MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4152 815.1051 2442.2935
4153 408.2236 2443.2979
4154 815.4590 2443.3552
4156 612.8138 2447.2261
4165 818.0746 2451.2020
4167 614.0639 2452.2265
4168 818.4192 2452.2358
4172 820.4269 2458.2589
4173 615.5730 2458.2629
4174 492.6604 2458.2656
4175 820.4537 2458.3393
4177 820.7817 2459.3233
4178 821.0854 2460.2344
4180 821.4642 2461.3708
4181 616.3508 2461.3741
4182 616.5681 2462.2433
4184 821.7560 2462.2462
4185 616.5689 2462.2465
4186 616.5694 2462.2485
4188 616.5704 2462.2525
4189 616.5709 2462.2545
4191 823.7468 2468.2186
4192 824.0731 2469.1975
4194 824.0895 2469.2467
4195 824.4144 2470.2214
4198 619.8055 2475.1929
4199 826.0721 2475.1945
4200 826.1014 2475.2824
4202 620.0736 2476.2653
4203 827.0885 2478.2437
4205 827.0902 2478.2488
4208 829.4119 2485.2139
4210 622.3118 2485.2181
4211 622.3131 2485.2233
4212 498.2493 2486.2101
4215 830.8833 2489.6281
4216 831.0914 2490.2524
4218 623.7971 2491.1593
4219 499.2397 2491.1621
4220 831.4155 2491.2247
4221 831.7617 2492.2633
4222 624.0731 2492.2633
4223 624.8943 2495.5481
4224 500.1169 2495.5481
4225 833.0920 2496.2542
4226 500.8459 2499.1931
4227 626.0549 2500.1905
4229 834.7347 2501.1823
4230 501.2447 2501.1871
4232 835.7715 2504.2927
4235 837.0652 2508.1738
4236 837.0745 2508.2017
4237 837.0931 2508.2575
4238 628.0726 2508.2613
4240 629.7972 2515.1597
4241 504.2412 2516.1696
4242 839.7308 2516.1706
4243 840.0865 2517.2377
4244 630.5681 2518.2433
4245 840.7639 2519.2699
4246 630.8265 2519.2769
4247 840.7734 2519.2984
4248 631.7988 2523.1661
4249 842.0630 2523.1672
4250 842.4770 2524.4092
4252 632.8287 2527.2857
4253 844.1296 2529.3670
4254 633.5604 2530.2125
4255 633.5897 2530.3297
4257 849.1252 2544.3538
4260 853.4207 2557.2403
4262 856.1378 2565.3916
4263 857.8130 2570.4172
4264 858.1271 2571.3595
4265 644.8506 2575.3733
4267 863.4460 2587.3162
4268 648.0858 2588.3141
4269 863.7800 2588.3182
4270 865.1487 2592.4243
4271 651.6018 2602.3781
4272 868.7998 2603.3776
4273 654.5693 2614.2481
4274 525.7335 2623.6311
4278 877.4853 2629.4341
4279 880.4240 2638.2502
4280 660.5717 2638.2577
4281 880.4624 2638.3654
4282 880.4757 2638.4053
4283 661.3253 2641.2721
4284 881.7499 2642.2279
4285 881.7677 2642.2813
4286 881.7681 2642.2825
4287 661.5782 2642.2837
4288 881.7688 2642.2846
4289 661.5801 2642.2913
4290 661.8251 2643.2713
4291 882.0997 2643.2773
4292 661.8286 2643.2853
4293 883.3259 2646.9559
4294 885.4786 2653.4140
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