MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4617 730.3948 3646.9376
4618 730.5946 3647.9366
4619 912.9919 3647.9385
4620 608.9971 3647.9389
4621 608.9973 3647.9401
4622 522.1420 3647.9431
4623 608.9982 3647.9455
4624 915.7698 3659.0501
4625 733.0186 3660.0566
4626 611.4949 3662.9257
4627 735.9907 3674.9171
4628 919.7394 3674.9285
4629 613.4959 3674.9317
4631 613.5005 3674.9593
4634 613.5041 3674.9809
4635 736.0039 3674.9831
4636 736.1929 3675.9281
4637 919.9904 3675.9325
4638 736.1939 3675.9331
4639 613.6631 3675.9349
4640 613.6637 3675.9385
4641 613.6639 3675.9397
4643 919.9941 3675.9473
4644 613.6652 3675.9475
4645 736.1979 3675.9531
4646 613.6668 3675.9571
4647 613.6669 3675.9577
4648 736.1991 3675.9591
4649 920.0199 3676.0505
4650 736.2223 3676.0751
4651 920.2391 3676.9273
4653 923.9904 3691.9325
4654 616.3295 3691.9333
4655 739.3953 3691.9401
4656 925.2361 3696.9153
4661 743.7845 3713.8861
4662 929.4798 3713.8901
4663 744.5995 3717.9611
4664 746.9803 3729.8651
4666 623.1665 3732.9553
4671 748.4125 3737.0261
4673 940.9971 3759.9593
4675 943.5006 3769.9733
4676 755.7897 3773.9121
4679 759.6089 3793.0081
4680 949.5076 3794.0013
4681 761.6120 3803.0236
4682 634.8468 3803.0371
4683 635.0120 3804.0283
4684 544.4400 3804.0291
4685 761.8135 3804.0311
4686 635.0129 3804.0337
4687 544.4407 3804.0340
4688 544.4411 3804.0368
4691 952.0175 3804.0409
4692 761.8163 3804.0451
4693 761.8169 3804.0481
4695 544.4430 3804.0501
4699 954.2721 3813.0593
4700 637.6801 3820.0369
4702 958.5258 3830.0741
4703 958.5308 3830.0941
4704 958.5309 3830.0945
4705 962.5276 3846.0813
4706 771.4148 3852.0376
4707 964.0219 3852.0585
4708 644.3517 3860.0665
4709 968.0118 3868.0181
4710 645.6777 3868.0225
4711 774.6126 3868.0266
4712 777.6044 3882.9856
4713 777.9866 3884.8966
4714 972.7839 3887.1065
4715 784.8024 3918.9756
4716 792.8187 3959.0571
4717 996.4861 3981.9153
4720 1068.7882 4271.1237
4722 725.9095 4349.4133
4723 1102.3330 4405.3029
4729 1106.3307 4421.2937
4730 1108.5820 4430.2989
4731 1108.5867 4430.3177
4732 1108.5879 4430.3225
4733 1108.5889 4430.3265
4734 887.2676 4431.3016
4735 1110.0814 4436.2965
4736 888.4630 4437.2786
4737 1112.5798 4446.2901
4738 1112.5875 4446.3209
4740 1113.8269 4451.2785
4741 891.6627 4453.2771
4742 1118.0699 4468.2505
4743 894.6611 4468.2691
4744 1143.3645 4569.4289
4745 915.0963 4570.4451
4746 1147.8549 4587.3905
4748 975.5168 4872.5476
4749 1025.5154 5122.5406
4750 1025.7058 5123.4926
4751 1056.7544 5278.7356
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