MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3240 440.2785 1317.8137
3241 659.9146 1317.8146
3242 440.2791 1317.8155
3244 441.5714 1321.6924
3246 665.2595 1328.5044
3255 672.7776 1343.5406
3256 449.8867 1346.6383
3265 678.8864 1355.7582
3268 454.2678 1359.7816
3276 684.8424 1367.6702
3278 685.3548 1368.6950
3280 458.2444 1371.7114
3282 460.2532 1377.7378
3283 689.8766 1377.7386
3285 690.3438 1378.6730
3286 691.8893 1381.7640
3287 692.8284 1383.6422
3301 463.5905 1387.7497
3303 348.2187 1388.8457
3307 697.8590 1393.7034
3311 467.5713 1399.6921
3312 700.8627 1399.7108
3322 469.9297 1406.7673
3326 705.8424 1409.6702
3328 471.9687 1412.8843
3329 707.4510 1412.8874
3330 471.9705 1412.8897
3331 354.4756 1413.8733
3332 354.9758 1415.8741
3341 476.2500 1425.7282
3342 477.6302 1429.8688
3344 358.7296 1430.8893
3353 716.9500 1431.8854
3355 479.2661 1434.7765
3359 481.2976 1440.8710
3362 724.8707 1447.7268
3366 727.4094 1452.8042
3367 727.4480 1452.8814
3368 485.3025 1452.8857
3369 364.7437 1454.9457
3370 365.2367 1456.9177
3371 730.3783 1458.7420
3372 487.3050 1458.8932
3373 487.3055 1458.8947
3374 730.8783 1459.7420
3375 487.6396 1459.8970
3376 487.6396 1459.8970
3378 368.2185 1468.8449
3379 735.4300 1468.8454
3380 490.6225 1468.8457
3383 368.7401 1470.9313
3384 368.9886 1471.9253
3385 491.9780 1472.9122
3386 491.9836 1472.9290
3387 492.9113 1475.7121
3392 739.8384 1477.6622
3395 493.6203 1477.8391
3401 744.9671 1487.9196
3402 496.9806 1487.9200
3403 372.9874 1487.9205
3404 373.2376 1488.9213
3406 497.6501 1489.9285
3429 505.2717 1512.7933
3430 505.2725 1512.7957
3431 757.4052 1512.7958
3432 379.9535 1515.7849
3433 758.8998 1515.7850
3435 763.4061 1524.7976
3436 509.9274 1526.7604
3444 769.4387 1536.8628
3445 769.4399 1536.8652
3455 387.9993 1547.9681
3458 390.7561 1558.9953
3460 522.2665 1563.7777
3461 783.3983 1564.7820
3465 785.4208 1568.8270
3473 528.2969 1581.8689
3475 792.4427 1582.8708
3477 529.2949 1584.8629
3478 397.7575 1587.0009
3479 795.4304 1588.8462
3481 533.5925 1597.7557
3482 800.8460 1599.6774
3483 801.4084 1600.8022
3484 534.6267 1600.8583
3486 534.9421 1601.8045
3487 401.5061 1601.9953
3488 804.4004 1606.7862
3489 537.3235 1608.9487
3490 403.7834 1611.1045
3491 538.6276 1612.8610
3492 808.4427 1614.8708
3494 410.2237 1636.8657
3495 546.6301 1636.8685
3496 821.4348 1640.8550
3497 547.9596 1640.8570
3498 547.9603 1640.8591
3499 412.0088 1644.0061
3509 834.5084 1667.0022
3521 843.4125 1684.8104
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