MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2277 309.0949 616.1752
2279 309.1677 616.3208
2280 309.1680 616.3214
2281 309.1689 616.3232
2287 310.5334 619.0522
2288 621.1398 620.1325
2292 621.9932 620.9859
2295 311.6696 621.3246
2298 312.6589 623.3032
2301 313.1288 624.2430
2302 313.1292 624.2438
2305 625.4039 624.3966
2307 626.9709 625.9636
2308 314.6643 627.3140
2309 629.1771 628.1698
2310 315.1904 628.3662
2329 635.3860 634.3787
2331 640.8618 639.8545
2332 321.1729 640.3312
2338 321.1907 640.3668
2339 321.1908 640.3670
2343 321.6533 641.2920
2344 321.6643 641.3140
2350 642.4307 641.4234
2353 322.0583 642.1020
2354 643.1223 642.1150
2355 322.1742 642.3338
2356 643.3548 642.3475
2358 645.0965 644.0892
2364 325.1624 648.3102
2365 649.3670 648.3597
2370 326.1571 650.2996
2371 652.2617 651.2544
2372 652.3420 651.3347
2373 326.6994 651.3842
2374 652.4132 651.4059
2375 328.6454 655.2762
2376 329.1235 656.2324
2377 329.1241 656.2336
2379 657.3687 656.3614
2381 330.0473 658.0800
2382 659.0883 658.0810
2384 659.3483 658.3410
2390 331.1555 660.2964
2393 662.3268 661.3195
2394 663.2686 662.2613
2396 332.6746 663.3346
2400 664.4125 663.4052
2402 333.5096 665.0046
2404 336.6318 671.2490
2405 336.6436 671.2726
2411 674.0510 673.0437
2412 338.1493 674.2840
2413 675.2920 674.2847
2417 679.2393 678.2320
2418 679.4160 678.4087
2421 341.1209 680.2272
2424 342.1569 682.2992
2436 693.2349 692.2276
2439 348.6785 695.3424
2442 697.2962 696.2889
2443 349.1518 696.2890
2444 350.1770 698.3394
2448 351.1414 700.2682
2455 352.1606 702.3066
2461 705.4306 704.4233
2463 355.1384 708.2622
2465 712.2503 711.2430
2467 356.7101 711.4056
2468 713.2669 712.2596
2474 359.6341 717.2536
2475 360.1425 718.2704
2478 720.3578 719.3505
2479 361.6953 721.3760
2480 722.4565 721.4492
2483 727.3420 726.3347
2484 364.1753 726.3360
2487 729.2770 728.2697
2493 368.1285 734.2424
2494 371.6805 741.3464
2500 372.5317 743.0488
2510 757.0576 756.0503
2512 379.7034 757.3922
2518 385.6995 769.3844
2523 389.6858 777.3570
2524 391.2691 780.5236
2531 785.4072 784.3999
2533 786.9985 785.9912
2537 397.1489 792.2832
2544 801.5690 800.5617
2553 809.4889 808.4816
2554 809.5253 808.5180
2557 406.7040 811.3934
2558 406.7067 811.3988
2559 407.6615 813.3084
2564 815.3365 814.3292
2571 409.0374 816.0602
2581 827.4113 826.4040
2582 414.2093 826.4040
2586 833.1403 832.1330
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