MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2361 324.1612 646.3078 646.3187 -16.8 0 6 0.95 +1Score > 22 indicates identity
Score > 18 indicates homology
NFPNR
3551 856.9595 1711.9044 1711.8311 42.8 1 6 0.47 +1Score > 22 indicates identity
Score > 15 indicates homology
ETLRDAEHVQDMIR
3567 432.7094 1726.8085 1726.8890 -46.6 1 6 0.58 +1Score > 21 indicates identity
Score > 16 indicates homology
SNPFPLPAQKNVVSAR + 3 Deamidated (NQ)
2391 331.6669 661.3192 661.3143 7.44 1 6 0.92 +1Score > 23 indicates identity
Score > 18 indicates homology
SQDRR + Deamidated (NQ)
3170 422.8442 1265.5108 1265.5499 -30.9 0 6 0.42 +1Score > 15 indicates identity AWDDVSWMTR
2425 683.3257 682.3184 682.3147 5.51 0 6 0.9 +1Score > 18 indicates identity HNQQR + Deamidated (NQ)
2817 506.7181 1011.4216 1011.4661 -44.0 0 6 0.71 +1Score > 17 indicates identity YPVYDAER
3679 618.6835 1853.0287 1852.9604 36.8 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology
LMYDKSIESLEILQR + Oxidation (M)
2426 686.3236 685.3163 685.3395 -33.8 0 6 0.79 +1Score > 17 indicates identity LDPADR
4514 1145.5602 3433.6588 3433.7850 -36.8 1 6 0.47 +1Score > 22 indicates identity
Score > 15 indicates homology
DVAFGVTVAGRPVSMPQMQRTVGLFINSVALR + Oxidation (M); 2 Deamidated (NQ)
2918 537.3239 1072.6332 1072.5989 32.0 1 6 1.1 +1Score > 19 indicates identity RTLGGNISVR + Deamidated (NQ)
4581 704.9839 3519.8831 3519.7308 43.3 0 6 1.3 +1Score > 19 indicates identity AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
2944 362.8491 1085.5255 1085.5465 -19.4 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology
LDNLAEQQR
3248 444.9174 1331.7304 1331.7449 -10.9 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology
LQLNVKNLFDK + Deamidated (NQ)
2738 486.7139 971.4132 971.3654 49.2 0 6 0.74 +1Score > 17 indicates identity MNENQYR + Oxidation (M); 2 Deamidated (NQ)
3464 523.9394 1568.7964 1568.7888 4.82 1 6 0.43 +1Score > 22 indicates identity
Score > 14 indicates homology
NLFLWSGRSWFR + Deamidated (NQ)
3681 930.9330 1859.8514 1859.9013 -26.8 0 6 0.97 +1Score > 21 indicates identity
Score > 18 indicates homology
NDQSWLLSQTASLPSGR + Deamidated (NQ)
2614 427.2455 852.4764 852.4341 49.6 0 6 0.98 +1Score > 18 indicates identity SLYDTVR
2864 519.7440 1037.4734 1037.4414 30.9 0 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology
FAAGEANSDR + Deamidated (NQ)
3683 621.2925 1860.8557 1860.8853 -15.9 0 6 0.69 +1Score > 21 indicates identity
Score > 16 indicates homology
NDQSWLLSQTASLPSGR + 2 Deamidated (NQ)
2925 541.2552 1080.4958 1080.5451 -45.6 1 5 0.93 +1Score > 20 indicates identity
Score > 18 indicates homology
SDINNKYVK + Deamidated (NQ)
3503 414.7278 1654.8821 1654.8204 37.3 1 5 0.33 +1Score > 22 indicates identity
Score > 13 indicates homology
LVRMMTTSMGALANK + 2 Oxidation (M)
4025 780.7142 2339.1208 2339.1613 -17.3 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
DHTVLLIEHNMSIVMSISQR + Oxidation (M); Deamidated (NQ)
2913 534.8253 1067.6360 1067.6339 2.04 0 5 1 +1Score > 18 indicates identity LVPAALEISR
3390 492.9374 1475.7904 1475.7409 33.6 0 5 0.33 +1Score > 22 indicates identity
Score > 13 indicates homology
QLFLDAFEHSLR + Deamidated (NQ)
3714 634.0161 1899.0265 1899.0465 -10.6 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
LFVANLEVLDALEGGLAR
2216 572.3663 571.3590 571.3442 26.0 0 5 0.97 +1Score > 22 indicates identity
Score > 18 indicates homology
LAAAAR
2988 558.7762 1115.5378 1115.5822 -39.8 0 5 0.35 +1Score > 22 indicates identity
Score > 13 indicates homology
TTLEIPASQR + Deamidated (NQ)
3036 388.1734 1161.4984 1161.5513 -45.6 0 5 1.1 +1Score > 18 indicates identity DNTLQSVIDR + 2 Deamidated (NQ)
2710 477.2265 952.4384 952.4727 -35.9 1 5 1.5 +1Score > 19 indicates identity TRTTDFGR
2999 562.8401 1123.6656 1123.6349 27.3 0 5 0.7 +1Score > 16 indicates identity VPEAAAALGLGR
3027 575.3158 1148.6170 1148.5682 42.5 0 5 0.99 +1Score > 22 indicates identity
Score > 18 indicates homology
TMVILDGGMGR
3253 669.8290 1337.6434 1337.6762 -24.5 1 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
LGGNKSMGVFATR + Deamidated (NQ)
3712 632.9736 1895.8990 1895.9813 -43.4 1 5 0.32 +1Score > 22 indicates identity
Score > 13 indicates homology
NLSHAAERLGVSQSALSR + Deamidated (NQ)
3218 649.8420 1297.6694 1297.6738 -3.38 1 5 0.88 +1Score > 22 indicates identity
Score > 17 indicates homology
LLNEDQPRSAR
2492 367.1860 732.3574 732.3766 -26.2 0 5 0.53 +1Score > 22 indicates identity
Score > 15 indicates homology
VVSASNR + Deamidated (NQ)
3428 756.8638 1511.7130 1511.7806 -44.7 1 5 0.37 +1Score > 21 indicates identity
Score > 13 indicates homology
MGAWLSNVSLKYK + Oxidation (M)
3446 385.6938 1538.7461 1538.7446 0.98 1 5 0.95 +1Score > 22 indicates identity
Score > 17 indicates homology
MQLGASCRLQYGR
3471 396.4533 1581.7841 1581.7899 -3.68 1 5 0.7 +1Score > 22 indicates identity
Score > 16 indicates homology
RDALYSQLQAFNR + Deamidated (NQ)
3377 735.3988 1468.7830 1468.7960 -8.79 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
LMAGDLLPVGVPSGK + Oxidation (M)
2146 516.3152 515.3079 515.3319 -46.5 0 5 1 +1Score > 25 indicates identity
Score > 18 indicates homology
LLLSA
4197 824.4216 2470.2430 2470.1533 36.3 0 5 0.52 +1Score > 22 indicates identity
Score > 15 indicates homology
SNGAYATASNQAMLTAALEGLIER + Oxidation (M); 3 Deamidated (NQ)
4356 680.0853 2716.3121 2716.3153 -1.19 0 5 0.41 +1Score > 22 indicates identity
Score > 14 indicates homology
GYSLTVPITNSDMAPTSTILDETYK
2362 324.1669 646.3192 646.3108 13.0 0 5 0.59 +1Score > 23 indicates identity
Score > 15 indicates homology
MNLPR + Oxidation (M); Deamidated (NQ)
4204 620.5694 2478.2485 2478.2040 17.9 1 5 0.6 +1Score > 23 indicates identity
Score > 15 indicates homology
VTLDEWAHDHFRTPPSSNTLR
3590 437.7251 1746.8713 1746.8862 -8.53 1 5 0.9 +1Score > 23 indicates identity
Score > 17 indicates homology
STTFYELMLEKLTR + Oxidation (M)
4578 704.9792 3519.8596 3519.7308 36.6 0 5 0.94 +1Score > 20 indicates identity
Score > 17 indicates homology
AEPAGPSVVVAMAVAALGYGIMNLLMIQASMHMK + 3 Oxidation (M); Deamidated (NQ)
2092 490.2636 489.2563 489.2547 3.35 0 5 1 +1Score > 25 indicates identity
Score > 17 indicates homology
SNAAK
4529 862.1810 3444.6949 3444.7558 -17.7 1 5 0.54 +1Score > 22 indicates identity
Score > 15 indicates homology
NTMKGLLDSAAIEAAGVDGSLRPEQLDLAAFVR + Oxidation (M); Deamidated (NQ)
3713 474.9821 1895.8993 1895.9411 -22.0 1 5 0.61 +1Score > 22 indicates identity
Score > 15 indicates homology
LQASSGAAMTAELGRAYAK + Deamidated (NQ)
3123 620.8685 1239.7224 1239.7386 -13.0 1 5 1.2 +1Score > 18 indicates identity LLRPWLRMR
2599 841.4677 840.4604 840.4606 -0.21 0 5 1.3 +1Score > 19 indicates identity APQALWR
2690 314.1614 939.4624 939.4484 14.9 0 5 0.86 +1Score > 20 indicates identity
Score > 17 indicates homology
AYNTALMR + Deamidated (NQ)
4055 590.2917 2357.1377 2357.0667 30.1 0 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
LDEQEQADFLQMYLQAMQR + Deamidated (NQ)
3146 627.8400 1253.6654 1253.6867 -16.9 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LLLNSIGPNSPK + 2 Deamidated (NQ)
3870 742.7231 2225.1475 2225.0919 25.0 1 5 0.56 +1Score > 22 indicates identity
Score > 15 indicates homology
MALTVNTNITSMSVQKNLNK + Oxidation (M); 3 Deamidated (NQ)
3105 410.2197 1227.6373 1227.6459 -7.01 0 5 0.84 +1Score > 23 indicates identity
Score > 17 indicates homology
QDLAQLIAEAR + Deamidated (NQ)
3266 680.8889 1359.7632 1359.7874 -17.8 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LLGGPQAGIIVGHK + Deamidated (NQ)
4018 778.4140 2332.2202 2332.1157 44.8 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
GNDFRLLGCIPYQPELNAPR + 3 Deamidated (NQ)
4231 626.3044 2501.1885 2501.2298 -16.5 1 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
QYIANLSWQATHDALTGLANRR + 3 Deamidated (NQ)
3530 566.6022 1696.7848 1696.8420 -33.8 1 5 0.85 +1Score > 21 indicates identity
Score > 17 indicates homology
FVDQDAQKLDYLSR
4051 786.0477 2355.1213 2355.1563 -14.9 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
DHTVLLIEHNMSIVMSISQR + 2 Oxidation (M); Deamidated (NQ)
2328 318.1681 634.3216 634.3286 -11.0 1 5 0.88 +1Score > 22 indicates identity
Score > 17 indicates homology
DKTSGK
3254 446.8885 1337.6437 1337.6728 -21.8 1 5 0.48 +1Score > 22 indicates identity
Score > 14 indicates homology
SRWYETQLVR + Deamidated (NQ)
2462 354.1776 706.3406 706.3650 -34.4 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NLGFEK
3658 918.4555 1834.8964 1834.9676 -38.8 1 5 0.47 +1Score > 23 indicates identity
Score > 14 indicates homology
QILSNLISNAIKFTDR + 3 Deamidated (NQ)
2725 482.7189 963.4232 963.4630 -41.3 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MPAVKSCR + Oxidation (M)
3300 347.9299 1387.6905 1387.7095 -13.7 0 5 0.94 +1Score > 23 indicates identity
Score > 17 indicates homology
ELITDLNWASAR
3447 513.9229 1538.7469 1538.7801 -21.6 1 5 0.84 +1Score > 22 indicates identity
Score > 16 indicates homology
ALGRNDELAHSSIR + Deamidated (NQ)
4632 736.0005 3674.9661 3674.7919 47.4 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
TFSPNLDQMTPEQLRALAAQALQLQSQVEAMSR + 3 Deamidated (NQ)
4494 1141.2413 3420.7021 3420.6620 11.7 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
RQFDEGEEAFLVTMSAQLAGVIAHAEATGSIR + Oxidation (M); Deamidated (NQ)
3821 723.0438 2166.1096 2166.0579 23.8 1 5 0.42 +1Score > 22 indicates identity
Score > 13 indicates homology
KVAAADYLTQQQDELTLEK + 3 Deamidated (NQ)
2297 312.1847 622.3548 622.3438 17.7 0 5 1 +1Score > 17 indicates identity LYTAR
3022 1143.5800 1142.5727 1142.5655 6.33 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
TRAQSFMFR
3414 500.2807 1497.8203 1497.8092 7.39 1 5 0.8 +1Score > 22 indicates identity
Score > 16 indicates homology
KAWWLTEAGPALR
3552 571.6426 1711.9060 1711.8417 37.5 0 5 0.69 +1Score > 22 indicates identity
Score > 16 indicates homology
ELVAYFGTQEVTAER
3911 453.6155 2263.0411 2263.1458 -46.2 1 5 0.69 +1Score > 22 indicates identity
Score > 15 indicates homology
RHHEAIGQGDALNDVVLHPGK + Deamidated (NQ)
4044 784.7138 2351.1196 2351.1249 -2.27 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
VPAEAMANVRNDMYLTSEAIR + Deamidated (NQ)
4266 646.5656 2582.2333 2582.2104 8.85 1 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
QAMDMAVNKEAILQAVYQDAGQR + 2 Oxidation (M); Deamidated (NQ)
4465 679.5521 3392.7241 3392.6195 30.8 0 4 0.65 +1Score > 21 indicates identity
Score > 15 indicates homology
NACTVDAVITVVDSPAVAAGTFAAYPDQVDAQR + Deamidated (NQ)
3179 635.3127 1268.6108 1268.5819 22.8 1 4 0.62 +1Score > 21 indicates identity
Score > 15 indicates homology
EMRTGQLFDR + Oxidation (M); Deamidated (NQ)
4583 884.7158 3534.8341 3534.8100 6.82 1 4 0.55 +1Score > 21 indicates identity
Score > 14 indicates homology
DGRLRPGMTAQVSLRPGENADDSLLLPAEAVIR + Oxidation (M); 2 Deamidated (NQ)
3388 738.9002 1475.7858 1475.7845 0.93 1 4 0.81 +1Score > 22 indicates identity
Score > 16 indicates homology
LRPVQSGGGSRFSK + Deamidated (NQ)
4604 596.9838 3575.8591 3575.7969 17.4 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LGLEMTAQQIYSLLHKEYLQANAPYALVSHR + Oxidation (M); 3 Deamidated (NQ)
2810 505.2956 1008.5766 1008.5716 5.00 0 4 1.3 +1Score > 18 indicates identity LTHLADALR
4351 901.4554 2701.3444 2701.3096 12.9 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
RASWQEAASSSLTELFAPQIHQSR + 2 Deamidated (NQ)
3893 749.0820 2244.2242 2244.1970 12.1 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
LRVVLMPLSTQSEMVEQLR + Oxidation (M)
2539 398.7562 795.4978 795.4967 1.50 0 4 0.37 +1Score > 13 indicates identity LQPLLGR
3944 1142.1198 2282.2250 2282.2304 -2.36 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LVGPTGQVVLADINDSMLKVGR + Deamidated (NQ)
4569 704.9748 3519.8376 3519.7304 30.5 1 4 0.45 +1Score > 20 indicates identity
Score > 13 indicates homology
NACTVDAVITVVDSPAVAAGTFAAYPDQVDAQRK
3615 445.4695 1777.8489 1777.9151 -37.3 1 4 0.42 +1Score > 23 indicates identity
Score > 13 indicates homology
AFAKQFDLPADLTWR
2642 442.7765 883.5384 883.5239 16.4 0 4 1.1 +1Score > 17 indicates identity VGQALAGLR
3167 633.3233 1264.6320 1264.6663 -27.1 0 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
LVNTVQLTYGR + 2 Deamidated (NQ)
3739 1001.5269 2001.0392 2001.0452 -2.98 1 4 0.57 +1Score > 23 indicates identity
Score > 14 indicates homology
LLGKDVLEQLGQTMEAQK + Deamidated (NQ)
3501 549.2776 1644.8110 1644.8756 -39.3 1 4 0.69 +1Score > 23 indicates identity
Score > 15 indicates homology
SLDNKLASLSPEMLK
3474 528.6230 1582.8472 1582.7740 46.2 0 4 0.58 +1Score > 21 indicates identity
Score > 14 indicates homology
ANNFGGQGLTTFTVR + Deamidated (NQ)
2948 544.2646 1086.5146 1086.5458 -28.7 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
KDPFNPVNR + Deamidated (NQ)
3633 602.9877 1805.9413 1805.9676 -14.6 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
FEPGSFNVLLGRTLAGK + Deamidated (NQ)
3321 469.9289 1406.7649 1406.7935 -20.4 1 4 0.67 +1Score > 20 indicates identity
Score > 15 indicates homology
LVHFRLFGPHGK
3899 450.6247 2248.0871 2248.1964 -48.6 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
LGLRYDIRPDLQVYGNLSR + Deamidated (NQ)
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