MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2920 538.2982 1074.5818
2922 539.6887 1077.3628
2924 360.2296 1077.6670
2927 361.1752 1080.5038
2930 541.6951 1081.3756
2932 361.8432 1082.5078
2942 543.2549 1084.4952
2945 544.2623 1086.5100
2963 547.3585 1092.7024
2965 1097.1986 1096.1913
2968 549.6774 1097.3402
2969 366.7881 1097.3425
2970 549.8263 1097.6380
2975 1102.5936 1101.5863
2976 552.8454 1103.6762
2981 557.2242 1112.4338
2982 557.6638 1113.3130
2984 372.2211 1113.6415
2986 1114.6611 1113.6538
2994 560.8400 1119.6654
2995 560.8424 1119.6702
3000 563.8481 1125.6816
3002 1130.6252 1129.6179
3003 565.8167 1129.6188
3004 377.5470 1129.6192
3011 377.9134 1130.7184
3012 566.3668 1130.7190
3016 568.8250 1135.6354
3017 1136.6456 1135.6383
3023 573.8018 1145.5890
3024 382.8707 1145.5903
3031 383.9172 1148.7298
3033 576.8088 1151.6030
3034 385.1266 1152.3580
3039 583.8186 1165.6226
3040 583.8192 1165.6238
3041 389.8824 1166.6254
3042 390.1957 1167.5653
3043 584.7919 1167.5692
3061 594.8408 1187.6670
3062 397.5924 1189.7554
3063 595.8854 1189.7562
3066 596.7567 1191.4988
3067 398.5841 1192.7305
3075 404.1921 1209.5545
3076 605.7849 1209.5552
3077 303.3964 1209.5565
3078 404.1930 1209.5572
3088 608.3116 1214.6086
3094 612.3477 1222.6808
3096 409.5422 1225.6048
3097 409.8513 1226.5321
3106 616.5701 1231.1256
3119 310.3948 1237.5501
3121 310.7844 1239.1085
3122 310.7987 1239.1657
3125 621.2719 1240.5292
3126 415.5271 1243.5595
3134 416.2687 1245.7843
3135 416.5681 1246.6825
3136 624.3492 1246.6838
3141 418.1813 1251.5221
3144 418.8915 1253.6527
3147 627.8643 1253.7140
3148 627.8669 1253.7192
3150 419.5272 1255.5598
3152 628.7886 1255.5626
3153 419.5563 1255.6471
3154 629.2885 1256.5624
3160 630.8494 1259.6842
3162 421.2716 1260.7930
3163 631.4039 1260.7932
3164 316.2056 1260.7933
3171 633.7634 1265.5122
3175 423.5280 1267.5622
3184 636.7714 1271.5282
3188 425.8694 1274.5864
3189 638.8297 1275.6448
3190 639.8216 1277.6286
3191 639.8228 1277.6310
3195 641.3164 1280.6182
3196 428.1681 1281.4825
3197 641.7488 1281.4830
3199 428.1951 1281.5635
3201 322.1522 1284.5797
3202 429.2032 1284.5878
3205 429.5412 1285.6018
3207 644.7533 1287.4920
3209 646.2840 1290.5534
3213 432.2465 1293.7177
3215 649.2862 1296.5578
3216 433.5189 1297.5349
3220 433.8572 1298.5498
3221 650.3573 1298.7000
3224 651.3289 1300.6432
3225 435.2764 1302.8074
3226 652.7382 1303.4618
3231 654.7957 1307.5768
3236 439.5829 1315.7269
3238 439.5843 1315.7311
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