| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 803.4284 | 802.4211 | 802.3821 | 48.6 | 0 | 24 | 0.062 | 1Score > 24 indicates identity |
GLDVDQR + Deamidated (NQ) | |
| 559.3206 | 558.3133 | 558.3125 | 1.39 | 0 | 23 | 0.11 | 1Score > 26 indicates identity |
INGVR + Deamidated (NQ) | |
| 531.3045 | 530.2972 | 530.2813 | 30.1 | 0 | 23 | 0.15 | 1Score > 27 indicates identity |
QGIGR + Deamidated (NQ) | |
| 301.6674 | 601.3202 | 601.3336 | -22.2 | 0 | 23 | 0.06 | 1Score > 23 indicates identity |
LAWGR | |
| 575.3156 | 574.3083 | 574.3074 | 1.52 | 0 | 22 | 0.095 | 1Score > 25 indicates identity |
QLSAR + Deamidated (NQ) | |
| 858.5053 | 857.4980 | 857.4607 | 43.6 | 0 | 22 | 0.069 | 1Score > 23 indicates identity |
VALVDGQR + Deamidated (NQ) | |
| 991.4849 | 990.4776 | 990.4982 | -20.7 | 0 | 21 | 0.07 | 1Score > 22 indicates identity |
LQSTIGSQR + 2 Deamidated (NQ) | |
| 457.7821 | 913.5496 | 913.5596 | -10.9 | 0 | 21 | 0.058 | 1Score > 21 indicates identity |
LLSAILER | |
| 393.5437 | 1177.6093 | 1177.6277 | -15.7 | 1 | 20 | 0.087 | 1Score > 22 indicates identity |
LNRMFLIDR + Deamidated (NQ) | |
| 504.2440 | 1006.4734 | 1006.4389 | 34.3 | 0 | 20 | 0.084 | 1Score > 22 indicates identity |
LGQGQMAER + Oxidation (M); 2 Deamidated (NQ) | |
| 429.7567 | 857.4988 | 857.4607 | 44.5 | 0 | 20 | 0.064 | 1Score > 23 indicates identityScore > 20 indicates homology |
VALVDGQR + Deamidated (NQ) | |
| 511.2527 | 1020.4908 | 1020.5240 | -32.5 | 0 | 20 | 0.087 | 1Score > 22 indicates identity |
LQEFLQSR + Deamidated (NQ) | |
| 539.2688 | 1076.5230 | 1076.5251 | -1.88 | 0 | 20 | 0.092 | 1Score > 22 indicates identity |
EFNQGQVVR + Deamidated (NQ) | |
| 544.3220 | 543.3147 | 543.3129 | 3.39 | 0 | 19 | 0.087 | 1Score > 21 indicates identity |
AIGQR | |
| 687.4056 | 686.3983 | 686.4188 | -29.8 | 1 | 19 | 0.13 | 1Score > 24 indicates identityScore > 23 indicates homology |
GRVVTR | |
| 862.4059 | 861.3986 | 861.4127 | -16.3 | 0 | 19 | 0.11 | 1Score > 22 indicates identity |
TACVAGAGR | |
| 465.2533 | 1392.7381 | 1392.6772 | 43.7 | 0 | 19 | 0.092 | 1Score > 21 indicates identityScore > 21 indicates homology |
LANAYIQQVDEK + 2 Deamidated (NQ) | |
| 372.2425 | 742.4704 | 742.4701 | 0.46 | 0 | 19 | 0.12 | 1Score > 23 indicates identity |
LATVALR | |
| 544.3214 | 543.3141 | 543.3129 | 2.29 | 0 | 19 | 0.097 | 1Score > 21 indicates identity |
AIGQR | |
| 317.6696 | 633.3246 | 633.3082 | 26.0 | 0 | 19 | 0.1 | 1Score > 21 indicates identity |
SNSGLR + Deamidated (NQ) | |
| 575.3165 | 574.3092 | 574.3075 | 3.06 | 0 | 19 | 0.25 | 1Score > 25 indicates identity |
QITGR + Deamidated (NQ) | |
| 316.1706 | 630.3266 | 630.3085 | 28.8 | 0 | 19 | 0.14 | 1Score > 23 indicates identity |
AGQQAR + Deamidated (NQ) | |
| 419.2394 | 836.4642 | 836.4439 | 24.3 | 1 | 19 | 0.068 | 1Score > 19 indicates identity |
HKLMHR + Oxidation (M) | |
| 515.3306 | 514.3233 | 514.3227 | 1.17 | 0 | 18 | 0.21 | 1Score > 24 indicates identity |
LAGVR | |
| 646.3326 | 645.3253 | 645.3235 | 2.89 | 0 | 18 | 0.24 | 1Score > 25 indicates identity |
DWLGR | |
| 435.7686 | 869.5226 | 869.5520 | -33.8 | 1 | 18 | 0.064 | 1Score > 19 indicates identity |
LMPLLRK | |
| 344.1935 | 686.3724 | 686.3711 | 1.91 | 0 | 18 | 0.15 | 1Score > 22 indicates identity |
QIGVDR | |
| 544.3105 | 543.3032 | 543.3129 | -17.8 | 0 | 18 | 0.11 | 1Score > 21 indicates identity |
AAQVR | |
| 610.3200 | 1218.6254 | 1218.6278 | -1.92 | 0 | 18 | 0.2 | 1Score > 23 indicates identity |
LAMLDAATELR + Oxidation (M) | |
| 634.2952 | 633.2879 | 633.3082 | -32.0 | 0 | 18 | 0.1 | 1Score > 20 indicates identity |
VSESGR | |
| 478.7388 | 955.4630 | 955.4611 | 2.08 | 0 | 18 | 0.084 | 1Score > 19 indicates identity |
LPEDTPER | |
| 566.2739 | 1130.5332 | 1130.5356 | -2.11 | 0 | 18 | 0.14 | 1Score > 22 indicates identity |
EDIQSVFHR + Deamidated (NQ) | |
| 469.2500 | 1404.7282 | 1404.7613 | -23.6 | 1 | 18 | 0.16 | 1Score > 22 indicates identity |
QVAVLFKDVTER + Deamidated (NQ) | |
| 743.4442 | 742.4369 | 742.4337 | 4.30 | 0 | 18 | 0.19 | 1Score > 23 indicates identity |
GLTLSPR | |
| 765.9090 | 1529.8034 | 1529.7824 | 13.7 | 0 | 18 | 0.17 | 1Score > 23 indicates identityScore > 22 indicates homology |
GAEEVLVESELVEK | |
| 453.7346 | 905.4546 | 905.4858 | -34.4 | 0 | 17 | 0.13 | 1Score > 23 indicates identityScore > 21 indicates homology |
LLQAFGEK + Deamidated (NQ) | |
| 1053.5216 | 1052.5143 | 1052.5138 | 0.48 | 0 | 17 | 0.14 | 1Score > 21 indicates identity |
AFTTQTNLR + 2 Deamidated (NQ) | |
| 551.7913 | 1101.5680 | 1101.5553 | 11.6 | 0 | 17 | 0.24 | 1Score > 24 indicates identity |
LNLQAGQINK + 4 Deamidated (NQ) | |
| 859.4561 | 858.4488 | 858.4487 | 0.14 | 0 | 17 | 0.23 | 1Score > 23 indicates identity |
GWLLEQI + Deamidated (NQ) | |
| 470.5591 | 1408.6555 | 1408.6517 | 2.65 | 1 | 17 | 0.17 | 1Score > 22 indicates identity |
MPHTPSSHSEKR + Oxidation (M) | |
| 414.2719 | 826.5292 | 826.5388 | -11.6 | 1 | 17 | 0.081 | 1Score > 19 indicates identity |
LQLRIGK | |
| 1089.5996 | 1088.5923 | 1088.5502 | 38.7 | 0 | 17 | 0.18 | 1Score > 22 indicates identity |
APDFAEQLAK | |
| 431.2373 | 430.2300 | 430.2288 | 2.80 | 0 | 17 | 0.12 | 1Score > 20 indicates identity |
GGAAR | |
| 575.2935 | 574.2862 | 574.3075 | -37.0 | 0 | 17 | 0.35 | 1Score > 25 indicates identity |
QITGR + Deamidated (NQ) | |
| 509.7354 | 1017.4562 | 1017.4978 | -40.9 | 0 | 17 | 0.12 | 1Score > 20 indicates identity |
AGVLDSEAEK | |
| 508.7600 | 1015.5054 | 1015.5410 | -35.0 | 1 | 17 | 0.24 | 1Score > 23 indicates identity |
QIGRLSANR + 2 Deamidated (NQ) | |
| 547.3010 | 546.2937 | 546.2762 | 32.2 | 0 | 17 | 0.28 | 1Score > 24 indicates identity |
SNIGR + Deamidated (NQ) | |
| 431.2369 | 430.2296 | 430.2288 | 1.87 | 0 | 17 | 0.11 | 1Score > 20 indicates identityScore > 20 indicates homology |
GGAAR | |
| 672.4050 | 671.3977 | 671.4078 | -15.1 | 1 | 17 | 0.23 | 1Score > 23 indicates identity |
DRLIR | |
| 589.3679 | 588.3606 | 588.3595 | 1.92 | 0 | 17 | 0.11 | 1Score > 20 indicates identity |
LTSIR | |
| 317.6692 | 633.3238 | 633.3082 | 24.7 | 0 | 17 | 0.16 | 1Score > 21 indicates identity |
SNSGLR + Deamidated (NQ) | |
| 597.8161 | 1193.6176 | 1193.6148 | 2.38 | 0 | 17 | 0.16 | 1Score > 21 indicates identity |
IGLLMDMTLR + 2 Oxidation (M) | |
| 496.2471 | 990.4796 | 990.4982 | -18.7 | 0 | 17 | 0.2 | 1Score > 22 indicates identity |
LQSTIGSQR + 2 Deamidated (NQ) | |
| 1124.5577 | 1123.5504 | 1123.5622 | -10.4 | 1 | 17 | 0.17 | 1Score > 21 indicates identity |
TERFSQSLR + Deamidated (NQ) | |
| 547.3010 | 546.2937 | 546.2762 | 32.2 | 0 | 17 | 0.29 | 1Score > 24 indicates identity |
SNIGR + Deamidated (NQ) | |
| 431.2371 | 430.2298 | 430.2288 | 2.33 | 0 | 17 | 0.13 | 1Score > 20 indicates identity |
GGAAR | |
| 589.3677 | 588.3604 | 588.3595 | 1.58 | 0 | 17 | 0.12 | 1Score > 20 indicates identity |
LTSIR | |
| 842.4994 | 841.4921 | 841.4658 | 31.3 | 0 | 16 | 0.16 | 1Score > 21 indicates identity |
VTEPALGR | |
| 747.8579 | 1493.7012 | 1493.7613 | -40.2 | 0 | 16 | 0.18 | 1Score > 21 indicates identity |
ISQLNGSINIFSAK + 3 Deamidated (NQ) | |
| 560.2733 | 559.2660 | 559.2714 | -9.65 | 0 | 16 | 0.12 | 1Score > 20 indicates identityScore > 19 indicates homology |
VGNDR | |
| 821.7569 | 2462.2489 | 2462.2111 | 15.3 | 1 | 16 | 0.22 | 1Score > 22 indicates identity |
LPSIGGVNLSQMGNAQGAAEKQFK + Oxidation (M); 2 Deamidated (NQ) | |
| 464.2854 | 926.5562 | 926.5913 | -37.8 | 1 | 16 | 0.11 | 1Score > 20 indicates identityScore > 19 indicates homology |
KVLAELVR | |
| 434.2113 | 866.4080 | 866.4102 | -2.50 | 1 | 16 | 0.15 | 1Score > 20 indicates identity |
MIRMER + 2 Oxidation (M) | |
| 556.3298 | 1110.6450 | 1110.6549 | -8.90 | 0 | 16 | 0.086 | 1Score > 18 indicates identity |
AILNALPPFR | |
| 413.2647 | 824.5148 | 824.4868 | 34.0 | 0 | 16 | 0.053 | 1Score > 16 indicates identity |
QLPLAQR | |
| 630.8183 | 1259.6220 | 1259.5993 | 18.0 | 0 | 16 | 0.25 | 1Score > 22 indicates identity |
ASIQNEADNGLK + Deamidated (NQ) | |
| 488.2834 | 487.2761 | 487.2754 | 1.41 | 0 | 16 | 0.43 | 1Score > 25 indicates identity |
NAGVK | |
| 464.7897 | 927.5648 | 927.5250 | 43.0 | 1 | 16 | 0.13 | 1Score > 20 indicates identity |
QRLLNQR + Deamidated (NQ) | |
| 672.3688 | 671.3615 | 671.3602 | 1.95 | 0 | 16 | 0.15 | 1Score > 20 indicates identity |
NLTPAR + Deamidated (NQ) | |
| 516.3147 | 515.3074 | 515.3180 | -20.5 | 1 | 16 | 0.48 | 1Score > 25 indicates identity |
GKGVR | |
| 633.3239 | 1264.6332 | 1264.6420 | -6.92 | 1 | 16 | 0.23 | 1Score > 23 indicates identityScore > 22 indicates homology |
IAHYMKAIMR + 2 Oxidation (M) | |
| 1045.5906 | 1044.5833 | 1044.5451 | 36.6 | 0 | 16 | 0.26 | 1Score > 22 indicates identity |
EELGELLSR | |
| 338.1827 | 674.3508 | 674.3460 | 7.24 | 1 | 16 | 0.33 | 1Score > 23 indicates identity |
GSRAER | |
| 527.2838 | 1052.5530 | 1052.5978 | -42.5 | 0 | 15 | 0.13 | 1Score > 21 indicates identityScore > 19 indicates homology |
LVANPQLAAR + Deamidated (NQ) | |
| 516.2245 | 1030.4344 | 1030.4791 | -43.4 | 1 | 15 | 0.062 | 1Score > 16 indicates identity |
QRNEEQAR + Deamidated (NQ) | |
| 650.3268 | 649.3195 | 649.3217 | -3.40 | 0 | 15 | 0.19 | 1Score > 21 indicates identity |
ALGMSR + Oxidation (M) | |
| 372.8530 | 1115.5372 | 1115.5645 | -24.5 | 0 | 15 | 0.24 | 1Score > 22 indicates identity |
SMPPEILSAR + Oxidation (M) | |
| 877.3998 | 876.3925 | 876.4123 | -22.6 | 0 | 15 | 0.15 | 1Score > 19 indicates identity |
MLNAEQR + Oxidation (M) | |
| 377.8687 | 1130.5843 | 1130.5567 | 24.4 | 0 | 15 | 0.24 | 1Score > 23 indicates identityScore > 21 indicates homology |
LQSEQQQLR + 2 Deamidated (NQ) | |
| 516.2561 | 1030.4976 | 1030.4791 | 18.0 | 1 | 15 | 0.29 | 1Score > 22 indicates identity |
QRNEEQAR + Deamidated (NQ) | |
| 505.2301 | 1008.4456 | 1008.4699 | -24.0 | 0 | 15 | 0.13 | 1Score > 19 indicates identity |
GAMPFDLSR + Oxidation (M) | |
| 452.2138 | 902.4130 | 902.4570 | -48.7 | 1 | 15 | 0.18 | 1Score > 20 indicates identity |
NDTLRER | |
| 607.2637 | 606.2564 | 606.2795 | -38.1 | 0 | 15 | 0.12 | 1Score > 18 indicates identity |
SMVDR | |
| 569.9738 | 1706.8996 | 1706.8886 | 6.42 | 1 | 15 | 0.27 | 1Score > 22 indicates identityScore > 22 indicates homology |
LHMQVPGAGLRQVQR + Oxidation (M); 2 Deamidated (NQ) | |
| 432.2477 | 862.4808 | 862.4661 | 17.1 | 0 | 15 | 0.14 | 1Score > 20 indicates identityScore > 19 indicates homology |
AGLLNGYR | |
| 612.3296 | 1222.6446 | 1222.6492 | -3.72 | 0 | 15 | 0.2 | 1Score > 22 indicates identityScore > 20 indicates homology |
QIINLHTMPR + Deamidated (NQ) | |
| 812.4004 | 811.3931 | 811.3824 | 13.2 | 0 | 15 | 0.1 | 1Score > 17 indicates identity |
DHAQDVK | |
| 516.7612 | 1031.5078 | 1031.5400 | -31.1 | 0 | 15 | 0.081 | 1Score > 23 indicates identityScore > 16 indicates homology |
AVQQYGIPR + Deamidated (NQ) | |
| 480.5455 | 1438.6147 | 1438.6432 | -19.8 | 0 | 15 | 0.13 | 1Score > 18 indicates identity |
QMSPMSAEATVVR + 2 Oxidation (M); Deamidated (NQ) | |
| 557.3666 | 556.3593 | 556.3697 | -18.6 | 0 | 15 | 0.22 | 1Score > 20 indicates identity |
LAVVR | |
| 755.4672 | 754.4599 | 754.4337 | 34.7 | 0 | 15 | 0.09 | 1Score > 17 indicates identity |
AQAPLAGK | |
| 445.7291 | 889.4436 | 889.4141 | 33.2 | 0 | 15 | 0.34 | 1Score > 22 indicates identity |
QANQALSR + 3 Deamidated (NQ) | |
| 458.7239 | 915.4332 | 915.4297 | 3.82 | 0 | 15 | 0.16 | 1Score > 21 indicates identityScore > 19 indicates homology |
LEQPSQGR + 2 Deamidated (NQ) | |
| 487.2411 | 972.4676 | 972.5128 | -46.4 | 0 | 14 | 0.28 | 1Score > 22 indicates identityScore > 21 indicates homology |
LIDAIDQGK + Deamidated (NQ) | |
| 445.7352 | 889.4558 | 889.4505 | 6.05 | 1 | 14 | 0.36 | 1Score > 23 indicates identity |
ETAANAKGK + Deamidated (NQ) | |
| 622.2623 | 621.2550 | 621.2541 | 1.55 | 0 | 14 | 0.063 | 1Score > 15 indicates identity |
GEGGMR + Oxidation (M) | |
| 395.1914 | 788.3682 | 788.4028 | -43.8 | 1 | 14 | 0.35 | 1Score > 22 indicates identity |
NQIQRK + 3 Deamidated (NQ) | |
| 669.3466 | 1336.6786 | 1336.6809 | -1.67 | 1 | 14 | 0.32 | 1Score > 22 indicates identityScore > 22 indicates homology |
LMEARNQFLAK + Oxidation (M); Deamidated (NQ) | |
| 490.2467 | 978.4788 | 978.4440 | 35.6 | 0 | 14 | 0.22 | 1Score > 22 indicates identityScore > 20 indicates homology |
ELEQMASR + Oxidation (M) | |
| 336.7123 | 671.4100 | 671.4078 | 3.29 | 1 | 14 | 0.26 | 1Score > 23 indicates identityScore > 21 indicates homology |
RIEVR |
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