MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3523 844.8845 1687.7544
3524 563.9702 1688.8888
3525 563.9786 1688.9140
3526 847.3806 1692.7466
3527 847.8819 1693.7492
3529 565.9913 1694.9521
3534 568.6815 1703.0227
3536 427.0140 1704.0269
3547 854.8699 1707.7252
3550 571.2872 1710.8398
3554 860.9434 1719.8722
3555 574.6605 1720.9597
3556 861.9391 1721.8636
3557 862.3871 1722.7596
3558 575.2613 1722.7621
3560 575.3079 1722.9019
3569 433.2788 1729.0861
3571 433.7365 1730.9169
3573 579.6503 1735.9291
3576 436.2858 1741.1141
3577 349.4303 1742.1151
3578 436.7823 1743.1001
3579 582.0413 1743.1021
3583 582.2966 1743.8680
3584 437.1777 1744.6817
3585 582.9020 1745.6842
3586 873.9327 1745.8508
3591 875.9624 1749.9102
3596 878.4457 1754.8768
3597 440.2803 1757.0921
3598 880.9556 1759.8966
3600 587.6502 1759.9288
3601 588.2260 1761.6562
3609 590.9780 1769.9122
3610 886.2120 1770.4094
3611 886.4312 1770.8478
3613 593.6210 1777.8412
3614 889.9305 1777.8464
3618 594.9922 1781.9548
3619 595.6935 1784.0587
3620 893.4355 1784.8564
3624 600.6548 1798.9426
3625 601.2836 1800.8290
3626 901.4717 1800.9288
3642 909.4822 1816.9498
3646 607.3206 1818.9400
3656 610.8270 1829.4592
3657 917.4970 1832.9794
3669 616.6531 1846.9375
3670 462.7428 1846.9421
3671 925.9551 1849.8956
3672 926.4926 1850.9706
3674 617.9981 1850.9725
3678 464.0139 1852.0265
3680 619.3124 1854.9154
3686 621.3129 1860.9169
3688 621.6425 1861.9057
3693 934.4384 1866.8622
3698 625.9920 1874.9542
3699 626.0010 1874.9812
3702 470.5417 1878.1377
3703 627.3873 1879.1401
3704 627.6837 1880.0293
3708 628.2825 1881.8257
3709 471.7144 1882.8285
3710 472.2947 1885.1497
3711 629.3916 1885.1530
3715 950.5226 1899.0306
3716 475.8045 1899.1889
3717 380.8451 1899.1891
3718 635.0138 1902.0196
3719 958.8833 1915.7520
3720 962.4758 1922.9370
3721 644.3906 1930.1500
3722 483.5448 1930.1501
3724 648.7271 1943.1595
3725 486.7973 1943.1601
3728 973.5403 1945.0660
3729 975.4987 1948.9828
3730 653.7075 1958.1007
3731 490.7826 1959.1013
3734 656.0390 1965.0952
3735 984.5273 1967.0400
3736 986.5142 1971.0138
3738 662.0225 1983.0457
3741 501.7617 2003.0177
3742 669.0140 2004.0202
3743 669.0280 2004.0622
3744 1003.5126 2005.0106
3745 1004.8833 2007.7520
3748 674.0105 2019.0097
3749 674.0505 2019.1297
3750 1011.0728 2020.1310
3753 1016.5287 2031.0428
3754 1018.5140 2035.0134
3755 680.0163 2037.0271
3756 681.6533 2041.9381
3761 685.6949 2054.0629
3762 1029.0924 2056.1702
3773 1038.5647 2075.1148
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