MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4295 886.0974 2655.2704
4296 886.0982 2655.2728
4297 886.4263 2656.2571
4298 886.4286 2656.2640
4299 665.0734 2656.2645
4300 665.0745 2656.2689
4301 886.7620 2657.2642
4302 665.3268 2657.2781
4304 887.0989 2658.2749
4306 665.5767 2658.2777
4307 887.1001 2658.2785
4308 665.5770 2658.2789
4309 665.5781 2658.2833
4311 665.8232 2659.2637
4312 887.4360 2659.2862
4315 889.0947 2664.2623
4316 668.5753 2670.2721
4317 891.0992 2670.2758
4318 668.5767 2670.2777
4319 668.5787 2670.2857
4320 668.8262 2671.2757
4321 891.7607 2672.2603
4322 669.0789 2672.2865
4324 892.4318 2674.2736
4325 669.8246 2675.2693
4328 537.0540 2680.2336
4329 894.4260 2680.2562
4330 671.3181 2681.2433
4331 894.7865 2681.3377
4332 894.8080 2681.4022
4333 896.4319 2686.2739
4334 672.5768 2686.2781
4335 672.6166 2686.4373
4336 538.2951 2686.4391
4337 896.8032 2687.3878
4338 672.8546 2687.3893
4339 673.0992 2688.3677
4340 674.0670 2692.2389
4341 899.0767 2694.2083
4342 674.5594 2694.2085
4343 539.8503 2694.2151
4344 675.0629 2696.2225
4345 899.7488 2696.2246
4346 540.2525 2696.2261
4347 675.8332 2699.3037
4348 675.8384 2699.3245
4350 901.4195 2701.2367
4352 901.7805 2702.3197
4354 679.0554 2712.1925
4355 679.8310 2715.2949
4357 908.1659 2721.4759
4358 914.5089 2740.5049
4359 687.6569 2746.5985
4362 923.1715 2766.4927
4364 923.8253 2768.4541
4366 927.8431 2780.5075
4367 696.1345 2780.5089
4368 698.8293 2791.2881
4369 561.6937 2803.4321
4370 701.8654 2803.4325
4371 937.1713 2808.4921
4372 937.4870 2809.4392
4373 706.3918 2821.5381
4377 952.5303 2854.5691
4378 958.4921 2872.4545
4379 719.3705 2873.4529
4380 719.3857 2873.5137
4381 719.3861 2873.5153
4382 962.5041 2884.4905
4383 729.6506 2914.5733
4384 976.8246 2927.4520
4385 736.6296 2942.4893
4387 738.1271 2948.4793
4390 743.9032 2971.5837
4391 595.3256 2971.5916
4392 744.4061 2973.5953
4393 992.8682 2975.5828
4394 995.2189 2982.6349
4396 747.8911 2987.5353
4397 999.3489 2995.0249
4398 1000.4302 2998.2688
4400 1000.5135 2998.5187
4401 1003.5480 3007.6222
4402 603.0988 3010.4576
4403 1005.6754 3014.0044
4404 755.4324 3017.7005
4405 609.5314 3042.6206
4406 769.6854 3074.7125
4407 772.9021 3087.5793
4408 805.6805 3218.6929
4409 1077.8586 3230.5540
4412 1085.1832 3252.5278
4413 1087.5873 3259.7401
4414 1090.1732 3267.4978
4415 818.4196 3269.6493
4416 655.1537 3270.7321
4417 1092.1807 3273.5203
4420 656.4073 3277.0001
4423 1095.8342 3284.4808
4425 1101.2555 3300.7447
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