MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2398 332.6751 663.3356 663.3340 2.48 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LFNNR + Deamidated (NQ)
2578 412.7303 823.4460 823.4776 -38.4 1 2 2.9 +1Score > 19 indicates identity RPGRNPK
3983 1152.6205 2303.2264 2303.2130 5.85 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
RTLIAFCLLALCLPATADQR + Deamidated (NQ)
3892 749.0595 2244.1567 2244.0555 45.1 0 2 0.9 +1Score > 22 indicates identity
Score > 14 indicates homology
GELFGPGNAQLPAPNMLMVDR + Oxidation (M); 2 Deamidated (NQ)
3245 443.2247 1326.6523 1326.6667 -10.9 0 2 0.72 +1Score > 21 indicates identity
Score > 13 indicates homology
LPQDSEDPVLSK
3508 554.6445 1660.9117 1660.9009 6.48 0 2 0.83 +1Score > 21 indicates identity
Score > 13 indicates homology
VVLVNTSHPGNIGGAAR
2588 419.1869 836.3592 836.3698 -12.6 0 2 2.4 +1Score > 18 indicates identity MSDTLNR + Deamidated (NQ)
3617 891.4810 1780.9474 1780.9002 26.5 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TILANQLACGHVRGGGR + 2 Deamidated (NQ)
4678 630.1609 3774.9217 3774.8154 28.2 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GGQVFDTLELGMIAAARGYGISMGDLLMVAEDVAQK + 3 Oxidation (M); Deamidated (NQ)
3916 454.2473 2266.2001 2266.1263 32.6 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GVDHVIEVGGPATLEQSMIAAR + Oxidation (M); Deamidated (NQ)
2306 313.6904 625.3662 625.3911 -39.8 0 2 1.2 +1Score > 15 indicates identity GLPALR
2695 471.7674 941.5202 941.5294 -9.74 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LAVDIQQR
3685 931.4648 1860.9150 1860.9469 -17.1 0 2 0.95 +1Score > 22 indicates identity
Score > 14 indicates homology
LINEPYVTISDNLNVR + 2 Deamidated (NQ)
4056 786.7565 2357.2477 2357.2737 -11.0 1 2 0.83 +1Score > 23 indicates identity
Score > 13 indicates homology
MENVIVITGGSRGIGAATALLAAR + Oxidation (M); Deamidated (NQ)
4217 831.3932 2491.1578 2491.1655 -3.12 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
IIAFSRAYNSQTDDNFPEEFK
2609 845.3761 844.3688 844.3861 -20.5 0 2 1.8 +1Score > 17 indicates identity AMDPQQR
4123 804.4096 2410.2070 2410.1144 38.4 1 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology
ILQMLDSTEQRHMSAEDVYK + Oxidation (M); Deamidated (NQ)
3986 1152.6259 2303.2372 2303.2130 10.5 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
RTLIAFCLLALCLPATADQR + Deamidated (NQ)
3203 643.3021 1284.5896 1284.5955 -4.54 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MRFSTEPVMR + 2 Oxidation (M)
4323 669.3237 2673.2657 2673.2163 18.5 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MFNTKETYPNQTLDNDLCQAVR + Oxidation (M)
2734 485.7244 969.4342 969.4259 8.58 0 2 2.8 +1Score > 19 indicates identity MSTESMIR + Oxidation (M)
2482 725.4574 724.4501 724.4595 -13.0 0 2 0.7 +1Score > 13 indicates identity LQPIVR
3493 545.6307 1633.8703 1633.8311 24.0 1 2 0.75 +1Score > 22 indicates identity
Score > 13 indicates homology
RGDNTLNLLPTEYK + Deamidated (NQ)
3787 422.0161 2105.0441 2105.0649 -9.88 1 2 0.99 +1Score > 23 indicates identity
Score > 14 indicates homology
VRNEMGLTNVEIMVPFVR + 2 Deamidated (NQ)
3200 321.3982 1281.5637 1281.5560 5.97 0 2 3.2 +1Score > 19 indicates identity DIYGHAQCYR
4633 613.5037 3674.9785 3674.8160 44.2 1 2 3.4 +1Score > 19 indicates identity MSVECFVTGGSGFVGQHLLAGLTAQGHKTWVLMR + Deamidated (NQ)
3829 730.0487 2187.1243 2187.1205 1.73 1 1 0.8 +1Score > 23 indicates identity
Score > 13 indicates homology
LEGMILEGTLTSGQRLPAER + Oxidation (M); Deamidated (NQ)
3985 1152.6255 2303.2364 2303.2130 10.2 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
RTLIAFCLLALCLPATADQR + Deamidated (NQ)
2610 423.1926 844.3706 844.3675 3.74 0 1 2 +1Score > 17 indicates identity NGGPNQTR + 2 Deamidated (NQ)
3151 419.5273 1255.5601 1255.5945 -27.4 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
YNRDNAFVTR + Deamidated (NQ)
4100 797.4232 2389.2478 2389.2853 -15.7 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LLVLDSQAAKESTGHGSPLPQLK + Deamidated (NQ)
3459 781.8721 1561.7296 1561.7372 -4.85 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VEAFAGNDSIAPQSR + Deamidated (NQ)
4129 809.7432 2426.2078 2426.1383 28.6 1 1 0.77 +1Score > 23 indicates identity
Score > 13 indicates homology
MTQTSPIATDEPHLQRNLTNR + 4 Deamidated (NQ)
2720 319.5163 955.5271 955.5450 -18.8 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NIAKAAPGSK
3707 471.2659 1881.0345 1881.0360 -0.78 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
IDEIIVFHALGKQEIR + Deamidated (NQ)
3955 458.0116 2285.0216 2284.9697 22.7 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
ATHGFAIACMATECDMQLSR + Oxidation (M)
4183 821.7558 2462.2456 2462.2111 14.0 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LPSIGGVNLSQMGNAQGAAEKQFK + Oxidation (M); 2 Deamidated (NQ)
2052 473.2844 472.2771 472.2758 2.86 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
AVAGR
3046 1174.6193 1173.6120 1173.6441 -27.3 1 1 0.79 +1Score > 23 indicates identity
Score > 13 indicates homology
QGFVRLPAMR
3570 577.6453 1729.9141 1729.8280 49.8 0 1 0.84 +1Score > 23 indicates identity
Score > 13 indicates homology
HMNISPNELFMELR
4490 684.7554 3418.7406 3418.6649 22.2 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
TALMLAAMFNRSEILDYLLAQGANPAHQDAR + Oxidation (M); 2 Deamidated (NQ)
3732 655.3553 1963.0441 1962.9720 36.7 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
ALDGLFEMIGKQEQNIR + 2 Deamidated (NQ)
4214 622.8345 2487.3089 2487.2275 32.7 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
TVIMPNGSLSNGIITNTNRQPTR + 4 Deamidated (NQ)
4689 635.0138 3804.0391 3803.8961 37.6 1 1 2.8 +1Score > 18 indicates identity NEMGLTNVEIMVPFVRTLGEASQVVDLLAENGLAR + Oxidation (M); 3 Deamidated (NQ)
2551 404.2071 806.3996 806.3956 5.00 0 1 0.83 +1Score > 23 indicates identity
Score > 13 indicates homology
MTAELAR + Oxidation (M)
3234 657.2734 1312.5322 1312.5969 -49.2 0 1 1.3 +1Score > 15 indicates identity NDMYLTSEAIR + Deamidated (NQ)
4207 622.3107 2485.2137 2485.2244 -4.32 1 1 0.99 +1Score > 23 indicates identity
Score > 14 indicates homology
HQLLMHPQDLQQRQLQDGQR + Oxidation (M); Deamidated (NQ)
3030 575.3550 1148.6954 1148.6917 3.23 0 1 0.88 +1Score > 13 indicates identity AGVPVEPLVLR
3822 1085.5254 2169.0362 2168.9936 19.7 1 1 0.93 +1Score > 22 indicates identity
Score > 14 indicates homology
DYAPKAPNSPGMLDESYGLK + Oxidation (M); Deamidated (NQ)
4697 635.0192 3804.0715 3803.9125 41.8 1 1 2.3 +1Score > 17 indicates identity ERLHQVLQIADHEMNSLHQQLSGAGHAVLLTDAR + Oxidation (M); Deamidated (NQ)
3155 420.5422 1258.6048 1258.6088 -3.18 1 1 0.87 +1Score > 22 indicates identity
Score > 13 indicates homology
MRNNQPITQR + 2 Deamidated (NQ)
4065 592.0396 2364.1293 2364.1817 -22.2 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
ADINLMVVFEALMQERNLTR + 2 Deamidated (NQ)
3795 531.2610 2121.0149 2121.0639 -23.1 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
TIFPIGLGMAATWDMEAVAK
3434 506.2693 1515.7861 1515.8198 -22.2 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
ALELNGALKHFFR + Deamidated (NQ)
3553 857.9584 1713.9022 1713.8421 35.1 0 1 0.88 +1Score > 22 indicates identity
Score > 13 indicates homology
EGQEVEVLVLDVDNR + Deamidated (NQ)
4107 800.7671 2399.2795 2399.2366 17.9 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TPMGGLQGDLKSLTAPQLGSAAIR + Oxidation (M); 2 Deamidated (NQ)
4233 627.7908 2507.1341 2507.2503 -46.4 1 1 0.88 +1Score > 21 indicates identity
Score > 13 indicates homology
VVVQAEGDQRSQVEDLLNIHVR + 4 Deamidated (NQ)
4313 887.4829 2659.4269 2659.4579 -11.7 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MTLEGKTALVTGSTSGIGLGIAQVLAR + Oxidation (M)
2495 743.3668 742.3595 742.3609 -1.90 0 1 2 +1Score > 17 indicates identity NELPNR + Deamidated (NQ)
3005 377.5479 1129.6219 1129.5662 49.3 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
RIHMSIDSR + Oxidation (M)
3654 458.2341 1828.9073 1828.8335 40.3 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
TGDLGVMSNGELFIMGR + 2 Oxidation (M); Deamidated (NQ)
4326 893.7541 2678.2405 2678.3560 -43.2 1 1 0.83 +1Score > 22 indicates identity
Score > 13 indicates homology
MAISVFDLFKIGIGPSSSHTVGPMR + 2 Oxidation (M)
4672 752.9957 3759.9421 3760.0824 -37.3 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
DIAADFWAIAGALLISCLVTLVFVGVLMQKLIQR + Oxidation (M)
4469 851.4780 3401.8829 3401.7330 44.1 1 1 2.5 +1Score > 18 indicates identity LWKLPVVDSNAVPEGEFLVGAMNIAAQVFDR + Oxidation (M); Deamidated (NQ)
3502 414.7270 1654.8789 1654.8600 11.4 0 1 0.8 +1Score > 23 indicates identity
Score > 13 indicates homology
AGDVIPQVMQVVLER + 2 Deamidated (NQ)
3759 513.5247 2050.0697 2050.0847 -7.33 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
QAGTTVLWIEHDLEAVLR
3416 750.4149 1498.8152 1498.7926 15.1 1 1 0.92 +1Score > 22 indicates identity
Score > 13 indicates homology
MPLTKPNQELRR + Oxidation (M); Deamidated (NQ)
3314 468.8942 1403.6608 1403.7231 -44.4 0 1 0.87 +1Score > 22 indicates identity
Score > 13 indicates homology
LAGMHLFSLSNAK + Oxidation (M)
3692 932.4870 1862.9594 1863.0213 -33.2 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ANLNIRLELEHTEAIK
2577 412.6893 823.3640 823.3745 -12.7 0 1 2.3 +1Score > 17 indicates identity SQMNSIK + Oxidation (M); Deamidated (NQ)
3400 495.9464 1484.8174 1484.7697 32.1 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LAMVNPIGLEDWK
3895 750.3676 2248.0810 2248.1335 -23.4 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
HQIVALPGSDADEAQLVDTLR + Deamidated (NQ)
4258 638.3296 2549.2893 2549.2544 13.7 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MKVAINALHNAQGTPTGAGAPGTTPR + Oxidation (M); 3 Deamidated (NQ)
3463 784.4177 1566.8208 1566.8552 -21.9 1 1 0.97 +1Score > 23 indicates identity
Score > 14 indicates homology
MSTLRTLSNLLYR
4028 585.8193 2339.2481 2339.2559 -3.32 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LPEEATQIYLGMPAPLAVAALR + Oxidation (M)
3103 307.8993 1227.5681 1227.6248 -46.2 0 1 0.94 +1Score > 20 indicates identity
Score > 13 indicates homology
YSLAIYQSGAR
3159 630.8484 1259.6822 1259.6371 35.9 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
SRFVPDQQAGR
4399 1000.5029 2998.4869 2998.4899 -1.00 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LFSQMHVFLKPGELLTGEVNGQFYSR + 2 Deamidated (NQ)
4461 1131.5920 3391.7542 3391.8108 -16.7 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
KAGPALAAGCTMVLKPASQTPYSALALVELAHR
2431 344.6407 687.2668 687.2823 -22.6 0 1 0.78 +1Score > 13 indicates identity NNPGQR + 3 Deamidated (NQ)
3694 623.6233 1867.8481 1867.9176 -37.2 1 1 0.87 +1Score > 21 indicates identity
Score > 13 indicates homology
DAQEAGEVIHDPLYRR
3500 823.4119 1644.8092 1644.7606 29.6 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
DYGFAPEQLMGVFR + Oxidation (M)
4443 676.1566 3375.7466 3375.8298 -24.6 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LLGDQGLQIVSRLMGLFVCALAAQIIFTGIR + 3 Deamidated (NQ)
3476 793.3646 1584.7146 1584.7056 5.71 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
QGIADPDQFGPNAPR + 3 Deamidated (NQ)
4657 741.7944 3703.9356 3703.7729 43.9 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MAAYYNEIDPYAAQWLRNLIAAGHIAPGDVDER + Deamidated (NQ)
4041 783.4131 2347.2175 2347.1035 48.6 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ADIIIADDIEIPNNSATQMMR + Oxidation (M); Deamidated (NQ)
4196 618.5662 2470.2357 2470.2009 14.1 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LMGGDITLENQREVGGEPVADLR + 2 Deamidated (NQ)
4512 572.6227 3429.6925 3429.7142 -6.31 1 1 0.98 +1Score > 22 indicates identity
Score > 13 indicates homology
GVDAVYFPFHKANQFLGMTGLPTFLAVDVMK + Oxidation (M); Deamidated (NQ)
2739 972.4214 971.4141 971.4137 0.41 0 1 2.1 +1Score > 17 indicates identity FDNWFSR + Deamidated (NQ)
3187 425.8693 1274.5861 1274.5680 14.2 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LADEWGWDQR
4006 581.0510 2320.1749 2320.1620 5.54 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
EQGVGELMLIGGAQLYGQALEK + Oxidation (M); Deamidated (NQ)
4677 944.7374 3774.9205 3774.7433 46.9 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NDSFYQDYLNGTLDLQAYLAFSMEILAATPMAR + 2 Oxidation (M); Deamidated (NQ)
3867 742.0436 2223.1090 2223.0801 13.0 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VASVMDNSRQLTDSSVELTR + Oxidation (M)
3457 519.6210 1555.8412 1555.8504 -5.94 1 1 0.92 +1Score > 22 indicates identity
Score > 13 indicates homology
RTQLMVKPQAIDR + Deamidated (NQ)
4035 781.3995 2341.1767 2341.2317 -23.5 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
INTFLQAWLQAHNLSLQTLK + 3 Deamidated (NQ)
4374 941.5202 2821.5388 2821.4644 26.4 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
DHPGVLAQVASILSERGINIESIMQK + Oxidation (M); Deamidated (NQ)
2272 616.2888 615.2815 615.3089 -44.4 0 1 1.4 +1Score > 15 indicates identity QQQGR
2294 622.3050 621.2977 621.3235 -41.4 0 1 0.96 +1Score > 20 indicates identity
Score > 13 indicates homology
QGVYR
3967 1145.0990 2288.1834 2288.2012 -7.75 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
IIAERQVQDAFEALVTLTNR + 2 Deamidated (NQ)
3833 549.5385 2194.1249 2194.0762 22.2 1 1 0.87 +1Score > 22 indicates identity
Score > 13 indicates homology
MLALRQGMSGEIGFELQGPK + 2 Oxidation (M); Deamidated (NQ)
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