MASCOT Search ResultsMASCOT Search Results| User | : | |
|---|---|---|
| : | ||
| Search title | : | iPRG2008 SwissProt Mouse |
| MS data file | : | D:\iPRG2008\mgf\merged.mgf |
| Databases | : | 1: cRAP 20090731 (111 sequences; 37,180 residues) 2: SwissProt 57.14 (514,789 sequences; 181,163,771 residues) |
| Taxonomy | : | 1: (none) 2: Mus. (16,273 sequences) |
| Timestamp | : | 5 Mar 2010 at 14:04:48 GMT |
| Warning | : | No taxonomy indexes for cRAP, taxonomy 'Mus.' ignored. Searching all entries in cRAP |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin/P |
| Fixed modifications | : | iTRAQ4plex (K), iTRAQ4plex (N-term), Methylthio (C) |
| Variable modifications | : | Acetyl (Protein N-term), Gln->pyro-Glu (N-term Q), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 0.9 Da |
| Fragment mass tolerance | : | ± 0.6 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-TRAP |
| Number of queries | : | 33,191 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 32 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
| Peptide matches | in target | in Decoy | FDR |
|---|---|---|---|
| – above identity threshold | 2343 | 96 | 4.10% |
| – above identity or homology threshold | 2851 | 220 | 7.72% |
Decoy results are available in |
|||
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 1::TRY1_BOVIN | 1597 | TRY1_BOVIN |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 1.1 | 1::TRY1_BOVIN |
1597 | 28266 | 74 (48) | 9 (7) | 2.34 | |
| TRY1_BOVIN | |||||||
74 peptide matches (13 non-duplicate, 61 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | Delta | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | Delta | M | Score | Expect | Rank | U | Peptide |
| 7 |
402.2650 | 802.5154 | 802.4783 | 0.0371 | 0 | 7 | 4.5 | 7Score > 35 indicates identity Score > 26 indicates homology |
U | K.SGIQVR.L | |
| 1117 |
447.2009 | 1338.5810 | 1337.8033 | 0.7777 | 1 | 21 | 0.012 | 1Score > 36 indicates identity Score > 14 indicates homology |
U | K.NKPGVYTK.V | |
| 2158 |
475.2905 | 948.5665 | 948.5110 | 0.0555 | 0 | 8 | 1.7 | 4Score > 34 indicates identity Score > 23 indicates homology |
U | K.SAASLNSR.V | |
| 14350 |
9 |
678.0036 | 1353.9927 | 1353.6876 | 0.3052 | 0 | 73 | 2e-006 | 1Score > 32 indicates identity Score > 29 indicates homology |
U | K.APILSDSSCK.S |
| 17066 |
1 |
481.3459 | 1441.0158 | 1440.7704 | 0.2454 | 0 | 51 | 4.6e-005 | 1Score > 32 indicates identity Score > 20 indicates homology |
U | K.SSGTSYPDVLK.C |
| 17080 |
13 |
721.5418 | 1441.0690 | 1440.7704 | 0.2986 | 0 | 74 | 3.8e-006 | 1Score > 32 indicates identity |
U | K.SSGTSYPDVLK.C |
| 17152 |
7 |
723.3268 | 1444.6391 | 1444.7450 | -0.1060 | 0 | 64 | 1.2e-005 | 1Score > 34 indicates identity Score > 28 indicates homology |
U | K.VCNYVSWIK.Q |
| 23185 |
1 |
848.8673 | 2543.5800 | 2543.1738 | 0.4062 | 0 | 67 | 4.6e-006 | 1Score > 33 indicates identity Score > 26 indicates homology |
U | K.SAYPGQITSNMFCAGYLEGGK.D + Oxidation (M) |
| 24382 |
13 |
884.4994 | 1766.9843 | 1766.9051 | 0.0792 | 0 | 110 | 2.3e-010 | 1Score > 30 indicates identity Score > 27 indicates homology |
U | K.LQGIVSWGSGCAQK.N |
| 24388 |
10 |
590.0432 | 1767.1078 | 1766.9051 | 0.2026 | 0 | 68 | 4.4e-006 | 1Score > 31 indicates identity Score > 27 indicates homology |
U | K.LQGIVSWGSGCAQK.N |
| 31512 |
3 |
843.5123 | 2527.5152 | 2527.1789 | 0.3363 | 0 | 70 | 4.3e-006 | 1Score > 29 indicates identity |
U | K.SAYPGQITSNMFCAGYLEGGK.D |
| 31631 |
4 |
854.6325 | 2560.8756 | 2560.3563 | 0.5193 | 0 | 63 | 2.6e-005 | 1Score > 29 indicates identity |
U | K.SIVHPSYNSNTLNNDIMLIK.L |
| 31702 |
859.5194 | 2575.5364 | 2576.3512 | -0.8147 | 0 | 6 | 11 | 3Score > 29 indicates identity |
U | K.SIVHPSYNSNTLNNDIMLIK.L + Oxidation (M) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| 1::TRYP_PIG |
21 | 26675 | 1.1 | ||
| TRYP_PIG | |||||
| 1 sameset of 1::TRYP_PIG | |||||
| 2::TMPSD_MOUSE |
21 | 63956 | |||
| Transmembrane protease, serine 13 OS=Mus musculus GN=Tmprss13 PE=2 SV=2 | |||||
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CP2CT_MOUSE | 1307 | Cytochrome P450 2C29 OS=Mus musculus GN=Cyp2c29 PE=1 SV=1 | |
| 4 | 2::CP239_MOUSE | 293 | Cytochrome P450 2C39 OS=Mus musculus GN=Cyp2c39 PE=2 SV=1 | |
| 5 | 2::CP238_MOUSE | 202 | Cytochrome P450 2C38 OS=Mus musculus GN=Cyp2c38 PE=2 SV=1 | |
| 2 | 2::CP254_MOUSE | 535 | Cytochrome P450 2C54 OS=Mus musculus GN=Cyp2c54 PE=2 SV=1 | |
| 6 | 2::CP270_MOUSE | 69 | Cytochrome P450 2C70 OS=Mus musculus GN=Cyp2c70 PE=2 SV=2 | |
| 3 | 2::CY250_MOUSE | 382 | Cytochrome P450 2C50 OS=Mus musculus GN=Cyp2c50 PE=1 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::GRP78_MOUSE | 1292 | 78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3 | |
| 2 | 2::HSP7C_MOUSE | 353 | Heat shock cognate 71 kDa protein OS=Mus musculus GN=Hspa8 PE=1 SV=1 | |
| 3 | 2::HS71L_MOUSE | 165 | Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CYB5_MOUSE | 1228 | Cytochrome b5 OS=Mus musculus GN=Cyb5a PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::PDIA1_MOUSE | 1116 | Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1 | |
| 2 | 2::TXND5_MOUSE | 46 | Thioredoxin domain-containing protein 5 OS=Mus musculus GN=Txndc5 PE=1 SV=2 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::CP1A2_MOUSE | 1048 | Cytochrome P450 1A2 OS=Mus musculus GN=Cyp1a2 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::RDH7_MOUSE | 1023 | Retinol dehydrogenase 7 OS=Mus musculus GN=Rdh7 PE=2 SV=1 | |
| 2 | 2::H17B6_MOUSE | 612 | Hydroxysteroid 17-beta dehydrogenase 6 OS=Mus musculus GN=Hsd17b6 PE=2 SV=1 | |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::ENPL_MOUSE | 1015 | Endoplasmin OS=Mus musculus GN=Hsp90b1 PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::MGST1_MOUSE | 833 | Microsomal glutathione S-transferase 1 OS=Mus musculus GN=Mgst1 PE=1 SV=3 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 2::RL7A_MOUSE | 771 | 60S ribosomal protein L7a OS=Mus musculus GN=Rpl7a PE=2 SV=2 |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/