | Delta | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | Delta | M | Score | Expect | Rank | U | Peptide |
| 365 |
3 |
422.7962 | 843.5778 | 843.5300 | 0.0479 | 0 | 26 | 0.24 | 1Score > 32 indicates identity |
K.LVEAIR.T | |
| 1164 |
4 |
448.4277 | 894.8409 | 894.5782 | 0.2627 | 0 | 26 | 0.077 | 1Score > 33 indicates identity Score > 27 indicates homology |
U | R.AILYK.R |
| 3559 |
505.2000 | 1008.3854 | 1007.6163 | 0.7691 | 1 | 7 | 5.4 | 10Score > 35 indicates identity Score > 27 indicates homology |
-.MPKGK.K + Oxidation (M) | ||
| 7313 |
8 |
570.5139 | 1139.0132 | 1138.7318 | 0.2815 | 0 | 74 | 3.8e-006 | 1Score > 32 indicates identity |
U | K.VAPAPAVVK.K |
| 8958 |
5 |
595.9374 | 1189.8601 | 1189.7274 | 0.1327 | 0 | 38 | 0.03 | 1Score > 35 indicates identity |
U | R.QTATQLLK.L |
| 10370 |
5 |
617.6116 | 1233.2086 | 1232.7372 | 0.4714 | 0 | 58 | 8.4e-005 | 1Score > 33 indicates identity Score > 30 indicates homology |
U | K.VVNPLFEK.R |
| 12294 |
646.5002 | 1290.9858 | 1290.7509 | 0.2349 | 1 | 6 | 3.1 | 10Score > 33 indicates identity Score > 24 indicates homology |
U | R.PETKQEK.K | |
| 18793 |
3 |
753.0072 | 1503.9997 | 1503.8289 | 0.1708 | 0 | 74 | 3.6e-006 | 1Score > 32 indicates identity |
U | K.NFGIGQDIQPK.R |
| 21241 |
801.4720 | 1600.9294 | 1601.7556 | -0.8262 | 1 | 4 | 1.3 | 3Score > 30 indicates identity Score > 18 indicates homology |
U | R.TNYNDRYDEIR.R | |
| 21295 |
1 |
535.1087 | 1602.3043 | 1601.7556 | 0.5487 | 1 | 37 | 0.0052 | 1Score > 32 indicates identity Score > 27 indicates homology |
U | R.TNYNDRYDEIR.R |
| 21963 |
2 |
545.3844 | 1633.1315 | 1632.9290 | 0.2025 | 0 | 61 | 5.2e-006 | 1Score > 32 indicates identity Score > 21 indicates homology |
U | R.AGVNTVTTLVENK.K |
| 21970 |
2 |
817.6842 | 1633.3537 | 1632.9290 | 0.4247 | 0 | 68 | 1.6e-006 | 1Score > 32 indicates identity Score > 22 indicates homology |
U | R.AGVNTVTTLVENK.K |
| 23287 |
851.5785 | 1701.1425 | 1701.9345 | -0.7920 | 1 | 6 | 0.51 | 1Score > 31 indicates identity Score > 15 indicates homology |
U | K.MGVPYCIIKGK.A + Oxidation (M) | |
| 23531 |
1 |
857.6877 | 1713.3609 | 1712.9332 | 0.4278 | 0 | 53 | 0.00013 | 1Score > 32 indicates identity Score > 26 indicates homology |
U | K.VPPAINQFTQALDR.Q |
| 28520 |
3 |
700.6507 | 2098.9304 | 2098.2142 | 0.7162 | 1 | 50 | 0.00027 | 1Score > 30 indicates identity Score > 27 indicates homology |
U | R.LKVPPAINQFTQALDR.Q |
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