MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4426 830.1860 3316.7149
4427 665.2106 3321.0166
4428 666.5362 3327.6446
4429 555.6150 3327.6463
4430 835.4483 3337.7641
4431 835.9453 3339.7521
4433 670.3513 3346.7201
4434 837.6914 3346.7365
4435 674.0165 3365.0461
4444 846.4391 3381.7273
4445 1130.6123 3388.8151
4460 1131.5905 3391.7497
4463 1131.5933 3391.7581
4467 1132.9000 3395.6782
4470 568.3098 3403.8151
4475 681.9703 3404.8151
4479 1136.5856 3406.7350
4480 852.9413 3407.7361
4481 682.5547 3407.7371
4483 1138.5690 3412.6852
4484 854.1793 3412.6881
4486 1138.9138 3413.7196
4487 854.4388 3413.7261
4488 683.9478 3414.7026
4489 855.6904 3418.7325
4492 855.9395 3419.7289
4495 1141.2446 3420.7120
4496 856.1863 3420.7161
4498 1141.5822 3421.7248
4500 686.1502 3425.7146
4503 1143.2411 3426.7015
4504 1143.5692 3427.6858
4505 857.9310 3427.6949
4506 857.9332 3427.7037
4508 858.1792 3428.6877
4509 686.7449 3428.6881
4510 686.7481 3428.7041
4511 1144.2372 3429.6898
4513 1145.5481 3433.6225
4518 1146.5835 3436.7287
4519 1146.9092 3437.7058
4520 1148.5685 3442.6837
4521 1148.5688 3442.6846
4522 861.6796 3442.6893
4523 1148.5707 3442.6903
4524 861.9196 3443.6493
4525 1148.8950 3443.6632
4526 861.9266 3443.6773
4528 689.9385 3444.6561
4530 575.2841 3445.6609
4531 690.3680 3446.8036
4533 862.9486 3447.7653
4534 1150.5935 3448.7587
4536 1150.8805 3449.6197
4537 1150.9130 3449.7172
4538 1151.2424 3450.7054
4539 863.6896 3450.7293
4540 864.1747 3452.6697
4541 865.6698 3458.6501
4542 1153.8988 3458.6746
4543 865.9203 3459.6521
4544 865.9257 3459.6737
4545 1154.2349 3459.6829
4546 1154.5760 3460.7062
4548 1156.5532 3466.6378
4550 867.9159 3467.6345
4551 869.6702 3474.6517
4552 700.6321 3498.1241
4553 584.4776 3500.8219
4557 876.9974 3503.9605
4572 587.6473 3519.8401
4574 1174.2885 3519.8437
4576 880.9703 3519.8521
4577 587.6495 3519.8533
4584 707.9756 3534.8416
4585 590.3127 3535.8325
4587 1181.6115 3541.8127
4588 591.3688 3542.1691
4590 887.9680 3547.8429
4591 710.5765 3547.8461
4592 1185.2701 3552.7885
4593 711.7667 3553.7971
4594 712.3632 3556.7796
4595 593.8043 3556.7821
4597 890.4554 3557.7925
4598 1187.6198 3559.8376
4599 891.4624 3561.8205
4602 1188.6151 3562.8235
4603 1188.6305 3562.8697
4605 716.1799 3575.8631
4606 894.9756 3575.8733
4607 716.3804 3576.8656
4608 1193.6122 3577.8148
4610 895.4612 3577.8157
4611 895.4638 3577.8261
4612 895.4639 3577.8265
4613 901.2498 3600.9701
4614 724.8128 3619.0276
4615 905.7655 3619.0329
4616 912.5126 3646.0213
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