MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3682 465.9709 1859.8545 1859.8683 -7.44 1 1 0.88 +1Score > 22 indicates identity
Score > 13 indicates homology
DENDLPTDAMVRTVER
4517 1146.5754 3436.7044 3436.7838 -23.1 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NPIGALQQDQRLSGGLLGGYLAPSLLAQDLAER + 3 Deamidated (NQ)
2790 500.2277 998.4408 998.4711 -30.3 1 1 2.6 +1Score > 17 indicates identity MMMKITGR + 2 Oxidation (M)
4586 709.3690 3541.8086 3541.9233 -32.4 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LFAPLAFTKTYAMAAAAGLAVTLVPVLMGYWIR + Oxidation (M)
3336 475.5491 1423.6255 1423.6878 -43.8 1 1 3.6 +1Score > 19 indicates identity MGEVAYQRAVQR + Oxidation (M); Deamidated (NQ)
3424 756.3894 1510.7642 1510.7086 36.8 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
YVMRITGDDQEGK
4132 810.0735 2427.1987 2427.2104 -4.81 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
YYQNNVAATLSLLQAMVNAGIR + Oxidation (M); 2 Deamidated (NQ)
3676 618.0046 1850.9920 1851.0036 -6.29 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
MNLPPRQQQILELVR + Oxidation (M); Deamidated (NQ)
3889 1122.0724 2242.1302 2242.1205 4.37 1 1 0.88 +1Score > 23 indicates identity
Score > 13 indicates homology
PRFAANLSMLFTEQDFLAR + Oxidation (M)
4532 1150.2622 3447.7648 3447.8119 -13.7 1 1 0.87 +1Score > 21 indicates identity
Score > 13 indicates homology
DVAFGVTVAGRPVSMPQMQRTVGLFINSVALR + 2 Oxidation (M)
3920 1134.5862 2267.1578 2267.1633 -2.42 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QVGFIQKAVQAYMPLHHAGR + Oxidation (M); Deamidated (NQ)
4060 1181.1376 2360.2606 2360.2351 10.8 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QQGQIWMLWPKGQPLPEVPK + Deamidated (NQ)
2594 420.7385 839.4624 839.4865 -28.6 0 1 3.2 +1Score > 18 indicates identity AELALARP
4090 796.0608 2385.1606 2385.2176 -23.9 1 1 0.87 +1Score > 23 indicates identity
Score > 13 indicates homology
IEQNLEVLAVTESWDNGKAIR + Deamidated (NQ)
4582 707.9736 3534.8316 3534.6700 45.7 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
AFYEAPPQGFCEHPLLEPRGCLYVASLEQR + Deamidated (NQ)
2401 665.3260 664.3187 664.3326 -20.9 1 1 3.8 +1Score > 19 indicates identity MNKTR + Oxidation (M)
4502 1143.2394 3426.6964 3426.6476 14.2 0 1 0.9 +1Score > 21 indicates identity
Score > 13 indicates homology
EPNIVGMGGIFVPSSGIVNYAQVTAAMAAEFQR + 3 Deamidated (NQ)
4516 860.1829 3436.7025 3436.6886 4.06 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
TTSPTTTDVANLFIVKDLSAISNNIWGNDAQK + 3 Deamidated (NQ)
2441 696.4413 695.4340 695.4078 37.7 1 1 0.85 +1Score > 13 indicates identity AAAKHAK
4166 818.4113 2452.2121 2452.1300 33.5 1 1 0.9 +1Score > 23 indicates identity
Score > 13 indicates homology
RPGGAGQGQGQGNGQRSNAGGQGQAR + 2 Deamidated (NQ)
4739 1113.8236 4451.2653 4451.4551 -42.6 1 1 3.9 +1Score > 19 indicates identity AIKLAEIVIMAIGALGFVTNHLALMLVALFGMGTHSALFGPVK + Oxidation (M); Deamidated (NQ)
4442 676.1566 3375.7466 3375.6546 27.2 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LMLGMNALVINTGTHLVLVDTGMGQSMGELSR + Oxidation (M); 2 Deamidated (NQ)
3602 353.3824 1761.8756 1761.8971 -12.2 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
EPAMLFALLASGELER + Oxidation (M)
4389 987.8153 2960.4241 2960.5454 -41.0 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
AQVGALAVGGAEKILGATIDQNAHAELVNK + 3 Deamidated (NQ)
3923 1136.0339 2270.0532 2270.0347 8.16 0 1 0.98 +1Score > 22 indicates identity
Score > 13 indicates homology
LMALQQAWNDFGNEMISLR + 2 Oxidation (M); 2 Deamidated (NQ)
4305 665.5765 2658.2769 2658.2054 26.9 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
MFNTKETYPNQTLDNDLCQAVR + Deamidated (NQ)
4314 888.4175 2662.2307 2662.2962 -24.6 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
SWQVRHDFNFATVGVPGLTLMNR + Oxidation (M); 2 Deamidated (NQ)
3270 681.3585 1360.7024 1360.6809 15.8 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
RMVNDYLQPPK + Deamidated (NQ)
3504 828.4490 1654.8834 1654.8600 14.2 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
AGDVIPQVMQVVLER + 2 Deamidated (NQ)
3921 756.7286 2267.1640 2267.1077 24.8 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QQQLMVALDNRGHAAQDSIR + Oxidation (M); Deamidated (NQ)
4067 592.5664 2366.2365 2366.2160 8.66 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
MAKIGFIGTGIMGKPMAQNLQK + 2 Oxidation (M); Deamidated (NQ)
4176 820.7803 2459.3191 2459.2655 21.8 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LSNVLASEQLPPHQARAVIEER + 3 Deamidated (NQ)
3443 512.6284 1534.8634 1534.7926 46.1 1 1 0.94 +1Score > 21 indicates identity
Score > 13 indicates homology
ALLAGEADIGFMRR + Oxidation (M)
4213 830.0860 2487.2362 2487.2506 -5.81 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VEGLWQVELQHADGSLQTIHAR + Deamidated (NQ)
3158 630.8481 1259.6816 1259.6470 27.5 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
EGSAPLQSTSRK
3978 576.2576 2301.0013 2301.0185 -7.49 1 1 4.2 +1Score > 19 indicates identity KNYNVQNDVQQYVDEWTR + 3 Deamidated (NQ)
4665 747.4009 3731.9681 3731.8617 28.5 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
AFKAGACDFIEKPYNDQLLLDSVQAALEHAGLAR + Deamidated (NQ)
3480 796.8504 1591.6862 1591.7487 -39.2 0 1 4.2 +1Score > 19 indicates identity MVTGWGQSMEPTIR
3862 740.6948 2219.0626 2219.0818 -8.67 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
TNQAQVNDARLNLDFTQIR + 3 Deamidated (NQ)
3763 686.7313 2057.1721 2057.1422 14.5 1 1 2.7 +1Score > 17 indicates identity VYAPVGTHETLLAYLVRR
3124 414.5168 1240.5286 1240.5837 -44.4 0 1 3.2 +1Score > 18 indicates identity GWPDDGIAPGTR
3909 754.0525 2259.1357 2259.0866 21.7 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
DTEKLLADTANLAGDQADELR + Deamidated (NQ)
4375 710.6382 2838.5237 2838.4545 24.4 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LVTGQNPQSPKAVAEAVMAVLSGDNVAR + Oxidation (M); Deamidated (NQ)
2990 559.3239 1116.6332 1116.5961 33.3 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LGMALQAQLR + Oxidation (M); Deamidated (NQ)
2858 518.2416 1034.4686 1034.4702 -1.53 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
QMQSNLGQK + 2 Deamidated (NQ)
3592 584.9821 1751.9245 1751.8883 20.7 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
DQGLLALSFPYPQFR + Deamidated (NQ)
3605 882.4447 1762.8748 1762.8638 6.26 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LTFQREQQLWQER + 2 Deamidated (NQ)
3675 926.4938 1850.9730 1850.9097 34.2 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
AFRSMHNLLSEVFQR + Oxidation (M); Deamidated (NQ)
4535 690.7625 3448.7761 3448.7759 0.061 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
MAVDISHLTIATPVAVSSTNPVALELNGAEAIAR + Oxidation (M); 2 Deamidated (NQ)
3194 641.3091 1280.6036 1280.6360 -25.3 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
EYEKLVNGSSR
2440 348.7242 695.4338 695.4078 37.4 0 0 0.91 +1Score > 13 indicates identity LAHSLR
3059 396.5547 1186.6423 1186.6387 3.05 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
GLEPFFPPGVK
4349 676.0854 2700.3125 2700.3256 -4.84 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
TPQAGALTFAGQHLTYAELDQQANR
4148 814.7565 2441.2477 2441.1724 30.8 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
WVTASTWNAVINHSQSQLNQR + 2 Deamidated (NQ)
4081 792.0435 2373.1087 2373.1668 -24.5 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VGCIGVVGADGMQAAVNAVAEVLR + Oxidation (M); 2 Deamidated (NQ)
3832 731.3787 2191.1143 2191.0449 31.7 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QVFNVQGMTCGHCVKAVTR
4728 1106.3153 4421.2321 4421.1375 21.4 1 0 0.93 +1Score > 20 indicates identity
Score > 13 indicates homology
TITQTLQRFYMATSLLINSGQNSLSAEALEDLCVMMAQR + Oxidation (M); Deamidated (NQ)
4493 684.9546 3419.7366 3419.8049 -20.0 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LAAERETLSVVADQFGAPTGADLIADVTAHILR
2983 557.7675 1113.5204 1113.5046 14.3 1 0 0.97 +1Score > 20 indicates identity
Score > 13 indicates homology
TMVMSEKEK + 2 Oxidation (M)
3690 373.3918 1861.9226 1861.8563 35.6 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
MAAVMVGQFHARDAEGR + Oxidation (M); Deamidated (NQ)
4122 804.4008 2410.1806 2410.1548 10.7 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
MQYNRPLFLGNESKPMIEPK + Oxidation (M); 3 Deamidated (NQ)
3437 509.9458 1526.8156 1526.7953 13.2 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
RVNHVAIYLGNDR + Deamidated (NQ)
4567 704.9742 3519.8346 3519.7308 29.5 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
3274 682.8837 1363.7528 1363.8075 -40.1 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
LNVVTLELPPLR + Deamidated (NQ)
3566 576.6093 1726.8061 1726.8461 -23.2 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ALYDRLMAYQSQPR + Oxidation (M)
3664 615.9639 1844.8699 1844.8752 -2.87 0 0 0.95 +1Score > 22 indicates identity
Score > 13 indicates homology
SQISVGDNAEVVENNLR + 2 Deamidated (NQ)
2236 591.3146 590.3073 590.2846 38.5 0 0 0.97 +1Score > 22 indicates identity
Score > 13 indicates homology
VEGMR
3622 600.0088 1797.0046 1796.9533 28.5 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
LAPDQALHHDALVQLR + Deamidated (NQ)
3700 626.6373 1876.8901 1876.9101 -10.7 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ANPAEGKGAMQGVSYIQR + Deamidated (NQ)
4070 790.0488 2367.1246 2367.1311 -2.76 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
MGNGAMDVVDNQLRVHGIPGLR + Oxidation (M); 3 Deamidated (NQ)
3247 444.8768 1331.6086 1331.6503 -31.3 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KSPMLEQGAAER + Oxidation (M)
3510 558.2853 1671.8341 1671.8403 -3.70 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
FNLAVSAHADVLMQR + Deamidated (NQ)
3684 466.2215 1860.8569 1860.8853 -15.3 0 0 0.97 +1Score > 21 indicates identity
Score > 13 indicates homology
NDQSWLLSQTASLPSGR + 2 Deamidated (NQ)
4353 678.5861 2710.3153 2710.3306 -5.63 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
SNLSVGMPLDLLVYRNNSLESMTR + 2 Deamidated (NQ)
4491 855.6942 3418.7477 3418.6245 36.0 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
STFIVGFPGETEEDFQYLLDWLTEAQLDR
3559 862.4576 1722.9006 1722.9529 -30.3 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
FAAERHSALITGVPVR
3604 588.6313 1762.8721 1762.8751 -1.70 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
NDWVVAHDLNPQKAR + Deamidated (NQ)
3918 1134.5847 2267.1548 2267.1633 -3.74 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QVGFIQKAVQAYMPLHHAGR + Oxidation (M); Deamidated (NQ)
3174 423.1997 1266.5773 1266.5914 -11.2 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DVTEGLQAFMR + Deamidated (NQ)
3222 650.3574 1298.7002 1298.6540 35.6 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
HVQVAEMVIEK + Oxidation (M); Deamidated (NQ)
3574 579.9812 1736.9218 1736.8945 15.7 1 0 0.96 +1Score > 22 indicates identity
Score > 13 indicates homology
GGKVQEVEANVVADPPK + Deamidated (NQ)
3565 575.9848 1724.9326 1724.9607 -16.3 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VAGGQLLLANQAGMLLR + Deamidated (NQ)
4609 1193.6123 3577.8151 3577.7812 9.47 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
MAISVLTAFWAVSMLFVITPGADWAYAISAGMR + 2 Oxidation (M)
3956 1143.5194 2285.0242 2285.1184 -41.2 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
MAISIGSKECPYESAIQVFR
3440 384.7023 1534.7801 1534.7735 4.31 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LMPVMAVSNSTLQK + Oxidation (M); Deamidated (NQ)
3815 540.3032 2157.1837 2157.0987 39.4 1 0 4.8 +1Score > 20 indicates identity IPCGLDSVKEQELIDLTAR + Deamidated (NQ)
3942 457.4521 2282.2241 2282.2304 -2.77 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LLGEVCDLVIVVGGDGSLLGAAR
4660 619.8220 3712.8883 3712.8368 13.9 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ALFDVYLMMLEDAALGGEVTEVIKTGQWAQGALR + Oxidation (M); 2 Deamidated (NQ)
3138 417.2368 1248.6886 1248.6285 48.1 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
GKDMLPAFAQR + Oxidation (M)
2857 518.2332 1034.4518 1034.4815 -28.7 0 0 4.3 +1Score > 19 indicates identity MNLGDVSQR + Oxidation (M)
4084 793.3876 2377.1410 2377.2576 -49.1 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NLLLSSYGRQMALIGPNAGFVR + Deamidated (NQ)
4570 587.6470 3519.8383 3519.7308 30.6 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
3660 614.3156 1839.9250 1840.0054 -43.7 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
EKSAQQLNEQLLGLLR + Deamidated (NQ)
3243 441.5561 1321.6465 1321.6734 -20.4 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VMSVALMVVDAR + 2 Oxidation (M)
3594 877.4702 1752.9258 1752.8723 30.6 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
DQGLLALSFPYPQFR + 2 Deamidated (NQ)
3790 704.3605 2110.0597 2110.1105 -24.1 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
TRFLAAVSHDLMQPLNAAR
4501 857.4376 3425.7213 3425.6033 34.4 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
AVVWAHNSHIGNAAATEMGWKGQFNIGQLCR + 3 Deamidated (NQ)
4485 1138.5758 3412.7056 3412.7343 -8.43 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAK + 2 Deamidated (NQ)
2847 515.2194 1028.4242 1028.4597 -34.4 1 0 1.6 +1Score > 15 indicates identity QYQAMRSK + Oxidation (M); 2 Deamidated (NQ)
3439 511.5933 1531.7581 1531.7195 25.2 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LADLFDQYQVYR + 2 Deamidated (NQ)
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