| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 324.1612 | 646.3078 | 646.3187 | -16.8 | 0 | 6 | 0.95 | 1Score > 22 indicates identityScore > 18 indicates homology |
NFPNR | |
| 856.9595 | 1711.9044 | 1711.8311 | 42.8 | 1 | 6 | 0.47 | 1Score > 22 indicates identityScore > 15 indicates homology |
ETLRDAEHVQDMIR | |
| 432.7094 | 1726.8085 | 1726.8890 | -46.6 | 1 | 6 | 0.58 | 1Score > 21 indicates identityScore > 16 indicates homology |
SNPFPLPAQKNVVSAR + 3 Deamidated (NQ) | |
| 331.6669 | 661.3192 | 661.3143 | 7.44 | 1 | 6 | 0.92 | 1Score > 23 indicates identityScore > 18 indicates homology |
SQDRR + Deamidated (NQ) | |
| 422.8442 | 1265.5108 | 1265.5499 | -30.9 | 0 | 6 | 0.42 | 1Score > 15 indicates identity |
AWDDVSWMTR | |
| 683.3257 | 682.3184 | 682.3147 | 5.51 | 0 | 6 | 0.9 | 1Score > 18 indicates identity |
HNQQR + Deamidated (NQ) | |
| 506.7181 | 1011.4216 | 1011.4661 | -44.0 | 0 | 6 | 0.71 | 1Score > 17 indicates identity |
YPVYDAER | |
| 618.6835 | 1853.0287 | 1852.9604 | 36.8 | 1 | 6 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
LMYDKSIESLEILQR + Oxidation (M) | |
| 686.3236 | 685.3163 | 685.3395 | -33.8 | 0 | 6 | 0.79 | 1Score > 17 indicates identity |
LDPADR | |
| 1145.5602 | 3433.6588 | 3433.7850 | -36.8 | 1 | 6 | 0.47 | 1Score > 22 indicates identityScore > 15 indicates homology |
DVAFGVTVAGRPVSMPQMQRTVGLFINSVALR + Oxidation (M); 2 Deamidated (NQ) | |
| 537.3239 | 1072.6332 | 1072.5989 | 32.0 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
RTLGGNISVR + Deamidated (NQ) | |
| 704.9839 | 3519.8831 | 3519.7308 | 43.3 | 0 | 6 | 1.3 | 1Score > 19 indicates identity |
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ) | |
| 362.8491 | 1085.5255 | 1085.5465 | -19.4 | 0 | 6 | 1 | 1Score > 21 indicates identityScore > 18 indicates homology |
LDNLAEQQR | |
| 444.9174 | 1331.7304 | 1331.7449 | -10.9 | 1 | 6 | 1 | 1Score > 21 indicates identityScore > 18 indicates homology |
LQLNVKNLFDK + Deamidated (NQ) | |
| 486.7139 | 971.4132 | 971.3654 | 49.2 | 0 | 6 | 0.74 | 1Score > 17 indicates identity |
MNENQYR + Oxidation (M); 2 Deamidated (NQ) | |
| 523.9394 | 1568.7964 | 1568.7888 | 4.82 | 1 | 6 | 0.43 | 1Score > 22 indicates identityScore > 14 indicates homology |
NLFLWSGRSWFR + Deamidated (NQ) | |
| 930.9330 | 1859.8514 | 1859.9013 | -26.8 | 0 | 6 | 0.97 | 1Score > 21 indicates identityScore > 18 indicates homology |
NDQSWLLSQTASLPSGR + Deamidated (NQ) | |
| 427.2455 | 852.4764 | 852.4341 | 49.6 | 0 | 6 | 0.98 | 1Score > 18 indicates identity |
SLYDTVR | |
| 519.7440 | 1037.4734 | 1037.4414 | 30.9 | 0 | 6 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
FAAGEANSDR + Deamidated (NQ) | |
| 621.2925 | 1860.8557 | 1860.8853 | -15.9 | 0 | 6 | 0.69 | 1Score > 21 indicates identityScore > 16 indicates homology |
NDQSWLLSQTASLPSGR + 2 Deamidated (NQ) | |
| 541.2552 | 1080.4958 | 1080.5451 | -45.6 | 1 | 5 | 0.93 | 1Score > 20 indicates identityScore > 18 indicates homology |
SDINNKYVK + Deamidated (NQ) | |
| 414.7278 | 1654.8821 | 1654.8204 | 37.3 | 1 | 5 | 0.33 | 1Score > 22 indicates identityScore > 13 indicates homology |
LVRMMTTSMGALANK + 2 Oxidation (M) | |
| 780.7142 | 2339.1208 | 2339.1613 | -17.3 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
DHTVLLIEHNMSIVMSISQR + Oxidation (M); Deamidated (NQ) | |
| 534.8253 | 1067.6360 | 1067.6339 | 2.04 | 0 | 5 | 1 | 1Score > 18 indicates identity |
LVPAALEISR | |
| 492.9374 | 1475.7904 | 1475.7409 | 33.6 | 0 | 5 | 0.33 | 1Score > 22 indicates identityScore > 13 indicates homology |
QLFLDAFEHSLR + Deamidated (NQ) | |
| 634.0161 | 1899.0265 | 1899.0465 | -10.6 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
LFVANLEVLDALEGGLAR | |
| 572.3663 | 571.3590 | 571.3442 | 26.0 | 0 | 5 | 0.97 | 1Score > 22 indicates identityScore > 18 indicates homology |
LAAAAR | |
| 558.7762 | 1115.5378 | 1115.5822 | -39.8 | 0 | 5 | 0.35 | 1Score > 22 indicates identityScore > 13 indicates homology |
TTLEIPASQR + Deamidated (NQ) | |
| 388.1734 | 1161.4984 | 1161.5513 | -45.6 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
DNTLQSVIDR + 2 Deamidated (NQ) | |
| 477.2265 | 952.4384 | 952.4727 | -35.9 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
TRTTDFGR | |
| 562.8401 | 1123.6656 | 1123.6349 | 27.3 | 0 | 5 | 0.7 | 1Score > 16 indicates identity |
VPEAAAALGLGR | |
| 575.3158 | 1148.6170 | 1148.5682 | 42.5 | 0 | 5 | 0.99 | 1Score > 22 indicates identityScore > 18 indicates homology |
TMVILDGGMGR | |
| 669.8290 | 1337.6434 | 1337.6762 | -24.5 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
LGGNKSMGVFATR + Deamidated (NQ) | |
| 632.9736 | 1895.8990 | 1895.9813 | -43.4 | 1 | 5 | 0.32 | 1Score > 22 indicates identityScore > 13 indicates homology |
NLSHAAERLGVSQSALSR + Deamidated (NQ) | |
| 649.8420 | 1297.6694 | 1297.6738 | -3.38 | 1 | 5 | 0.88 | 1Score > 22 indicates identityScore > 17 indicates homology |
LLNEDQPRSAR | |
| 367.1860 | 732.3574 | 732.3766 | -26.2 | 0 | 5 | 0.53 | 1Score > 22 indicates identityScore > 15 indicates homology |
VVSASNR + Deamidated (NQ) | |
| 756.8638 | 1511.7130 | 1511.7806 | -44.7 | 1 | 5 | 0.37 | 1Score > 21 indicates identityScore > 13 indicates homology |
MGAWLSNVSLKYK + Oxidation (M) | |
| 385.6938 | 1538.7461 | 1538.7446 | 0.98 | 1 | 5 | 0.95 | 1Score > 22 indicates identityScore > 17 indicates homology |
MQLGASCRLQYGR | |
| 396.4533 | 1581.7841 | 1581.7899 | -3.68 | 1 | 5 | 0.7 | 1Score > 22 indicates identityScore > 16 indicates homology |
RDALYSQLQAFNR + Deamidated (NQ) | |
| 735.3988 | 1468.7830 | 1468.7960 | -8.79 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
LMAGDLLPVGVPSGK + Oxidation (M) | |
| 516.3152 | 515.3079 | 515.3319 | -46.5 | 0 | 5 | 1 | 1Score > 25 indicates identityScore > 18 indicates homology |
LLLSA | |
| 824.4216 | 2470.2430 | 2470.1533 | 36.3 | 0 | 5 | 0.52 | 1Score > 22 indicates identityScore > 15 indicates homology |
SNGAYATASNQAMLTAALEGLIER + Oxidation (M); 3 Deamidated (NQ) | |
| 680.0853 | 2716.3121 | 2716.3153 | -1.19 | 0 | 5 | 0.41 | 1Score > 22 indicates identityScore > 14 indicates homology |
GYSLTVPITNSDMAPTSTILDETYK | |
| 324.1669 | 646.3192 | 646.3108 | 13.0 | 0 | 5 | 0.59 | 1Score > 23 indicates identityScore > 15 indicates homology |
MNLPR + Oxidation (M); Deamidated (NQ) | |
| 620.5694 | 2478.2485 | 2478.2040 | 17.9 | 1 | 5 | 0.6 | 1Score > 23 indicates identityScore > 15 indicates homology |
VTLDEWAHDHFRTPPSSNTLR | |
| 437.7251 | 1746.8713 | 1746.8862 | -8.53 | 1 | 5 | 0.9 | 1Score > 23 indicates identityScore > 17 indicates homology |
STTFYELMLEKLTR + Oxidation (M) | |
| 704.9792 | 3519.8596 | 3519.7308 | 36.6 | 0 | 5 | 0.94 | 1Score > 20 indicates identityScore > 17 indicates homology |
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ) | |
| 490.2636 | 489.2563 | 489.2547 | 3.35 | 0 | 5 | 1 | 1Score > 25 indicates identityScore > 17 indicates homology |
SNAAK | |
| 862.1810 | 3444.6949 | 3444.7558 | -17.7 | 1 | 5 | 0.54 | 1Score > 22 indicates identityScore > 15 indicates homology |
NTMKGLLDSAAIEAAGVDGSLRPEQLDLAAFVR + Oxidation (M); Deamidated (NQ) | |
| 474.9821 | 1895.8993 | 1895.9411 | -22.0 | 1 | 5 | 0.61 | 1Score > 22 indicates identityScore > 15 indicates homology |
LQASSGAAMTAELGRAYAK + Deamidated (NQ) | |
| 620.8685 | 1239.7224 | 1239.7386 | -13.0 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
LLRPWLRMR | |
| 841.4677 | 840.4604 | 840.4606 | -0.21 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
APQALWR | |
| 314.1614 | 939.4624 | 939.4484 | 14.9 | 0 | 5 | 0.86 | 1Score > 20 indicates identityScore > 17 indicates homology |
AYNTALMR + Deamidated (NQ) | |
| 590.2917 | 2357.1377 | 2357.0667 | 30.1 | 0 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
LDEQEQADFLQMYLQAMQR + Deamidated (NQ) | |
| 627.8400 | 1253.6654 | 1253.6867 | -16.9 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
LLLNSIGPNSPK + 2 Deamidated (NQ) | |
| 742.7231 | 2225.1475 | 2225.0919 | 25.0 | 1 | 5 | 0.56 | 1Score > 22 indicates identityScore > 15 indicates homology |
MALTVNTNITSMSVQKNLNK + Oxidation (M); 3 Deamidated (NQ) | |
| 410.2197 | 1227.6373 | 1227.6459 | -7.01 | 0 | 5 | 0.84 | 1Score > 23 indicates identityScore > 17 indicates homology |
QDLAQLIAEAR + Deamidated (NQ) | |
| 680.8889 | 1359.7632 | 1359.7874 | -17.8 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
LLGGPQAGIIVGHK + Deamidated (NQ) | |
| 778.4140 | 2332.2202 | 2332.1157 | 44.8 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
GNDFRLLGCIPYQPELNAPR + 3 Deamidated (NQ) | |
| 626.3044 | 2501.1885 | 2501.2298 | -16.5 | 1 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
QYIANLSWQATHDALTGLANRR + 3 Deamidated (NQ) | |
| 566.6022 | 1696.7848 | 1696.8420 | -33.8 | 1 | 5 | 0.85 | 1Score > 21 indicates identityScore > 17 indicates homology |
FVDQDAQKLDYLSR | |
| 786.0477 | 2355.1213 | 2355.1563 | -14.9 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
DHTVLLIEHNMSIVMSISQR + 2 Oxidation (M); Deamidated (NQ) | |
| 318.1681 | 634.3216 | 634.3286 | -11.0 | 1 | 5 | 0.88 | 1Score > 22 indicates identityScore > 17 indicates homology |
DKTSGK | |
| 446.8885 | 1337.6437 | 1337.6728 | -21.8 | 1 | 5 | 0.48 | 1Score > 22 indicates identityScore > 14 indicates homology |
SRWYETQLVR + Deamidated (NQ) | |
| 354.1776 | 706.3406 | 706.3650 | -34.4 | 0 | 5 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
NLGFEK | |
| 918.4555 | 1834.8964 | 1834.9676 | -38.8 | 1 | 5 | 0.47 | 1Score > 23 indicates identityScore > 14 indicates homology |
QILSNLISNAIKFTDR + 3 Deamidated (NQ) | |
| 482.7189 | 963.4232 | 963.4630 | -41.3 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
MPAVKSCR + Oxidation (M) | |
| 347.9299 | 1387.6905 | 1387.7095 | -13.7 | 0 | 5 | 0.94 | 1Score > 23 indicates identityScore > 17 indicates homology |
ELITDLNWASAR | |
| 513.9229 | 1538.7469 | 1538.7801 | -21.6 | 1 | 5 | 0.84 | 1Score > 22 indicates identityScore > 16 indicates homology |
ALGRNDELAHSSIR + Deamidated (NQ) | |
| 736.0005 | 3674.9661 | 3674.7919 | 47.4 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
TFSPNLDQMTPEQLRALAAQALQLQSQVEAMSR + 3 Deamidated (NQ) | |
| 1141.2413 | 3420.7021 | 3420.6620 | 11.7 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
RQFDEGEEAFLVTMSAQLAGVIAHAEATGSIR + Oxidation (M); Deamidated (NQ) | |
| 723.0438 | 2166.1096 | 2166.0579 | 23.8 | 1 | 5 | 0.42 | 1Score > 22 indicates identityScore > 13 indicates homology |
KVAAADYLTQQQDELTLEK + 3 Deamidated (NQ) | |
| 312.1847 | 622.3548 | 622.3438 | 17.7 | 0 | 5 | 1 | 1Score > 17 indicates identity |
LYTAR | |
| 1143.5800 | 1142.5727 | 1142.5655 | 6.33 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
TRAQSFMFR | |
| 500.2807 | 1497.8203 | 1497.8092 | 7.39 | 1 | 5 | 0.8 | 1Score > 22 indicates identityScore > 16 indicates homology |
KAWWLTEAGPALR | |
| 571.6426 | 1711.9060 | 1711.8417 | 37.5 | 0 | 5 | 0.69 | 1Score > 22 indicates identityScore > 16 indicates homology |
ELVAYFGTQEVTAER | |
| 453.6155 | 2263.0411 | 2263.1458 | -46.2 | 1 | 5 | 0.69 | 1Score > 22 indicates identityScore > 15 indicates homology |
RHHEAIGQGDALNDVVLHPGK + Deamidated (NQ) | |
| 784.7138 | 2351.1196 | 2351.1249 | -2.27 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
VPAEAMANVRNDMYLTSEAIR + Deamidated (NQ) | |
| 646.5656 | 2582.2333 | 2582.2104 | 8.85 | 1 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
QAMDMAVNKEAILQAVYQDAGQR + 2 Oxidation (M); Deamidated (NQ) | |
| 679.5521 | 3392.7241 | 3392.6195 | 30.8 | 0 | 4 | 0.65 | 1Score > 21 indicates identityScore > 15 indicates homology |
NACTVDAVITVVDSPAVAAGTFAAYPDQVDAQR + Deamidated (NQ) | |
| 635.3127 | 1268.6108 | 1268.5819 | 22.8 | 1 | 4 | 0.62 | 1Score > 21 indicates identityScore > 15 indicates homology |
EMRTGQLFDR + Oxidation (M); Deamidated (NQ) | |
| 884.7158 | 3534.8341 | 3534.8100 | 6.82 | 1 | 4 | 0.55 | 1Score > 21 indicates identityScore > 14 indicates homology |
DGRLRPGMTAQVSLRPGENADDSLLLPAEAVIR + Oxidation (M); 2 Deamidated (NQ) | |
| 738.9002 | 1475.7858 | 1475.7845 | 0.93 | 1 | 4 | 0.81 | 1Score > 22 indicates identityScore > 16 indicates homology |
LRPVQSGGGSRFSK + Deamidated (NQ) | |
| 596.9838 | 3575.8591 | 3575.7969 | 17.4 | 1 | 4 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
LGLEMTAQQIYSLLHKEYLQANAPYALVSHR + Oxidation (M); 3 Deamidated (NQ) | |
| 505.2956 | 1008.5766 | 1008.5716 | 5.00 | 0 | 4 | 1.3 | 1Score > 18 indicates identity |
LTHLADALR | |
| 901.4554 | 2701.3444 | 2701.3096 | 12.9 | 1 | 4 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
RASWQEAASSSLTELFAPQIHQSR + 2 Deamidated (NQ) | |
| 749.0820 | 2244.2242 | 2244.1970 | 12.1 | 1 | 4 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
LRVVLMPLSTQSEMVEQLR + Oxidation (M) | |
| 398.7562 | 795.4978 | 795.4967 | 1.50 | 0 | 4 | 0.37 | 1Score > 13 indicates identity |
LQPLLGR | |
| 1142.1198 | 2282.2250 | 2282.2304 | -2.36 | 1 | 4 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
LVGPTGQVVLADINDSMLKVGR + Deamidated (NQ) | |
| 704.9748 | 3519.8376 | 3519.7304 | 30.5 | 1 | 4 | 0.45 | 1Score > 20 indicates identityScore > 13 indicates homology |
NACTVDAVITVVDSPAVAAGTFAAYPDQVDAQRK | |
| 445.4695 | 1777.8489 | 1777.9151 | -37.3 | 1 | 4 | 0.42 | 1Score > 23 indicates identityScore > 13 indicates homology |
AFAKQFDLPADLTWR | |
| 442.7765 | 883.5384 | 883.5239 | 16.4 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
VGQALAGLR | |
| 633.3233 | 1264.6320 | 1264.6663 | -27.1 | 0 | 4 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
LVNTVQLTYGR + 2 Deamidated (NQ) | |
| 1001.5269 | 2001.0392 | 2001.0452 | -2.98 | 1 | 4 | 0.57 | 1Score > 23 indicates identityScore > 14 indicates homology |
LLGKDVLEQLGQTMEAQK + Deamidated (NQ) | |
| 549.2776 | 1644.8110 | 1644.8756 | -39.3 | 1 | 4 | 0.69 | 1Score > 23 indicates identityScore > 15 indicates homology |
SLDNKLASLSPEMLK | |
| 528.6230 | 1582.8472 | 1582.7740 | 46.2 | 0 | 4 | 0.58 | 1Score > 21 indicates identityScore > 14 indicates homology |
ANNFGGQGLTTFTVR + Deamidated (NQ) | |
| 544.2646 | 1086.5146 | 1086.5458 | -28.7 | 1 | 4 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
KDPFNPVNR + Deamidated (NQ) | |
| 602.9877 | 1805.9413 | 1805.9676 | -14.6 | 1 | 4 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
FEPGSFNVLLGRTLAGK + Deamidated (NQ) | |
| 469.9289 | 1406.7649 | 1406.7935 | -20.4 | 1 | 4 | 0.67 | 1Score > 20 indicates identityScore > 15 indicates homology |
LVHFRLFGPHGK | |
| 450.6247 | 2248.0871 | 2248.1964 | -48.6 | 1 | 4 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
LGLRYDIRPDLQVYGNLSR + Deamidated (NQ) |
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