MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4024 468.6565 2338.2461 2338.1336 48.1 0 4 0.61 +1Score > 22 indicates identity
Score > 15 indicates homology
QVIEGLQQGTQDVVGAMHAGQR + Oxidation (M); Deamidated (NQ)
3869 742.3793 2224.1161 2224.0906 11.5 1 4 0.81 +1Score > 22 indicates identity
Score > 16 indicates homology
KMHGINSVPGGVNNNLSIAER + Oxidation (M); 2 Deamidated (NQ)
4652 739.1933 3690.9301 3690.7869 38.8 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TFSPNLDQMTPEQLRALAAQALQLQSQVEAMSR + Oxidation (M); 3 Deamidated (NQ)
3587 582.9578 1745.8516 1745.8618 -5.84 0 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
EQGSATPLSGLAAQLMR + Oxidation (M); Deamidated (NQ)
3050 592.3094 1182.6042 1182.6292 -21.1 1 4 0.61 +1Score > 22 indicates identity
Score > 14 indicates homology
QLIDMHVRR + Oxidation (M)
4094 797.0883 2388.2431 2388.2285 6.12 1 4 0.64 +1Score > 22 indicates identity
Score > 15 indicates homology
LDQSRFDALAPLLELSSSAAQR + Deamidated (NQ)
2812 505.6881 1009.3616 1009.3957 -33.8 0 4 0.4 +1Score > 13 indicates identity MVDAGECGR + Oxidation (M)
3740 1001.5528 2001.0910 2001.0526 19.2 0 4 0.44 +1Score > 22 indicates identity
Score > 13 indicates homology
LIVCITEGIPTLDMLDAK
3808 718.3600 2152.0582 2152.0834 -11.7 0 4 0.88 +1Score > 23 indicates identity
Score > 16 indicates homology
IYASTTNALINTGDGIGMALR + Deamidated (NQ)
4144 813.4117 2437.2133 2437.1559 23.5 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
HMGDKDLLLTWFGQFMTEPR + Oxidation (M)
2735 324.1525 969.4357 969.4556 -20.6 0 4 1.7 +1Score > 19 indicates identity FNDVFTAR + Deamidated (NQ)
2296 622.3326 621.3253 621.3459 -33.1 0 4 1.9 +1Score > 19 indicates identity HRPGR
3548 428.7166 1710.8373 1710.8281 5.40 0 4 0.76 +1Score > 22 indicates identity
Score > 15 indicates homology
TVIETTSAASVCAMVR + Oxidation (M)
2949 544.8219 1087.6292 1087.6125 15.4 0 4 2 +1Score > 19 indicates identity LSDVIVESVK
4457 679.3543 3391.7351 3391.5826 45.0 1 4 0.89 +1Score > 21 indicates identity
Score > 16 indicates homology
MPADHPRPGIVHNDYRFDNVILDADNPMR + Oxidation (M); Deamidated (NQ)
4410 1081.2500 3240.7282 3240.7176 3.27 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
RQAKPEHKPSLEELLEMLVEQALAVQPR + 2 Deamidated (NQ)
2716 478.2831 954.5516 954.5181 35.1 1 4 1.7 +1Score > 19 indicates identity HLRMLNR + Oxidation (M)
4022 584.7917 2335.1377 2335.0716 28.3 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GHQEIQADGNRYLVLENGYR + 4 Deamidated (NQ)
3269 681.3521 1360.6896 1360.7285 -28.6 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
MPEGTRIAALFR
4432 1116.5646 3346.6720 3346.6391 9.82 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
FDGYTRVLPQQSKPGEDDVLPEMVQGEALK + Deamidated (NQ)
4575 704.9774 3519.8506 3519.7308 34.1 0 4 0.94 +1Score > 20 indicates identity
Score > 16 indicates homology
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
3876 744.4206 2230.2400 2230.2182 9.77 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TNLTHPSSLRSAPQAPATLLR
3520 558.9734 1673.8984 1673.8220 45.6 1 4 0.65 +1Score > 22 indicates identity
Score > 14 indicates homology
VLAVSTEDEDVADRR
3696 625.6760 1874.0062 1874.0158 -5.12 0 4 0.86 +1Score > 22 indicates identity
Score > 16 indicates homology
AAMLEALVTALMVWISR
3008 1131.5883 1130.5810 1130.5819 -0.77 0 4 0.98 +1Score > 23 indicates identity
Score > 16 indicates homology
DESGQIIGLAK + Deamidated (NQ)
4573 587.6478 3519.8431 3519.7308 31.9 0 4 0.58 +1Score > 20 indicates identity
Score > 14 indicates homology
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
4698 635.1823 3805.0501 3804.9257 32.7 1 4 1.6 +1Score > 18 indicates identity YGLSPMAPQHAIAGIRQAVEQDEISLVVADVDWAR
3230 436.5570 1306.6492 1306.6517 -1.95 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
QLASIDQFTAGR + Deamidated (NQ)
3546 569.9800 1706.9182 1706.9203 -1.23 0 4 0.61 +1Score > 21 indicates identity
Score > 14 indicates homology
APQVELNLIPTAVQGR + 2 Deamidated (NQ)
3180 318.4148 1269.6301 1269.6466 -13.0 1 4 0.62 +1Score > 22 indicates identity
Score > 14 indicates homology
GQRGIWQPAAGK + 2 Deamidated (NQ)
2407 336.6880 671.3614 671.3854 -35.6 0 4 0.84 +1Score > 20 indicates identity
Score > 15 indicates homology
LLPDSK
3593 584.9841 1751.9305 1751.9530 -12.8 1 4 0.84 +1Score > 22 indicates identity
Score > 15 indicates homology
QSKPPGLVSALDRLNR + 2 Deamidated (NQ)
2766 984.5132 983.5059 983.5036 2.38 0 4 0.94 +1Score > 20 indicates identity
Score > 16 indicates homology
QLDQPNIR + Deamidated (NQ)
4462 848.9464 3391.7565 3391.6355 35.7 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NACTVDAVITVVDSPAVAAGTFAAYPDQVDAQR
3302 463.6033 1387.7881 1387.7361 37.5 1 4 2 +1Score > 19 indicates identity VRFGDNLPPAFR
3423 503.5894 1507.7464 1507.7518 -3.61 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
KSLFDSIQGLDER + Deamidated (NQ)
3032 575.8207 1149.6268 1149.6255 1.20 0 4 0.99 +1Score > 21 indicates identity
Score > 16 indicates homology
VSGLTPGQVHR
3653 610.6429 1828.9069 1828.8335 40.1 0 4 0.58 +1Score > 23 indicates identity
Score > 14 indicates homology
TGDLGVMSNGELFIMGR + 2 Oxidation (M); Deamidated (NQ)
3572 867.4561 1732.8976 1732.9029 -3.02 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
AGKTIMLQNIAANITR + Oxidation (M); 3 Deamidated (NQ)
4054 786.7187 2357.1343 2357.0667 28.7 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LDEQEQADFLQMYLQAMQR + Deamidated (NQ)
3667 462.4754 1845.8725 1845.9155 -23.3 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
GVVYASQAAAAHMIERR + Oxidation (M); Deamidated (NQ)
3237 658.8724 1315.7302 1315.6731 43.4 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
QAKASENLGELR + Deamidated (NQ)
2144 516.3149 515.3076 515.3067 1.76 0 3 1 +1Score > 25 indicates identity
Score > 16 indicates homology
IAANK
2764 492.7189 983.4232 983.4018 21.8 0 3 1.3 +1Score > 17 indicates identity QSPFMNSR + Oxidation (M); 2 Deamidated (NQ)
2967 549.2389 1096.4632 1096.5005 -34.0 1 3 1.2 +1Score > 17 indicates identity DAKTASAMMR + Oxidation (M)
4193 494.8551 2469.2391 2469.1660 29.6 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ETQYQYDPEGRVTQVTDALEK
3079 303.3966 1209.5573 1209.5747 -14.4 0 3 0.72 +1Score > 21 indicates identity
Score > 14 indicates homology
MPGMHQQPIR + Oxidation (M)
3185 319.6532 1274.5837 1274.6037 -15.7 1 3 0.84 +1Score > 21 indicates identity
Score > 15 indicates homology
KGSPQVACESGR
3621 597.6440 1789.9102 1789.9501 -22.3 1 3 0.72 +1Score > 23 indicates identity
Score > 14 indicates homology
NKILFENLTPLFPNK + 3 Deamidated (NQ)
3606 588.6545 1762.9417 1762.8824 33.6 1 3 0.53 +1Score > 21 indicates identity
Score > 13 indicates homology
LMAYQSQPRLDVWR + Deamidated (NQ)
2422 341.1621 680.3096 680.3316 -32.2 0 3 1.7 +1Score > 18 indicates identity MDLFR
2789 998.5562 997.5489 997.5444 4.55 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
LENLPVGQK + Deamidated (NQ)
3340 713.8707 1425.7268 1425.6996 19.1 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
KLLNELYDMMR + Deamidated (NQ)
3912 755.3561 2263.0465 2263.1015 -24.3 1 3 0.75 +1Score > 22 indicates identity
Score > 15 indicates homology
LNFIAACNAQVSQGRLSNAAR + 3 Deamidated (NQ)
3891 1123.0831 2244.1516 2244.2188 -29.9 0 3 0.62 +1Score > 22 indicates identity
Score > 14 indicates homology
LVLFGPESSLGNALMVELLSR
2867 520.7144 1039.4142 1039.4141 0.10 1 3 0.54 +1Score > 13 indicates identity FNDGRCDR + Deamidated (NQ)
3506 553.9848 1658.9326 1658.9104 13.4 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
LPQAQGVALGVAPPQGR + Deamidated (NQ)
3793 1059.5261 2117.0376 2117.1368 -46.8 1 3 0.89 +1Score > 23 indicates identity
Score > 15 indicates homology
KYLTLDGGLAIDNVLNELR + Deamidated (NQ)
3095 613.2867 1224.5588 1224.6033 -36.3 1 3 0.52 +1Score > 20 indicates identity
Score > 13 indicates homology
MQGSSLFARGR + Oxidation (M)
4361 690.8643 2759.4281 2759.3833 16.2 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
IQAQAVASQIMPLGNITQMTTEERK + 2 Deamidated (NQ)
4026 1170.5691 2339.1236 2339.1613 -16.1 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
DHTVLLIEHNMSIVMSISQR + Oxidation (M); Deamidated (NQ)
4468 1133.5643 3397.6711 3397.6033 20.0 0 3 0.79 +1Score > 22 indicates identity
Score > 15 indicates homology
LDASGVALEEAHVPGVPFPMPTTGAEVMWNMK + Oxidation (M); Deamidated (NQ)
3161 421.2050 1260.5932 1260.5955 -1.81 1 3 0.66 +1Score > 22 indicates identity
Score > 14 indicates homology
IPDMRMDDIR
3807 538.2701 2149.0513 2149.0395 5.49 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LMLQQMIDASQVNVNGVVR + 2 Oxidation (M); 3 Deamidated (NQ)
3019 569.2905 1136.5664 1136.6012 -30.6 1 3 0.9 +1Score > 21 indicates identity
Score > 15 indicates homology
MNQKLTVFR + Deamidated (NQ)
4303 665.3273 2657.2801 2657.3676 -32.9 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
FELGMVRFNLAFIDGTLAFPVQR + Oxidation (M); Deamidated (NQ)
4527 862.1707 3444.6537 3444.5436 32.0 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MLNFLENASDLAEDGSEIDVEMLQSLLGDSR + 2 Oxidation (M); 2 Deamidated (NQ)
3149 419.5241 1255.5505 1255.6091 -46.7 1 3 2.2 +1Score > 19 indicates identity ALQQHREAMR + Oxidation (M); Deamidated (NQ)
4261 854.4138 2560.2196 2560.2499 -11.8 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
SVDSLEWETLKWVTWFNHQR
2579 412.7535 823.4924 823.4776 18.0 1 3 1.1 +1Score > 16 indicates identity AARLTHR
3695 938.0098 1874.0050 1874.0158 -5.72 0 3 0.76 +1Score > 22 indicates identity
Score > 14 indicates homology
AAMLEALVTALMVWISR
4201 620.0613 2476.2161 2476.1904 10.4 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
SDMLAAVSAHQLAYEIASGELQR + Oxidation (M); Deamidated (NQ)
2917 536.2888 1070.5630 1070.5971 -31.8 0 3 0.97 +1Score > 22 indicates identity
Score > 15 indicates homology
ALQQLLEQK + Deamidated (NQ)
2659 454.2608 906.5070 906.4923 16.3 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TLGKEFGR
3182 424.2177 1269.6313 1269.6830 -40.7 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LESWRQGPLGK
3217 649.7749 1297.5352 1297.5939 -45.2 0 3 1.1 +1Score > 16 indicates identity DFALNSGYPASR + Deamidated (NQ)
3701 470.4797 1877.8897 1877.8392 26.9 1 3 0.77 +1Score > 22 indicates identity
Score > 14 indicates homology
KGPQTGEGDEHTPGGPGQK + 2 Deamidated (NQ)
3564 575.9845 1724.9317 1724.8482 48.4 1 3 0.87 +1Score > 22 indicates identity
Score > 15 indicates homology
HAPAQPLYTEQDAKR + Deamidated (NQ)
3806 1075.5314 2149.0482 2149.0871 -18.1 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
RTGATMMQELGVTLEIIDR + Oxidation (M)
3505 552.6356 1654.8850 1654.9116 -16.1 0 3 0.56 +1Score > 22 indicates identity
Score > 13 indicates homology
VFALIAPLAPMLDQR + Deamidated (NQ)
3137 624.8171 1247.6196 1247.6735 -43.1 1 3 0.77 +1Score > 23 indicates identity
Score > 14 indicates homology
LAGDHDLPVRR
3914 1133.0337 2264.0528 2264.1436 -40.1 0 3 0.59 +1Score > 22 indicates identity
Score > 13 indicates homology
IEGNAPWLQLAQLPASEAAQR + 2 Deamidated (NQ)
3933 570.0306 2276.0933 2276.1695 -33.5 1 3 0.54 +1Score > 22 indicates identity
Score > 13 indicates homology
MNTRIDPSQHLLGQGPLINR + Oxidation (M); Deamidated (NQ)
3689 466.4839 1861.9065 1861.8516 29.5 0 3 0.76 +1Score > 22 indicates identity
Score > 14 indicates homology
NNEHVDMTNLLLYQR + 3 Deamidated (NQ)
4083 793.0912 2376.2518 2376.1553 40.6 0 3 0.63 +1Score > 21 indicates identity
Score > 13 indicates homology
ATGGMLITPIGLDDVSQAEDMVK + Oxidation (M)
2156 528.2806 527.2733 527.2816 -15.7 0 3 0.56 +1Score > 13 indicates identity QAGPR
3934 759.7530 2276.2372 2276.1511 37.8 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
LFTFHADICTLPDTEKQIK
3913 566.7689 2263.0465 2263.1042 -25.5 0 3 0.8 +1Score > 22 indicates identity
Score > 14 indicates homology
LVEAGLVTSMTDWLQLDAER + Oxidation (M); Deamidated (NQ)
3879 447.4275 2232.1011 2232.0343 29.9 0 3 0.95 +1Score > 23 indicates identity
Score > 15 indicates homology
GYSPNQMYAQAMVYEPLVR + Oxidation (M)
3145 627.8398 1253.6650 1253.6663 -0.97 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
RMGHAGAIISGGK
4464 679.3632 3391.7796 3391.6905 26.3 1 3 0.78 +1Score > 20 indicates identity
Score > 14 indicates homology
MAVSLQAPVVQYSFRLDDTQVPVNPMIGQR + 2 Oxidation (M); Deamidated (NQ)
3279 685.8653 1369.7160 1369.6547 44.8 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
SADQYAKMGLAAK + Oxidation (M); Deamidated (NQ)
4034 586.3013 2341.1761 2341.2199 -18.7 0 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
DELLTLAAGSPLMAVSLQAQGVR + 2 Deamidated (NQ)
4549 694.5335 3467.6311 3467.4666 47.5 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SSNFVVAQCEMTPQGLPAMHMTSFQASYDSK + Oxidation (M); 3 Deamidated (NQ)
3948 457.4527 2282.2271 2282.1576 30.4 1 3 0.67 +1Score > 21 indicates identity
Score > 14 indicates homology
SGLTTSQVMERVVYAAIIDGR + Oxidation (M); Deamidated (NQ)
4053 786.7120 2357.1142 2357.1863 -30.6 0 3 0.6 +1Score > 23 indicates identity
Score > 13 indicates homology
NGATAQLLYVAPGAEDQLEGALR + Deamidated (NQ)
3932 570.0255 2276.0729 2276.1113 -16.9 0 3 0.55 +1Score > 22 indicates identity
Score > 13 indicates homology
ALFQQPFNDLLFQAQTVHR + 4 Deamidated (NQ)
2989 373.2181 1116.6325 1116.5961 32.6 0 3 0.89 +1Score > 21 indicates identity
Score > 15 indicates homology
LGMALQAQLR + Oxidation (M); Deamidated (NQ)
3047 588.3392 1174.6638 1174.6281 30.4 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QGFVRLPAMR + Deamidated (NQ)
3186 638.2992 1274.5838 1274.6215 -29.5 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
TQGIDRDSLNR + Deamidated (NQ)
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