MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2592 839.4501 838.4428
2593 420.2610 838.5074
2595 840.4707 839.4634
2596 841.1785 840.1712
2597 421.2063 840.3980
2598 841.4058 840.3985
2605 843.5303 842.5230
2607 422.2706 842.5266
2608 843.5341 842.5268
2613 426.7348 851.4550
2615 428.2224 854.4302
2623 430.2527 858.4908
2631 435.2302 868.4458
2640 441.7753 881.5360
2643 885.9666 884.9593
2653 449.7189 897.4232
2654 450.1745 898.3344
2656 900.4654 899.4581
2660 908.4976 907.4903
2661 454.8006 907.5866
2663 457.0067 911.9988
2669 922.5728 921.5655
2670 461.7904 921.5662
2671 309.1438 924.4096
2678 932.7531 931.7458
2681 312.4968 934.4686
2683 937.6089 936.6016
2684 469.7207 937.4268
2685 938.4736 937.4663
2686 469.7410 937.4674
2689 469.7812 937.5478
2691 314.1707 939.4903
2692 470.7528 939.4910
2693 470.7535 939.4924
2697 472.2585 942.5024
2705 476.0499 950.0852
2706 951.6682 950.6609
2708 477.2096 952.4046
2709 318.4755 952.4047
2712 477.7489 953.4832
2722 480.7642 959.5138
2726 483.7671 965.5196
2727 322.8557 965.5453
2729 484.7263 967.4380
2732 968.4666 967.4593
2733 485.0803 968.1460
2741 973.3275 972.3202
2749 325.5436 973.6090
2756 490.8165 979.6184
2757 327.5479 979.6219
2763 491.7943 981.5740
2772 330.8747 989.6023
2782 497.7047 993.3948
2791 500.7056 999.3966
2793 1001.3224 1000.3151
2794 335.1481 1002.4225
2800 503.2116 1004.4086
2801 503.2980 1004.5814
2811 337.4611 1009.3615
2813 1010.4673 1009.4600
2816 338.1476 1011.4210
2818 507.2225 1012.4304
2823 1018.3488 1017.3415
2825 340.4741 1018.4005
2829 511.3244 1020.6342
2832 513.6743 1025.3340
2833 513.7912 1025.5678
2834 513.8081 1025.6016
2836 514.2374 1026.4602
2842 514.7035 1027.3924
2843 1029.3188 1028.3115
2844 515.2080 1028.4014
2848 515.7277 1029.4408
2851 516.2861 1030.5576
2852 516.3168 1030.6190
2854 518.1921 1034.3696
2855 345.7975 1034.3707
2856 1035.4590 1034.4517
2862 519.2749 1036.5352
2863 1037.5431 1036.5358
2866 346.8626 1037.5660
2868 1040.4244 1039.4171
2874 523.2199 1044.4252
2875 349.1612 1044.4618
2882 523.8037 1045.5928
2885 350.5252 1048.5538
2887 525.7618 1049.5090
2888 1052.4623 1051.4550
2894 530.2643 1058.5140
2901 1060.4022 1059.3949
2902 530.7048 1059.3950
2903 531.2043 1060.3940
2904 531.2214 1060.4282
2905 531.2217 1060.4288
2906 532.2592 1062.5038
2909 355.8508 1064.5306
2914 357.1547 1068.4423
2915 535.8208 1069.6270
2916 536.2804 1070.5462
2919 537.8369 1073.6592
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