MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3775 694.0670 2079.1792
3781 700.6378 2098.8916
3783 1051.0868 2100.1590
3784 1051.5503 2101.0860
3785 701.3998 2101.1776
3786 702.3541 2104.0405
3788 527.2684 2105.0445
3789 1054.5293 2107.0440
3791 706.3680 2116.0822
3796 708.0320 2121.0742
3798 708.3581 2122.0525
3799 709.0470 2124.1192
3800 710.3918 2128.1536
3801 1065.5859 2129.1572
3804 717.3557 2149.0453
3805 717.3564 2149.0474
3810 1079.5593 2157.1040
3811 1079.5967 2157.1788
3812 720.0670 2157.1792
3813 540.3026 2157.1813
3814 720.0681 2157.1825
3818 722.7008 2165.0806
3819 722.7129 2165.1169
3823 1086.0289 2170.0432
3824 726.4180 2176.2322
3825 545.0659 2176.2345
3826 727.3926 2179.1560
3828 1093.0559 2184.0972
3831 731.0667 2190.1783
3834 732.7164 2195.1274
3836 1100.5613 2199.1080
3838 1101.0807 2200.1468
3840 736.0283 2205.0631
3841 1103.5399 2205.0652
3842 736.3799 2206.1179
3843 1105.5833 2209.1520
3855 1106.0833 2210.1520
3858 1107.5679 2213.1212
3859 554.5523 2214.1801
3860 1109.0743 2216.1340
3861 1110.0950 2218.1754
3863 1111.0338 2220.0530
3864 741.0270 2220.0592
3865 741.3569 2221.0489
3866 1111.8451 2221.6756
3868 1112.5708 2223.1270
3872 1114.5936 2227.1726
3873 1115.5845 2229.1544
3874 744.4185 2230.2337
3875 1116.1261 2230.2376
3877 1116.5649 2231.1152
3881 745.0455 2232.1147
3882 745.0462 2232.1168
3883 1119.5757 2237.1368
3884 1120.0673 2238.1200
3885 747.0523 2238.1351
3886 747.0538 2238.1396
3887 1120.0807 2238.1468
3888 747.0577 2238.1513
3890 1122.6217 2243.2288
3896 450.6237 2248.0821
3898 750.3690 2248.0852
3906 751.7474 2252.2204
3907 752.0359 2253.0859
3915 567.3065 2265.1969
3917 756.7233 2267.1481
3919 756.7257 2267.1553
3924 1136.1195 2270.2244
3925 568.5634 2270.2245
3926 757.7493 2270.2261
3927 758.0193 2271.0361
3928 569.0193 2272.0481
3929 1137.6447 2273.2748
3930 758.7681 2273.2825
3931 758.7770 2273.3092
3935 759.7566 2276.2480
3936 571.3141 2281.2273
3938 457.4515 2282.2211
3939 761.7477 2282.2213
3940 571.5630 2282.2229
3941 761.7486 2282.2240
3945 761.7490 2282.2252
3949 457.4530 2282.2286
3952 457.6493 2283.2101
3953 762.0779 2283.2119
3958 762.7420 2285.2042
3959 1143.6102 2285.2058
3960 1143.6234 2285.2322
3961 572.5128 2286.0221
3962 763.0167 2286.0283
3963 572.5555 2286.1929
3964 458.2460 2286.1936
3965 1144.6375 2287.2604
3966 763.4276 2287.2610
3968 764.6988 2291.0746
3969 765.0751 2292.2035
3970 1147.6041 2293.1936
3972 765.4260 2293.2562
3973 574.3221 2293.2593
3974 765.7269 2294.1589
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